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標題: 孤挺花幼花序器內培養之體胚發生
Somatic embryogenesis from cultured developing inflorescence of Hippeastrum in vitro
作者: 洪志忠
Hong, Jhih-Jhong
關鍵字: Hippeastrum
developing inflorescence
somatic embryogenesis
出版社: 園藝學系所
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摘要: 本研究以孤挺花五個品種(Hippeastrum hybridum Hort. cvs. ‘Apple Blossom’、‘Blossom Peacock’、‘Lemon Lime’、‘Mrs. Garfield’、‘Red Lion’)為材料,藉由不同培植體、植物生長調節劑組合及培養環境等方法,探討孤挺花不定芽再生、癒傷組織、體胚形成及再生植株獲得的過程,建立一個完善的植株再生系統。 以總花序長5-15公分、培植體正置及黑暗培養4周後光照處理者最能誘導癒傷組織及不定芽形成。子房及小花梗培植體分別培養於1/2MS基礎鹽類添加NAA(0、1、2mg/l)及BA(0、1、2 、4mg/l)之組合,小花梗比子房能獲得更多胚性癒傷組織及不定芽再生,以‘Lemon Lime’品種小花梗培植體以2mg/l NAA及4mg/l BA所誘導6.89個最多。體胚形成及植株再生方面,2 mg/l 2,4-D最能使幼花序培植體形成胚性癒傷組織及體胚形成。0.0.1-0.1 mg/lTDZ能使胚性癒傷組織形成體胚率達73.33%,移至無生長調節劑的培養基中可促進體胚成熟。30-45g/l的蔗糖濃度能促進體胚的生長及成熟。
To establish an efficient plant regeneration system, this study is to investigate the effects of explants, plant growth regulators and condition on including adventitious shoots, callus, suspension culture and plant regeneration of Hippeastrum ‘Apple Blossom’,‘Blossom Peacock’,‘Lemon Lime’,‘Mrs. Garfield’,‘Red Lion’. The explants of positionⅠand positionⅡ、explant orientation normal and explant in the dark for 4weeks and transfered on the same medium in the light can induce callus and adventitious shoot formation. The explants of ovary and pedicel of Hippeastrum were used for callus and adventitious shoot induction on 1/2MS basal medium containing NAA(0,1,2mg/l) and BA(0,1,2,4mg/l). The results showed that the pedicel was better explants than ovary explants cultured on medium had higher rates of embryogenic calli and adventitious shoots. The best result was found in that ‘Lemon Lime’ pedicel explant formation 6.89 adventitious shoots in medium containing 2mg/l NAA and 4mg/l BA. To induce embryogenic calli and somatic embryogenesis, the immature inflorescence were cultured on the basal media containing 2 mg/l 2,4-D. TDZ 0.0.1-0.1 mg/l could induce the somatic embryo from embryogenic calli. when somatic embryo were culture on the medium without plant growth regulators, somatic embryogenesis, mature and regenerated plantlets could be obtained somatic embryogenesis, mature and regenerated plantlets could be obtained on media containing 30-45g/l sucrose.
其他識別: U0005-0808200713370800
Appears in Collections:園藝學系



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