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Evaluation of biocompatibility of bilayer wound dressing composed of collagen/hyaluronic acid/chitosan complex matrix and non-woven fabric
|關鍵字:||Bilayer wound dressing|
|摘要:||皮膚之修復為臨床醫學治療上重要的課題之ㄧ。鑑於深層皮膚缺損於癒合過程中常伴隨疤痕形成(scar formation)及傷口收縮(wound contraction)等影響皮膚正常功能之缺陷，因此諸如自體移植、異種移植及異體移植等治療方式於過去數十年間陸續開發出，雖可顯著提升療效，但卻難以避免來源匱乏、感染及發炎等限制與風險。故現今研究趨向運用組織工程之概念，以天然生物材料為原料，模仿皮膚基質之形狀製備三度空間基質，使細胞依著模型進行修復並生成新的組織，而達到皮膚損傷之修復(repair)或再生(regeneration)。多孔性膠原蛋白基質具有高度的生物相容性及可接受的生物可降解性，因而廣泛應用於傷口敷料及真皮替代物之製備，但其效能卻受限於快速降解及低機械強度等缺陷。
The skin trauma is one of the oldest problems in surgical field. In the past decades, many studies focused on some skin substitutes such as autografts, xenografts and allografts to treat the full-thickness skin defects. However, these substitutes would not prevent the wound healing from complication of scar formation and wound contraction. Therefore, the normal functions of the skin would be reduced prognosis. For solving the mentioned problems, the biological skin substitutes or biological dressings, which designed by the principle of tissue engineering already extensively developed. Nowadays, collagen-based matrices are considered to be the most promising substitutes. However, the utilization of untreated collagen matrices were limited due to the rapid biodegradation rate and lower mechanical strength. The purpose of this study was to manufacture a bilayer wound dressing consisting of an upper layer of non-woven fabric and sublayer of collagen /chitosan/hyaluronic acid (Col-Chi-HA) complex matrix to improve the low mechanical strength and rapid biodegrading rate of the uncross-linked collagen matrices. The Col-Chi-HA complex matrices were prepared by different ratios of collagen to chitosan (10: 0, 9: 1, 8: 2, 7: 3, 6: 4 and 5: 5) freeze-drying and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) cross-linking method in this study. The physico-chemical properties and biocompatibility of various bilayer wound dressings were characterized by microstructure observation, swelling, tensile strength, thermal stability, resistance of collagenase, MTS assay and the cell morphology observed for a period of coculture. The results showed that the morphology of the bilayer wound dressings significantly exhibited two different layers, and the sublayer had more porous structure and the average of pore diameters ranged from 138μm to 210μm. In swelling, all bilayer dressings could absorb more than 30 to 40 folds of phosphate-buffered saline buffer and maintained their form-stability. The Col-Chi-HA complex matrices mixed by the ratio of 10: 0, 9: 1 and 8: 2 (collagen:chtosan) exhibited the highest tensile strength, but not reached significant difference among them. The resistance of collagenase and thermal stability of various Col-Chi-HA complex matrices were increased by the proportion of chitosan. In biocompatibility experiment, the regular morphology, viability and proliferation of the fibroblasts can be maintained when them cultured with each Col-Chi-HA complex matrix. However, the Col-Chi-HA complex matrix prepared by the ratio of 9: 1 of collagen to chitosan (Col9Chi1HA) exhibited the highest cell viability. In conclusion of these results, we assumed that the 9: 1 mixing ratio of collagen to chitosan may used as the optimal ratio to manufacture complex matrix depending on the results of the physico-chemical properties, biocompatibility, reasonable cost and healing potential. Furthermore, the bilayer wound dresssing composed of non-woven fabric and Col9Chi1HA complex matrix could be developed as a suitable wound dresssing for wound repair in the future.
|Appears in Collections:||動物科學系|
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