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Studies on Production System and Propagation Techniques of Healthy Szu Chi Tsung (Allium fistulosum L.) Seedlings
|關鍵字:||Szu Chi Tsung (Allium fistulosum L.)|
Green Onion tissue culture
以ISSR-PCR技術分析'蘭陽3號'品種實生苗之60個種子的後裔苗株，顯示，在60個種子中有60% (36/60)的種子相似性係數值在1.00，72% (43/60)的種子相似性係數值在0.97以上。此結果顯示自然授粉之'蘭陽3號'種子，其種子遺傳型質是很純正。
The results of this study reveal that winter and spring are more suitable to cultivate virus-free Szu Chi Tsung. 'Hsin Chu 821' and 'Tao Yuan No.3' performed best in the winter, and 'Lan Yang No.1' performed best in the spring-time. Also, 'Lan Yang No.1' is not suitable to be cultivated in the summer. Different crop season significantly affects horticultural characteristics such as—plant height, tiller number, leaf number, and sheath length. The viral diseases mainly affect Green Onion growth in hill weight, plant height, and leaf number. However, the viral diseases were observed to be more harmful to Pei Tsung than it is to Szu Chi Tsung. Research showed that increase in the chance of virus infection by low temperature and high humidity, and the susceptibility to viral diseases was changed in according the cultivated environments. In low land area, 'Lan Yang No.3' Pei Tsung had the highest tolerance against viral diseases. On the other hand, 'Lan Yang No.1' Szu Chi Tsung had the highest resistance against viral diseases in high altitude area. In general, seedling propagated from seeds had higher tolerance to viral infection than those from tiller. Significant correlation was found between planting density of Szu Chi Tsung and leaf number, while the effects of planting density on plant height, tiller number, sheath length, and stem diameter were dependent on the cultivars (lines). The higher plant density, the higher the leaf number and tiller number, and the less the plant diameter were found in the Szu Chi Tsung. The results of this study reveal that spring in low land area is very suitable for the seed production of Szu Chi Tsung and Pei Tsung. For 'Lan Yang No.3' Pei Tsung's production during winter in low land area, the days to seed maturation were the same for both seedlings propagated from seeds and those from tiller. Winter in middle altitude area is most suitable for seed production of 'Lan Yang No.1' Szu Chi Tsung, and is least suitable for 'Hsin Chu 821' Szu Chi Tsung. Also, the flowering and pollination periods of 'Szu Chi Tsung' often coincide with the high rainfall season in middle altitude area. The high humidity leads to occurrence of diseases, such as rust and purple blotch. They can cause the lodge of flower stalk, and severely lowering the quality and quantity of seed production. Therefore, prevention of the rain damage must be taken seriously when producing seeds for Green Onion in middle altitude area. ISSR-PCR was used to analyze genetic relationships among six Green Onion cultivar (lines). The results show that the coefficients of similarity were between 0.95 and 0.87. These six cultivars (lines) can be separated into two groups according to their genetic relationships—'Hsin Chuang', 'Lan Yang No.1', 'Lan Yang No.3', 'Hsin Chu 821', and 'Tao Yuan No.3' in one group, and 'Hsin Chu' in the second group. The first group can then be further separated into three sub-groups: 1) 'Hsin Chuang' and 'Lan Yang No.1'; 2) 'Lan Yang No.3' and 'Hsin Chu 821'; 3) 'Tao Yuan No.3'. Using ISSR-PCR to analyze 60 progenies of 'Lan Yang No.3' generating from seeds, we found that among the 60 seeds, 60% had a coefficient of similarity of 1.00, and 72% of the seeds had a coefficient of similarity higher than 0.97. This indicates that the open pollination seeds of 'Lan Yang No.3' have a high genetic purity. The shoot tips of three Szu Chi Tsung cultivars (lines), namely 'Lan Yang No.1', 'Tao Yuan No.3', and 'Hsin Chu 821', were cut and cultivated in four types of solid culture media: BP, BT1, BT2, and BT3. The results indicate that BP is the most suitable medium for shoot tip propagation; not only the survival rate is high, but also the number of shoots regenerated is high. The regenerated shoots were then transferred into the BP medium with addition of 0.1ppm NAA could optimize the rate of root regeneration. If acclimation technology of in vitro plantlets could be applied to increase plantlets survival rate, the goal to commercially producing Green Onion tissue culture seedling can be reached.
|Appears in Collections:||園藝學系|
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