Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28658
標題: 利用RAPD及形態性狀評估柿種原遺傳歧異度
Assessment of Genetic Diversity among Persimmon Germplasms by RAPD Markers and Morphological Traits
作者: 湯佳裕
Tang, Jia-Yu
關鍵字: Diospyros
柿樹屬
Morphological
RAPD
形態
逢機增幅多型性DNA
出版社: 園藝學系所
引用: 1.上原敬二。1971。樹木大圖說Ⅲ。有明書房。日本東京。pp. 630~649。 2.久保利夫。1962。原色果實圖鑑。保育社。日本大阪。pp. 33~40。 3.岸本修、石畑清武。1996。熱帶果樹-樹木作物。日本東京。pp. 220。 4.淺見与七、梶浦实、森英男、永沢勝雄。1971。新選原色果物圖說。養賢堂。日本東京。pp. 245~285。 5.農山漁村文化協會。1986。果樹全書。農山漁村文化協會。日本東京。pp. 185。 6.農山漁村文化協會。2000。果樹園藝大百科。農山漁村文化協會。日本東京。pp. 5~86。 7.遠藤融郎。1987。カキ品種名鑑。日本果樹種苗協會。日本。pp. 1~143。 8.中國科學院植物研究所。1976。中國高等植物圖鑑。中國科學院植物研究所。中國北京。pp. 302~307。 9.王者悅。1992。中國藥膳大辭典。大連出版社。中國大連。pp. 92~93。 10.台北農產運銷股份有限公司。2004。果菜運銷統計年報。臺北農業運銷股份有限公司。台灣台北。pp. 336~368。 11.朱長志。1957。中國果樹分類學。台灣省立農學院出版委員出版。台灣台中。pp. 223~226。 12.行政院農業委員會。2004。農業統計年報。行政院農業委員會。台灣台北。 pp. 92-93。 13.艾倫.J ‧科莫斯。1996。樹木圖鑑。貓頭鷹出版社。台灣台北。pp. 223~227。 14.成俊卿、楊家駒、劉鵬。1992。中國木材志。中國林業出版社。中國北京。pp. 205~210。 15.邱德文、吳家榮、夏同珩。2001。本草綱目彩色藥圖-中卷。薪傳出版社。台灣台北。pp. 622。 16.呂勝由。2001。台灣稀有及瀕危植物之分級彩色圖鑑。行政院農業委員會。台灣台北。pp. 89~90。 17.林嘉興。2001。甜柿經營與管理。台中區農業改良場特刊。50:1~11。 18.吳耕民。1981。中國溫帶果樹分類學。農業出版社。中國北京。pp. 320~338。 19.吳耕民。1993。中國溫帶落葉果樹栽培學。浙江科學技術出版社。中國浙江。pp. 595~642。 20.范念慈。1994。果樹。三民書局。台灣台北。pp. 362~368。 21.張致盛。2001。柿子品種之介紹。甜柿經營與管理。台中區農業改良場特刊。台灣台中。50:11~19。 22.倪正柱。1997。特殊穗砧組合促進柿子矮化及著果研究。提昇果樹產業競爭力研討會專集Ⅲ。臺灣省臺中區農業改良場。台灣台中。pp. 285~287。 23.華海清、宋起、李益生。2003。現代養生保健中藥大辭典。薪傳出版社。台灣台北。pp. 343~348。 24.楊遠波、劉和義。2002。臺灣維管束植物簡誌第六卷。行政院農委會。台灣台北。pp. 194~195。 25.廖勇為。1988。落葉果樹分類學。五洲。台灣台北。pp. 247~255。 26.劉業經。1970。台灣重要樹木彩色圖鑑。台灣省林務局。台灣台北。pp. 242~249。 27.劉業經。1972。台灣木本植物誌。中興大學農學院。台灣台中。pp. 534~537。 28.劉業經、呂福原、歐辰雄。1994。台灣樹木誌。國立中興大學農學院。台灣台中。pp. 544~550。 29.歐錫坤、溫英杰、謝鴻業。1997。柿品種改良。提升果樹產業競爭力研討會專輯Ⅲ。台灣台中。pp. 239~242。 30.賴明洲。1987。臺灣原生景觀樹木植栽手冊。交通部觀光局員工消費合作社。台灣台北。pp. 171~172。 31.蕭培根。1989。中國本草圖錄-卷一。北京人民衛生。中國北京。pp. 143。 32.蕭培根。1989。中國本草圖錄-卷二。北京人民衛生。中國北京。pp. 124。 33.蕭培根、連文琰。1998。原色中藥原植物圖鑑(下)。南天。台灣台北。pp. 357。 34.羅正榮、王仁梓。2001。甜柿優質豐產栽培技術彩色圖說。中國農業。中國北京。pp. 1~34。 35.于洞璐。1961。落葉果樹砧木親和力測定。台灣省立農學院園藝學系。台灣台北。pp. 1~44。 36.王勝鴻。1978。接木不親和性。科學農業。26:241~246。 37.林榮貴。2001。柿的栽培-柒 生理落果的原因及其預防對策。農業世界。215:49~52。 38.林榮貴。2001。柿的栽培-參 砧木的選擇與嫁接親和性。農業世界。211:81~83。 39.倪正柱。1994。日本柿(Diospyros kaki Thunb)培育及穗砧不親和性調查。興大園藝。19:61~69。 40.黃國華。2005。保育類中藥材基因資料庫的建立及保育類中藥材基因鑑定晶片之研發。中醫藥年報。23(7):1~20。 41.溫英杰。1995。農家要覽農作篇(二)落葉果樹七-柿。豐年社。台灣台北。pp. 191~198。 42.溫英杰。2002。參加第二屆國際柿子研討會報告。農業試驗所技術服務季刊。49:3~50。 43.溫英杰、許財誠。2003。利用DAPD技術分析桃種原親緣關係。中華農學研究。52:144~152。 44.溫英杰、劉怡伶。2004。李種原評估其親緣關係之RAPD標誌研究。中華農學研究。53:97~110。 45.趙淑妙。1992。分子生物技術在研究植物親緣關係的應用。生命科學。13~33。 46.蔡巨才。1998。柿嫁接不親和性對生長之影響。中國園藝。44(1):11~28。 47.謝東佑。2002。台灣產柿樹科植物之訂正。興大園藝。27(2):1~22。 48.謝照炘。1953。苗栗縣地方有用樹木誌。國立中興大學。台灣台中。pp. 65~66。 49.Ainsworth, C. 2000. Boys and girls come out to play: the molecular biology of dioecious plants. Ann. Bot. 86:211-221. 50.Baldwin, J. T. and R. Culp. 1941. Polyploidy in Diospyros virginana L. Amer. J. Bot. 28:942-944. 51.Bank, H. V. D., M. V. D. Bank and B. V. Wyk. 2001. A review of the use of allozyne electrophoresis in plant systematics. Biochem. Syst. Ecol. 29:469-483. 52.Cardena, R., G.R. Ashburner and C. Oropeza. 2003. Identification of RAPDs associated with resistance to lethal yellowing of the coconut (Cocos uncifera L.) palm. Sci. Hortic. 98:257-263. 53.Cohen, Y., A. Gur, Z. Barkai and A. Blumenfeld. 1991. Decline of persimmon (Diospyros kaki L.)trees on Diospyros virginiana rootstocks. Sci. Hortic. 48:61-70. 54.Das, A., B. Mondal, J. Sarkar and S. Chaudhuri. 2004. RAPD profiling of some elite clones of mandarin orange (Citrus reticulate Blanco) in the North Eastern Himalayan Region of India. 2004. J. Hortic. Sci. Bio. 79:850-854. 55.Elisiario, P. J., E. M. Justo and J. M. Leitao. 1999. Identification of mandarin hybrids by isozyme and RAPD analysis. Sci. Hortic. 81:287-299. 56.Ermel, F.F., J. Kervella, A.M. Catesson and J.L. Poessel. 1999. Localized graft incompatibility in pear/quince (Pyrus communis/Cydonia oblonga) combinations: multivariate analysis of histological data from 5-month-old grafts. Tree Physiol. 19:645-654. 57.Fukuda, S., J. Nagato, T. Yamamoto, N. Hiehata and O. Terai. 2002. Cultivar identification in loquat assessed by RAPD. J. Japan. Soc. Hor. Sci. 71:826-828 58.Gebhardt, K. and W. Feucht. 1982. Polyphenol changes at the union of prunus avium / prunus cerasus grafts. J. Hort. Sci. 57:253-258. 59.Gliman, E. F. and D. G. Waston. 1993. Diospyros virgriana. Forest Service. 231:1-3. 60.Guo, D., H. Zhang and Z. Luo. 2006. Genetic relationships of Diospyros kaki Thunb. And related species revealed by IRAP and REMAP analysis. Plant Sci. 170:528-533. 61.Hartmann, H.T., D.E. Kester, F.T. Davies, and R.L. Geneve. 1997. Plant propagation: Principles and Practices. The biology of grafting. pp. 395. prentice-Hall, Upper Saddle River, N. J. 62.Hedick, P. Q. 1984. Population biology: The evolution and ecology of populations. pp. 445. Jones and Bartlett, Inc. U.S.A. 63.Heinkel, R., W. Hartmann and R. Stosser. 2000. On the origin of the plum cultivars ‘Cacaks Beauty’,‘Cacaks Best’,‘Cacaks Early’and‘Cacaks Fruitful’as investigated by the inheritance of random amplified polymorphic DNA(RAPD) fragments. Sci. Hortic. 8:149-155. 64.Inoue, E., M. Kasumi, F. Sakuma, H. Anzai, K. Amano and H. Hara. 2006. Identification of RAPD marker linked to fruit skin color in Japanese pear (Pyrus pyrifolia Nakai). Sci. Hortic. 107:254-258. 65.Jun, J. H., K. H. Chang, S. B. Jeong and H. J. Lee. 2004. An RAPD marker linked to the pollen sterility gene ps in peach (Prunus persica). J. Hortic. Sci. Bio. 79:587-590. 66.Kanzaki, S., K. Yonemori and A. Sugiura. 2001. Identification of molecular markers linked to the trait of natural astringency loss of Japanese persimmon (Diospyros kaki) Fruit. J. Amer. Soc. Hort. Sci. 126:51-55. 67.Koshita, Y., Y. Nakamura, S. Kobayashi and K. Morinaga. 2002. Introduction of the rolC gene in to the genome of the Japanese persimmon causes dwarfism. J. Japan. Soc. Hort. Sci. 71:529-531. 68.Kumar, L. S. 1999. DNA markers in plant improvement: An overview. Biotech. Advance. 17:143-182. 69.Lin. Y., X. Dexing, L. Shanjun, L. Laishui, and Y. Fanghong. 1998. The anatomical feasture observation of ‘Fuyu’ persimmon grafted with some rootstocks. Acata Agriculture Universitatis Jiangxiensis. 20:394-397. 70.Lin. Y., X. Wang and L. Lin. 2004. Analysis of genetic variation in surviving apple shoots following cryopreservation by vitrification. Plant Sci. 166:677-685. 71.Lu, S. Y. and Y. P. Yang. 1988. A revision of the Ebenaceae of Taiwan. Bull Taiwan Fot. Res. Inst. New Series. 3:131-140. 72.Luo, Z. R., K. Yonemori and A. Sugiura. 1995. Evaluation of RAPD Analysis for cultivar identification of persimmons. J. Japan. Soc. Hort. Sci. 64:535-541. 73.Luo, S., P. He, X. Zheng and P. Zhou. 2002. Inheritance of RAPD markers in an interspecific F1 hybrid of grape between Vitis quiquangularis and V. vinifera. Sci. Hortic. 93:19-28. 74.Maki, S., K. Oyama, T. Kurahashi, T. Nakahira, T. Kawabata and T. Yamada. 2001. RFLP analysis for cultivar identification of persimmon. Sci. Hortic. 91:407-412. 75.Modgil, M., K. Mahajan, S.K. Chakrabarti, D.R. Sharma and R.C. Sobti. 2005. Molecular analysis of genetic stability in micropropagated apple rootstock MM106. Sci. Hortic. 104:151-160. 76.Moing, A. and J.P. Carde. 1988. Growth, cambial activity and phloem structure in compatible and incompatible peach plum grafts. Tree Physiol. 4(4):347-359. 77.Moore, R., 1984. A model for graft compatibility-incompatibility in higher plants. Amer. J. Bot. 71:752-758. 78.Mueller, U. G., L. L. Wolfenbarger. 1999. AFLP genotyping and fingerprinting. Tree 14:389-394. 79.Nakamura, Y. and S. Kobayashi. 1994. DNA restriction fragment length variability in Diospyros kaki and related Diospyros species. Hortscience. 29(7):809-811. 80.Oliveira, C.M., M. Mota, L. Monte-Corvo, L. Goulao and D.M. Silva. 1999. Molecular typing of Pyrus based on RAPD markers. Sci. Hortic. 79:163-174. 81.Onguso, J. M., E. M. Kahangi, D. W. Ndiritu and F. Mizutani. 2004. Genetic characterization of cultivated bananas and plantains in Kenya by RAPD markers. Sci. Hortic. 99:9-20. 82.Osaka, T. 2000. Cuting propagation of Diospyros rhombifolia. Acta Hort. 517:167-174. 83.Pina, A. and P. Errea. 2005. A review of new advances in mechanism of graft compatibility-incompatibility. Sci. Hortic. 106:1-11. 84.Pooler, M.R. and R. Scorza. 1995. Aberrant transmission of RAPD markers in haploids, doubled haploid, and F1 hybrids of peach: observations and speculation on causes. Sci. Hortic. 64:233-241. 85.Ravishankara, M.N., N. Shrivastava, M.G. Jayathirtha, H. Padh, and M. Rajani. 2000. Sensitive high-performance thin-layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana Roxb. J. Chromatogr. Sci. 744:257-262. 86.Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Sci. 239:487-491. 87.Saiki, R. K., S. Scharf, F. Faloona, K. B. Mullis, G. T. Horn, H. A. Erlich and N. Arnheim. 1985. Enzymatic amplification of β-globin genomic seqences and restriction site analysis for diagnosis of sickle cell anemia. Sci. 230:1350-1354. 88.Sakanaka, S., Y. Tachibana and Y. Okada. 2005. Perparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food. Chem. 89:569-575. 89.Sharma, H. C., J. H. Crouch, K. K. Sharma, N. Seetharama and C. T. Hash. 2002. Applications of biotechnology for crop improvement: prespects and constraints. Plant Sci. 163:381-395. 90.Sugiura, A., Ryutaro T. and Takashi T., 1988. Distinguishing between Japanese persimmon cultivars (Diospyros kaki L.) by means of pollen isozymes. Sci. Hortic. 36:67-77. 91.Suzuki, T., S. Someya, F. Hu and M. Tanokura. 2005. Comparative study of catechin compositions in five Japanese persimmons (Diospyros kaki). Food. Chem. 93:149-152. 92.Tamura, M., R. Tao and A. Sugiura. 1995. Regeneration of somatic hybrids from electrofused protoplasts of Japanese persimmon (Diospyros kaki L.). Plant Sci. 108:101-107. 93.Tamura, M., R. Tao and A Sugiura. 1998. Production of somatic between Diospyros glandulosa and D. kaki by protoplast fusion. Plant cell, tissue and organ culture. 54:85-91. 94.Tao, R. and Akira S., 1987. Cultivar identification of Japanese persimmon by leaf isozymes. HortScience. 22:932-935. 95.Tetsumura, T. 2000. Cutting propagation of Diospyros rhombifolia. Acta Hort. 517: 167-174. 96.Uma, S., S. Sudha, M. S. Saraswathi, M. Manickavasagam, R. Selvarajan, P. Durai, S. Sathiamoorthy and S. A. Siva. 2004. Analysis of genetic diversity and phylogenetic relationships among indigenous and exotic Silk (AAB) group of bananas using RAPD markers. J. Hortic. Sci. Bio. 79: 523-527. 97.Upadhyay, A., K. Jayadev, R. Manimekalai and V. A. Parthasarathy. 2004. Genetic relationship and diversity in Indian coconut accessions based on RAPD markers. Sci. Hortic. 99:353-362. 98.Wang, Y., J. Chen, J. Lu and O. Lamikanra. 1999. Randomly amplified polymorphic DNA analysis of Vitis soecies and Florida bunch grapes. Sci. Hort. 82:85-94. 99.Willams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid. Res. 18:6531-6535. 100.Williams, J. G. K., R. S. Reiter, R. M. Young and P. A. Scolonik. 1993. Genetic mapping of mutations using phenotypic pools and mapped RAPD markers. Nucl. Acid. Res. 21:2697-2702. 101.Yamada, M., K. Yoshinaga, H. Yamane and T. Sumi. 1997. Dwarfing effect of the Rootstock of Diospyros rhombifolia Hemsl. On the tree growth of ‘Fuyu’ Japanese persimmon. Acta Hort. 436:295-304. 102.Yamagishi, M., S. Matsumot, A. Nakatsuka and H. Itamura. 2005. Identification of persimmon (Diospyros kaki)cultivars and phonetic relationships between Diospyros species by more effective RAPD analysis. Sci. Hort. 105:283-290. 103.Yonemori, K., D. E. Parfitt, S. Kanzaki, A. Sugiura, N. Utsunomiya and S. Subhadrabanhu. 1996. RFLP analysis of an amplified region of cpDNA for phylogeny of the genus Diospyros. J. Japan. Soc. Hort. Sci. 64:771-777. 104.Yonemori, K., S. Kanzaki, D. E. Parfitt, N. Utsunomiya, S. Subhadrabandhu and A. Sugiura. 1998. Phylogenetic relationship of Diospyros kaki (persimmon) to Diospyros spp. (Ebenaceae) of Thailand and four temperate zone Diospyros spp. From an analysis of RFLP variation in amplified cpDNA. Genome 41:173-182. 105.Zhuang, D. H., A. Kitajima, M. Ishida and Y. Sobajima. 1990. Chromosome numbers of Diospyros kaki cultivars. J. Japan. Soc. Hort. Sci. 59:289-297. 106.Zhengrong, L., K. Yonemori and A. Sugiura. 1998. Study on the genetic relationship among persimmon cultivars by RAPD. J. Fruit Sci. 15:311-316. 107.FAO. 2006. http://www.fao.org/.
摘要: 本研究共調查柿樹科柿樹屬(Diospyros)中7個種共45個柿子種原之外部形態特性,並以RAPD分子標誌進行柿品種間遺傳相似度與其鑑別能力分析。試驗結果顯示柿種間形態差異性大,而若延伸到品種間,部分品種如富有柿和上西早生富有則不易依外部形態分辨,而利用RAPD則克服外部形態辨識不足之缺點,並有效分析種間遺傳相似度關係。 形態特性上,顯示新葉顏色、花性、果重、果色、單寧含量在品種間有較大差異性,可以供作辨別種原之特殊性狀,如新葉顏色四周柿為淡褐綠色;砧木用與台灣原生柿植株均為雌雄異株,而南瓜柿具有特殊兩性花;菱葉柿具有特殊的菱形葉;蘭嶼柿果實成熟後為墨綠黑色,果肉為黑色;筆柿果形為窄橢圓形,Aoso為卵圓形;伽羅果實頂端同心圓裂痕為深裂。 RAPD分析上,所得增殖DNA片段經UPGMA建構相似度樹狀圖分析可將供試驗柿種原分成二主群,第一主群內由D. virginiana、D. japonica、D. rhombifolia、D. morrisiana、D. kotoensis、大葉砧木組成,第二主群由D. oldhamii、D. kaki、日本豆柿組成;可以發現D. virginana與台灣原生柿親緣關係上屬於同一群,而D. oldhamii親緣關系則與栽培品種較接近,顯示演化上D. oldhamii是屬於較接近栽培品種(D. kaki)。 由本研究可知,利用外部形態如新葉顏色、花性、果形等品種差異大的性狀可以將大部分的柿種原作初步分辨,而RAPD則可以有效將供試驗的柿品種作有效區隔,並且得知品種間之遺傳相似度,有助於了解種原間之相似關係。
This study discusses genetic diversity of 45 persimmons germplasm in 7 Diospyros species of Ebenaceae by morphological traits and RAPD (randomly amplified polymorphic DNA). The results show that the morphological variation of 45 persimmon was significant, some varieties ex. Fuyu and Wueinishi Wase Fuyu can't easy to make out by morphological traits. Using RAPD can solve defect and analysis the genetic diversity. In morphology characteristics, the result shows of variation in leaflet color, flower sex expression, fruit weight, fruit color, and tannins volume were large. Some special characteristics can be used of cultivars distinguish, like leaflet color of ‘Shy Jou' was pale brown green. For rootstock persimmon kinds and Taiwan Native persimmon were dioecism, and ‘Nab Gua' was hermaphrodite. In leaf shape of D. rhombifolia was diamond. D. kotoensis was greenish black in fruit color and black in pulp. In fruit shape of ‘Fude Gaki' was narrow ellipse, ‘Aoso' was the egg circular. In concentric cracking around apex of ‘Kayro' was strong. In RAPD analysis, DNA bands construct the construction similar tree by UPGMA cluster analysis could divide of 45 persimmons into 2 groups. First group constitutes by D. virginiana, D. japonica, D. rhombifolia, D. morrisiana, D. kotoensis, and ‘Da Ye'. Second group constitutes by D. oldhamii, D. kaki, rootstock of Japan. It indicates the genetic relationship of D. virginiana and Taiwan native persimmon were in the same group, and the genetic relationship of D. oldhamii was close to D. kaki. It indicates D. oldhamii was closer to D. kaki in evolution. In this study, persimmon germplasm could be done for basis identifiotion by morphology characteristics as leaflet color, flower sex expression, and fruit shape. RAPD showed effective to identify 45 persimmon cultivars in this study, it is helpful for analysis the germplasm genetic diversity.
URI: http://hdl.handle.net/11455/28658
其他識別: U0005-1707200614182700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1708200611423500
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