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Studies on the production and cultivation of virus free shallot(
Chen, Yu Chuang
|關鍵字:||shoot apex culture|
最適當之莖頂大小為帶一葉原體(約 300 m大小),其成活率及獲得無病毒
機率,以37 每日處理16小時,32 每日處理8小時,連續八週處理可獲
得100%之無病毒植汙.'佳里''品系之莖頂培養最適培養基為 MS 添加
Picloram 0.14 mg/l 與 BA 8.6 mg/l, 及 NAA 0.4 mg/l 與 TDZ 0.03
mg/l .'泰國''品系之莖頂培養最適培養基為MS添加 Picloram 0.03 mh/l
與 TDZ 1.0 mg/l.分蔥'佳里'','學甲'','福建''及'S-28''品系以莖頂培養所
Local and introduced shallot cultivars 'hia-Yi'''hwei-Chia,'' Fu-Chen'','S-28'','Philippine'' and 'Thailand'' were used to study the infuluence of shoot apex size,number of weeks in heat therapy and growth regulator for obtaining the highest propagation rate and best culture condition of producing virus- free seedings. When shoot apex with one leaf primordium (approximately 300 m)hadbetter virus-free and survival rate. If shoot apex culture with heat therapycould increase the virus- free rate. One handred percent virus-free plantlet could be obtained when shallot plant subjected to 37 for 16 hours and 32 for 8 hours daily continued for 8 weeks. The best culture medium for 'Chia-Yi'' shallot was the MS medium with Picloram 0.140 mg/l,and BA 8.6 mg/l, or with Picloram 0.39 mg/l, and TDZ 0.03 mg/l, with NAA1.1 mg/l and BA 5.3 mg/l, as well as with NAA 0.4 mg/l and TDZ 0.3 mg/l. The best culture medium for 'Thailand'' shallot shoot apex was the MS medium with Picloram 0.03 mg/l and TDZ 1.0mg/l. Yield trial in the field and storage test from virus-free plantletoriginated from shoot apex culture showed plant hight was better than ordinary plants. Number of tiller. average plant of number of bulbs per plant, and singlebulb weight of virus-free plant were higher than ordinary plant. The bulb weight decereased in ordinary bulbs was three times higher than the virus-freebulbs after 6 weeks storage was 3 times heavier of that of normal bulb.
|Appears in Collections:||園藝學系|
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