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A novel glucose biosensor based on a gold nano-trees modified electrode
three-dimensional gold nanoparticle
electrochemical impedance spectroscopy
disposable three-electrode chip
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|摘要:||本研究成功的在平面金電極上藉由電化學的方式沉積出具有樹枝狀奈米結構的薄膜，為了簡化製程，本研究進一步以市售之三極式拋棄電極為基材沉積奈米金仙人掌球電極，並藉由共價鍵結合(Covalent Bonding Method)的方式在奈米金仙人掌球電極上被覆一層葡萄糖氧化酵素(Glucose Oxidase，GOD)，形成葡萄糖生物感測器，再以循環伏安法(CV)檢測溶液中葡萄糖的濃度。由於三維奈米金結構具有高生物相容性以及更大的表面積增加電子傳遞之效能，因此本研究之葡萄糖感測器具有低檢測電壓(0.06V)、高靈敏度(2.28 μA/mM．cm2)、迅速反應(1.8s)及與市售系統相容性佳等特點。|
The study has successfully developed a thin film with branch-shaped nanostructure on flat gold electrode via electrochemical impedance spectroscopy (ESI). In order to facilitate our fabrication, a commercial disposable three-electrode chip is used to be a substrate to deposit gold nanoparticles. Moreover, Glucose oxidase (GOD) is coated on the selected electrode by covalent bonding method. After the fabrication, the glucose biosensor can measure the concentration of glucose by using cyclic voltammetry (CV). In addition, three-dimensional gold nanoparticles can represent high biocompatibility and larger surface area of reaction zone to increase the efficiency of conveying electrons. As a result, the glucose biosensor has several main advantages such as low requirement of voltage (0.06V), high sensibility (2.28μA/mM．cm2), quick response (1.8 sec) and its potential market.
|Appears in Collections:||機械工程學系所|
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