Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28562
DC FieldValueLanguage
dc.contributor黃敏展zh_TW
dc.contributor陳麗筠zh_TW
dc.contributor.advisor朱建鏞zh_TW
dc.contributor.author王嘉偉zh_TW
dc.contributor.authorWang, Chia-Weien_US
dc.contributor.other中興大學zh_TW
dc.date2012zh_TW
dc.date.accessioned2014-06-06T07:30:30Z-
dc.date.available2014-06-06T07:30:30Z-
dc.identifierU0005-1608201101191600zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/28562-
dc.description.abstract本試驗使用商業品種:Kalanchoe manginii‘Wendy’、K. blossfeldiana‘Isabella’,物種:落地生根K. pinnatum(KP)、K. nyikae Engler (KN),自交系:鵝鑾鼻燈籠草綠葉型(K. garambiensis‘green leaf’)之自交品系KG 93-13、鵝鑾鼻燈籠草紫葉型(K. garambiensis‘purple leaf’)之自交品系KGP 93-31。以及利用Bryophyllum節與Kalanchoe節雜交所育成的節間雜交品系 ‘Isa × KP 94-1’、‘Isa × KP 94-3’、‘Isa × Wendy 94-1’、‘Isa × Wendy 94-2’、‘KGP × KP 93-1’、‘(KG 93-13 × KP) 98-1’、‘(KG 93-13 × KP) 98-2’等。進行GA3處理、花粉活力檢定、節間雜交與回交試驗,並調查植株與開花特性。 節間雜交子代的到花日數介於117.6~128.3天,而噴施GA3可以使植株提早約5.7~10天開花。以5%蔗糖的BK 培養基中於25℃下培養兩小時之花粉發芽率以K. garambiensis ‘green leaf 93-13’最高,可達到63.3%。其次為K. pinnatum,發芽率為59.9%,節間雜交品系‘Isa × Wendy 94-1’、‘Isa × Wendy 94-2’之花粉皆無發芽。 以K. pinnatum與K. garambiensis ‘green leaf 93-13’進行節間雜交,K. pinnatum為母本時結果率為75%,結種率為11.8%,但是發芽率為0%。以K. garambiensis ‘green leaf 93-13’為母本時,結果率70%,結種率10.4%,種子發芽率為0.9%。而節間雜交後代回交的結果,只有‘KGP × KP 93-1’、‘KGP × KP 93-1M1’、‘(KG93-13 × KP) 98-1’的回交有獲得種子,但是其種子發芽率皆為0。 節間雜交後代的特性大多介於兩親本中間。而花朵數量則具有雜種優勢明顯多於兩親本,而與落地生根雜交之後代皆無遺傳到由葉緣萌發小芽之特性。zh_TW
dc.description.abstractIn this research, Kalanchoe cutivars: Kalanchoe manginii ‘Wendy', K. blossfeldiana ‘Isabella', species: K. pinnatum, K. nyikae, the inbred Lines of K. garambiensis ‘green leaf' and K. garambiensis ‘purple leaf': KG 93-13, KGP 93-31 and the inter-sectional hybrids between K. manginii ‘Wendy', K. pinnatum which belong to sect. Bryophyllum and K. garambiensis ‘green leaf', K. garambiensis ‘purple leaf', K. blossfeldiana Poellnitz ‘Isabella' which belong to sect. Kalanchoe: ‘KGP × KP 93-1', ‘Isa × KP 94-1', ‘Isa × KP 94-3', ‘Isa × Wendy 94-1', ‘Isa × Wendy 94-2', ‘(KG93-13 × KP) 98-1', ‘(KG93-13 × KP) 98-2' were subjected to GA3 treatment, pollen viability test, inter-sectional hybridization, backcross, and examine the plant and flower characteristics. Days to flower of the inter-sectional hybrids ranged from 117.6~128.3 days, and spaying of GA3 could shorten the days to flower about 5.7~10 days. Pollens that cultured on B&K medium containing 5% sucrose and kept at 25℃ for two hours, got the highest germination rate in K. garambiensis ‘green leaf 93-13', about 63.3%, followed by K. pinnatum 59.9%, and the inter-sectional hybrids‘Isa × Wendy 94-1',‘Isa × Wendy 94-2' did not germinate. The inter-sectional cross between K. pinnatum and K. garambiensis ‘green leaf 93-13', when K. pinnatum was used as maternal parent, the fruit set was 75% and fertility rate was 11.8%, but the seeds did not germinate. In the reverse crosses, when K. garambiensis ‘green leaf 93-13' was used as maternal parent, the fruit set was 70%, fertility rate was 10.4% and germination rate was 0.9%. Only the backcross of‘KGP × KP 93-1', ‘KGP × KP 93-1M1' and ‘(KG93-13 × KP) 98-1'could obtain seeds, however, the seeds did not germinate. And the embryo culture also could not get the germinative seeds. The Morphological characters of the inter-sectional hybrids are mostly intermediate between parents, and showed hybrid vigor in the number of flower, which was more than either parent.The epiphyllous bud formation on the leaf margin was one of the conspicuous characteristics of K. pinnatum, but all the hybrids of K. pinnatum did not present this characteristics.en_US
dc.description.tableofcontents前言…………………………………………………………………………........1 前人研究 一、 長壽花的起源與分類…………………………………………..............2 二、 長壽花的育種史…………………………………………………....…..3 三、 台灣燈籠草屬之重要物種………………………………………....…..4 四、 長壽花的授粉行為……………………………………………………..5 五、 長壽花的種間雜交……………………………………………………..6 六、 種間雜交障礙的克服…………………………………………………..7 材料方法 一、 植物材料……………………………………………………………....10 二、 試驗材料繁殖與栽培…………………………………………………11 三、 GA3處理對花期之影響………………………………………………12 四、 花粉發芽率測定…………………………………………………........12 五、 節間雜交與雜交後代之回交………………………………………....12 六、 雜交後代與變異植株之選拔….....…………………………………13 七、 節間雜交品系與親本性狀調查與比較………………………………13 八、 變異植株與原株之性狀調查與比較……………………………...….13 九、 流式細胞分析……………………………………………………..…..14 十、 統計分析…………………...…………………………………..…...14 結果 一、 GA3處理對花期之影響……………………………………....………15 二、 花粉發芽率…………………………………………………………....15 三、 節間雜交與雜交後代回交親本對結果率及結種率之影響……...….15 四、 節間雜交品系與親本性狀調查與比較…………………………..…..16 五、 變異植株與原株之性狀調查與比較……………………………..…..19 六、 雜交後代選拔株性狀調查………………………………………..…..19 七、 流式細胞分析…………………………………………………….…...23 討論 一、 GA3處理對花期之影響………………………………………………24 二、 節間雜交與雜交後代回交親本對結果率及結種率之影響………..25 三、 節間雜交種之特性…………………………………………………...27 表………………………………………………………………………………..29 圖………………………………………………………………………………..39 參考文獻………………………………………………………………………..54 附錄…………………………………………………………………………...61zh_TW
dc.language.isoen_USzh_TW
dc.publisher園藝學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1608201101191600en_US
dc.subjectKalanchoeen_US
dc.subject燈籠草屬zh_TW
dc.subjectinter-sectional hybridizationen_US
dc.subjectbackcrossen_US
dc.subject節間雜交zh_TW
dc.subject回交zh_TW
dc.title燈籠草屬異節物種間的雜交zh_TW
dc.titleHybridization of Kalanchoe Species Between Different Sectionsen_US
dc.typeThesis and Dissertationzh_TW
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