Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28086
標題: 一條根(闊葉大豆Glycine tomentella)健康種苗之培育
The studies on propagating healthy I-Tiao-Gung (Glycine tomentella) plantlets
作者: Yeh, Zen-Wei
葉人維
關鍵字: 一條根
I-Tiao-Gung
闊葉大豆
健康種苗
植物組織培養
Glycine tomentella
tissue culture
healthy plantlet
出版社: 土壤環境科學系所
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摘要: Medical plants have been paid more attention recently for which are more advantages, in containing more curative compositions and having less side effects, than chemical medicines. “I-Tiao-Gung” (Glycine tomentella HAYATA), has become one among the most peculiar products in Jinmen under the cooperative promotion by the Jinmen county government and local store managers. G. tomentella is a perennial legume and unable to expand its production scale because of its short growing period in a year, low germination rate and witches' broom disease. The aims of this study were on improving its germination rate and establishing a procedure to grow healthy plantlets of G. tomentella through tissue culture. Results from the germination experiments, showed that the germination rate was increased from 25% to more than 70% and 58%, respectively, after soaking in water for 24 hours and drying at 50℃ (WD treatment) and water bath at 50℃ for 2 hours (WT treatment). The seeds can preserve for 30 days after these two treatments without loss of effectiveness. Its germination rate was increased with increasing incubation temperature after heat treatments. After a series of experiments were carried out, results showed that, the tissues of top-tip and stem-node are the highest ability in forming buds, about 90%, and the other organs, like leaf, leafstalk and stem segment could not induce any buds. The modified medium( B5S medium), with the addition of 0.5 mg L-1 BA, 0.2 mg L-1 NAA, 2% sucrose and 0.9% agar into B5 medium, can induce largest number of buds from stem-nodes of G. tomentella, reaches 3.44 buds per stem-node averagely. There was no phytoplasma found in the plantlets produced in this study, neither from the tissue culture and nor from the heat treated seeds.
藥用植物之某些特殊成分、療效具優於化學合成藥物且較無副作用等優點,近幾年來已成為全球各國學者及企業所致力開發之產業。「金門一條根」之學名為闊葉大豆(Glycine tomentella HAYATA),在金門縣政府及民間業者的合作推廣下,已發展為該地區特有的產業之一。闊葉大豆為多年生之豆科植物,在栽培上有每年營養生長期短、發芽率不一及簇葉病等問題,不利於大規模種植。本研究為培育闊葉大豆之健康種苗,進行促進種子發芽和組織培養二方面的研究。將闊葉大豆種子浸潤24小時後,分別於50℃烘乾2小時(WD),或在50℃的水浴處理2小時(WT),分別可將發芽率由25%提升至70%及58%以上。而經過處理的種子,可於室溫下保存一個月,而不降低其發芽率。不同溫度之培育試驗發現闊葉大豆種子的發芽率有隨環境溫度上昇而提高的趨勢。在組織培養方面,誘發芽之能力以莖節及莖頂最高,皆約90%,而葉片、葉柄及節間無法誘發芽體。將闊葉大豆無菌苗之莖節以B5培養基為基礎,並添加0.5 mg L-1 BA、0.2 mg L-1 NAA、2%蔗糖及0.9% agar之修改後培養基(B5S medium)中培養,可誘導出最多的芽,平均每節可誘導出3.44個以上的芽。組培及經熱處理種子所育出之苗皆未檢驗出菌質體。
URI: http://hdl.handle.net/11455/28086
其他識別: U0005-2407200712033100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2407200712033100
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