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|標題:||亞磷酸根及亞磷酸根利用菌對小白菜Brassica chinensis L. Cultiva生長的影響|
Effect of phosphite and phosphite-utilizing microorganisms on the growth of Pak-choi (Brassica chinensis L. Cultiva)
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|摘要:||Phosphite (HPO3-2, Phi) is a reduced form of phosphorus, having a similar structure to phosphate (PO4-3, Pi). The former is a diprotic acid, but phosphate is a triprotic acid. Phosphite salts have been used as a fungicide for some diseases control. Some plants can used it as phosphorus fertilizer, but no phosphorus fertilizer effect for other plants, after the long-term use it even caused ecological problem. This study using hydroponic system to investigate the effect of phosphite/phosphate ratio in the nutrient solution on the growth, nitrate reductase, phosphatase, nutrient uptake of Pak-choi (Brassica chinensis L.,Cultiva). Screening Phi using bacteria from soil and inoculated into nutrient solution to evaluate their effect on the growth of Pak-choi.
Results showed that increased the phosphite/phosphate ratio in nutrient solution decreased weights of shoot and root in fresh and dry, concentrations of extractable phosphate, total phosphorus, and magnesium, activities of leaf nitrate reductase and phosphatase, and the chlorophyll index. Evidence showed phosphate contributes a partial function as phosphorus fertilizer. Although the fresh weight and dry weight of Pak-choi root of 100％ phosphate treatment was 56 %, and 67 % decreased, respectively, compared to no phosphorus treatment. The fresh weight and dry weight of Pak-choi shoot was 69 % and 9 % increased, respectively, compared to the no phosphorus treatment.
The inoculation of the top three most effective phosphate using bacteria, increased the Pak-choi shoot and root fresh weights by 37-93 % and 27-62 %, respectively, compared to no inoculation treatment|
亞磷酸為一種還原性磷，其結構與磷酸相似，不同之處磷酸為三質子酸亞磷酸為二質子酸。亞磷酸鹽目前已被做為某些病害防治劑，但是也發現對某些作物生長有抑制現象甚至造成生態不平衡。本研究利用水耕探討亞磷酸根和磷酸根濃度比對小白菜生長及其硝酸還原酶及磷酸酶活性的影響。並且從土壤篩選可利用亞磷酸根微生物，接種至水耕系統中，觀察其對亞磷酸根養液小白菜生長的影響。 本試驗結果顯示，亞磷酸根相對濃度提高時小白菜生長量無論地上部及根鮮重及乾重皆降低。對植體總磷，抽出性磷酸根、鎂的濃度及葉綠素測值也有降低趨勢，甚至達顯著水準，植體硝酸根濃度則有提高趨勢且達顯著水準。降低葉片硝酸還原酵素及磷酸酵素的活性達顯著水。但是全施亞磷酸根小白菜莖葉產量比不施磷處理高，鮮重增加69 %，乾重增加9 %；根部鮮重降低56 %，乾重降低67 %。添加所篩選微生物處理莖葉及根鮮重分別提高37～93 %及27～62 %。
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