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Effects of activated charcoal in vitro and chemicals treatment ex vitro on plantlet quality of Phalaenopsis.
Phalaenopsis、tissue culture、activated charcoal、 chemicals treatment、Rubisco
培養基添加不同濃度的活性碳，瓶內乙烯及二氧化碳的含量皆會隨活性碳濃度而提高，瓶內亦偵測出有甲烷的存在，對照組在繼代第60天時含有1.32 ％的甲烷，活性碳的添加可增加蝴蝶蘭瓶苗葉片的生長，葉長及葉寬分別達28.7 mm及15.4 mm，並增加根數達6.2條，根部活性可達4.04 ODA480/g F. W.，但卻會抑制根的伸長。
使用活力劑處理蝴蝶蘭出瓶瓶苗，維生素B1以澆灌方式處理出瓶瓶苗比浸置方式還要能促進植株生長，其中又以1 g/l的施用效果最好，可增加葉長以及發根，分別比對照組增加17.89 ％及4 ％，全可溶性糖的含量也會上升。硫酸鋅25 mg/l對於植株生長上的促進效果顯著，並能增加葉綠素的累積，但濃度超過50 mg/l時會出現毒害現象，200 mg/l的硫酸鋅會使葉長減少10.54 ％。甘油施用對於地上部的影響會大於地下部，0.5 ％甘油可增加葉數、葉長、葉寬以及葉片鮮重，但對於根部生長方面的影響則較不顯著。以25 g/l蔗糖溶液處理在第15天時可以加快葉片的擴張速度，葉長及葉寬比對照組多45.98 ％及31.34 ％，但在之後植株生長勢會因為糖分過多而被抑制，植株甚至出現葉片萎凋及根部死亡的情形。
探討IBA與維生素B1之間協力作用對出瓶蝴蝶蘭瓶苗生長的影響，單獨施用IBA 1 g/l可以提高植株發根數以及根部鮮重，2 g/l IBA則會造成毒害，導致根短縮、乾枯或是死亡，1 g/l維生素B1 × IBA 1 g/l可以明顯提高葉片生長，刺激發根及根的伸長，根部活性更可達4.15 ODA480/g F.W.。
比較藥劑施用對出瓶蝴蝶蘭瓶苗Rubisco酵素活性的影響，對照組在出瓶後Rubisco酵素活性僅有0.38 n mol RuBp carboxylase mg protein-1 min-1，以25 mg/l硫酸鋅處理可使Rubisco酵素活性提高為0.69 n mol RuBp carboxylase mg protein-1 min-1，但50 mg/l的硫酸鋅即會造成抑制現象，200 mg/l硫酸鋅甚至會使Rubisco酵素活性降至0.22 n mol RuBp carboxylase mg protein-1 min-1。蔗糖的施用會造成植體中碳水化合物的累積，而使Rubisco酵素活性下降，以100 g/l蔗糖溶液處理的出瓶蝴蝶蘭瓶苗在出瓶第30天，Rubisco酵素活性僅只有0.11 n mol RuBp carboxylase mg protein-1 min-1。|
The study was to seek effects of activated charcoal on Phalaenopsis plantlet growth and components of the gaseous environment in vitro, and effects of chemicals application on plantlet quality and Rubisco enzyme activity ex vitro. Adding different activated charcoal concentration in medium, ethylene and dioxide carbon content in flask increased accompanied with activated charcoal concentration. Methane can also be found in the flask. There had 1.32 % methane in control at 60 days after subculture. Activated charcoal can increase leaf length to 28.7 mm and width to 15.4 mm, respectively. Root number also increased to 6.2 and root activity gave 4.04 ODA480/g F.W, but it inhibited root elongation. Using chemicals treatment to plantlet when plantlet ex vitro. Thiamine irrigated would promote plantlet growth more than thiamine dipped. In addition, the finest performance for leaf length and root number which gave 17.89 % and 4 % were found in thiamine 1 g/l, respectively. Total soluble sugar content was increase, too. 25 mg/l ZnSO4 promoted plantlet growth and chlorophyll content, but toxic phenomenon appeared when concentration excess 50 mg/l. 200 mg/l ZnSO4 decreased leaf length 10.54 %. The effect of glycerol on leaf and shoot would more than root, 0.5 % glycerol can increase leaf length、leaf width and leaf fresh weight, but the effect of root growth were not significantly. 25 g/l sucrose solution accelerated leaf expand, leaf length and leaf width increased 45.98 % and 31.34 % that compared with control after 15 days. However, plantlet growths were restrained because sucrose accumulation, sometimes, plantlet could even appear leaf withered or root died. Synergism between IBA and thiamine for plantlet growth were studied. Using 1 g/l IBA alone promoted root number and root fresh weight. 2 g/l IBA made plantlet toxic, exhibited root length decrease, wilt or died. 1 g/l IBA × 1 g/l thiamine promoted leaf length, root number and root length significantly. Root activity gave 4.15 ODA480/g F.W. Comparing effects of chemicals for Rubisco enzyme activity of plantlet ex vitro. Rubisco enzyme activity of control plantlet gave 0.38 n mol RuBp carboxylase mg protein-1 min-1. 25 mg/l ZnSO4 promoted Rubisco enzyme activity which gave 0.69 n mol RuBp carboxylase mg protein-1 min-1, but ZnSO4 50 mg/l could cause restrainer. Rubisco enzyme activity could even give 0.22 n mol RuBp carboxylase mg protein-1 min-1 when 200 mg/l ZnSO4 used. The application of sucrose solution increased carbohydrate accumulation and result in Rubisco emzyme activity decreased. Applying 100 g/l sucrose solution treatment on plantlet for 30 days after ex vitro, Rubisco enzyme activity would gave 0.11 n mol RuBp carboxylase mg protein-1 min-1 only.
|Appears in Collections:||園藝學系|
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