Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/25414
標題: 烏骨雞黑色素抗氧化及保護皮膚纖維母細胞抗光老化之有效性評估
Evaluation of antioxidant activity and anti-photo aging effect of silky fowl melanin on human skin fibroblast
作者: 蕭爾欣
Hsiao, Erh-Hsin
關鍵字: silky fowl
烏骨雞
melanin
anti-oxidation
UVB
human skin fibroblast
黑色素
抗氧化
中波紫外光
人類皮膚纖維母細胞
出版社: 畜產學系所
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摘要: 本研究之目的在於探討由烏骨雞雞皮萃取出後配置成之黑色素溶液(silky fowl melanin,SS-melanin)其於體外試驗中之抗氧化特性及對生物體分子氧化之抗氧化性測定,並與化學合成黑色素(synthesis melanin, SY-melanin)相比較。此外,針對黑色素溶液對於人類皮膚纖維母細胞(WS1)細胞活性之影響及於中波紫外光(UVB)誘導下,烏骨雞黑色素溶液及合成黑色素溶液對於纖維母細胞之光保護(photoprotection)效果。 研究結果顯示,於體外試驗之抗氧化特性分析方面,黑色素溶液具有廣譜的吸光值、顯著性抗脂質過氧化能力(p<0.05)、有效之螯合亞鐵離子、清除1,1-diphenyl-2-picryl-hydrazyl(DPPH)自由基(p <0.01)、清除超氧陰離子(p<0.01)、還原力(p<0.01)、清除過氧化氫(p<0.01)之能力及可有效抑制費頓效應(Fenton reaction)所誘導之去氧核糖之氧化傷害(p<0.01)之抗氧化特性。而當黑色素溶液濃度愈高時,其抗氧化效果亦愈佳。於人類皮膚纖維母細胞(WS1)細胞之抗老化及抗光老化之試驗方面。添加黑色素溶液(20μg/mL)於培養液(DMEM)中可促進皮膚纖維母細胞增生;於紫外光UVB(312nm , 37.6 J/cm2)誘導下,黑色素溶液可有效降低紫外線UVB對皮膚纖維母細胞(WS1)之傷害、延緩細胞老化、抑制細胞中活性氧群(過氧化氫)及脂質過氧化物(丙二醛 )的產生。於細胞中抗氧化系統方面,於紫外光UVB(312nm , 37.6 J/cm2)誘導下,烏骨雞黑色素可明顯提高超氧歧化酶及過氧化氫酶的活性。此外,黑色素溶液具有促進細胞分泌膠原蛋白之能力,且於紫外光誘導下,黑色素溶液具有有效降低UVB造成膠原蛋白光降解之保護特性。黑色素溶液於紫外光UVB(312nm , 37.6 J/cm2)誘導下可有效降低皮膚纖維母細胞DNA的損傷,即黑色素溶液於紫外光UVB(312nm , 37.6 J/cm2)誘導下可有效減緩DNA斷裂的程度。
The purpose of this study was to investigate the characteristics of anti-oxidizing and organic molecules anti-oxidation of melanin solution extracted from silky fowl skin(SS-melanin)comparing with Synthesis melanin(SY-melanin)solution. Besides, in the light of melanin solution on influences of cell activities and photoprotection abilities of silky fowl skin melanin solution and synthesis melanin solution induced by UVB. The results appeared that experiment in vitro,aspects of the assay on anti-oxidizing properties of silky fowl melanin (SS-melanin)solution and synthesis melanin (SY-melanin)solution, melanin solution had broad spectrum absorption, significantly anti-lipid peroxidation(p<0.05), effective chelating abilities on ferrous iron, scavenging abilities on DPPH radicals(p<0.05), scavenging abilities on superoxide anion(p<0.05), reducing abilities(p<0.05), eliminating abilities on hydrogen peroxide(p<0.05)and preventing deoxyribose from damage induced by Fenton reaction(p<0.05). And complying with the concentration of silky fowl melanin (SS-melanin)solution and synthesis melanin (SY-melanin)solution became thicker, the anti-oxidation abilities showed better results. In the experiments of human skin fibroblast(WS1)on antiaging and anti-photoaging, melanin solution could promote viaility of fibroblasts. Under irradiation of UVB(312nm , 37.6 J/cm2), melanin solution could protect fibroblast from Ultraviolet rays leison(p<0.05), delay aging of fibroblast(p<0.01), inhibit production of reactive oxygen species(ROS)(p<0.05)and lipid peroxide (malondialdehyde,MDA)(p<0.01).On the aspect of anti-oxidizing system of fibroblast, SS-melanin could increase activity of superoxidase and catalase apparently(p<0.01) under UVB(312nm , 37.6 J/cm2) irradiation. Furthermore, melanin solution could promote collagen excretion of cell(p<0.05)and diminished UVB-induced photodegradation of collagen suspension. Melanin solution also showed great ability on preventing fibroblast DNA damage under UVB irradiation(312nm , 37.6 J/cm2), that is to say, melanin solution could retard degrees of DNA fragmentation.
URI: http://hdl.handle.net/11455/25414
其他識別: U0005-2408200615401100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2408200615401100
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