Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28929
標題: 豔紅鹿子百合鱗片培養及量化增殖與生產高品質幼苗
Scale culture and mass propagation of Lilium speciosum Thunb. var. gloriosoides Baker for produce superior quality seedling
作者: 錢昌聖
Chien, Chang-Sheng
關鍵字: 豔紅鹿子百合
Lilium speciosum Thunb. var. gloriosoides Baker
鱗片培養
增殖
大量繁殖
scale culture
proliferation
mass propagation
出版社: 園藝學系所
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摘要: 鹿子百合(Lilium speciosum Thunb.)天然分佈於台灣、中國大陸及日本。台灣原產的鹿子百合屬於變種,又稱豔紅鹿子百合(L. speciosum Thunb. var. gloriosoides Baker),目前豔紅鹿子百合尚未建立一套優良的繁殖體系,故本試驗以建立完整的繁殖體系為目標,並試驗不同生長期、休眠期與低溫貯藏期的鱗莖,對鱗片培養再生能力與幼苗品質的影響;此外還試驗細胞分裂素、體外不同直徑的小鱗莖、培養溫度、十字對切與培養代數對鱗片器內增殖培養的影響。 不同生長期、休眠期與低溫貯藏期的鱗莖處理,其鱗片內可溶性醣類與澱粉含量並未直接影響鱗片的再生能力。以1月份與低溫貯藏1個月的鱗莖處理(生長期屬於抽葉期),其鱗片培養可再生形成4個小鱗莖。再生小鱗莖於試管內根部的發育情形會明顯影響幼苗品質。以7月份與低溫貯藏2個月的鱗莖處理,於器內可形成6條根、直徑9 mm以上與鮮重1.5 g的幼苗。幼苗種植於培養土後,以16.5~21.9℃的栽培溫度較能促進幼苗生長、累積鮮重以及形成地上莖。BA為影響鱗片器內增殖培養較為顯著的細胞分裂素。體外小鱗莖的直徑不會影響鱗片器內增殖時的再生能力。培養溫度會明顯影響鱗片器內增殖培養再生小鱗莖的發育,以21℃的培養溫度較能促進再生小鱗莖生長,且能維持鱗片增殖3代後的再生能力。十字對切而成的培植體於器內增殖培養可再生形成高品質的小苗,且經繼代培養後可形成直徑9.3 mm與鮮重1.5 g的幼苗。 以不同生長期或低溫貯藏後,呈現不同生長狀態的豔紅鹿子百合鱗莖作為指標時,較能調控鱗片培養的再生能力。因此選用合適生長期的鱗莖,如1月份與低溫貯藏1個月的鱗莖處理,能促進鱗片再生形成較多的小鱗莖;選用7月份與低溫貯藏2個月的鱗莖處理,則能生產高品質的幼苗。此外,16.5~21.9℃的栽培溫度較適合幼苗生長。培養基內添加0.1 mg l-1 BA時,雖然會明顯抑制鱗片器內增殖培養再生小苗葉片與根部發育,但對於隨後的繼代培養或器內增殖均可達到省工的效用。使用體外直徑較小的小鱗莖進行鱗片器內增殖培養,可縮短植物材料的養成時間,且不會影響再生能力。十字對切的處理可作為提升培植體於器內增殖培養再生能力與幼苗品質的優良方法。
Lilium speciosum Thunb. is native to Taiwan, China, and Japan and there is still no optimal propagation system of L. speciosum Thunb. var. gloriosoides Baker. Therefore, the purpose of this study is to develop a complete propagation system of this species. We have examined the influences of growth stage, dormancy and low temperature storage bulb on the regeneration ability and plantlet quality of scale culture in vitro. Another I examined the factors, including cytokinin, in vitro bulblet diameter, culture temperature, cross bisection and culture generation of scale culture for proliferation in vitro. The contents of soluble sugars and starch in scale during different growth stage, dormancy and low temperature storage bulb had no direct relationship with the regeneration ability. The bulb derived from January and low temperature storage 1 month on scale culture could regenerate 4 plantlets average one explant. The growth stage of July and low temperature storage 2 months bulb on scale culture regenerated seedlings with more vigors roots, 9 mm in diameter and 1.6 g fresh weight. After cultivated in soil, 16.5~21.9℃ was optimal for seedling growth, accumulate fresh weight and emergence. In the cytokinins of scale culture for proliferation in vitro, BA is the more influential one. The bulblet diameter of in vitro had no effect of the regeneration ability in scale culture for proliferation. Culture temperature significantly influenced the growth of regenerated bulblet in the scale culture for proliferation. When the culture at 21℃, it significantly enhanced the regenerated bulblet growth and keep the regeneration ability after subculture third generation. Cross-bisected explants would regenerat superior quality plantlets with diameter in 9.3 mm and fresh weight of 1.5 g after subcultured in vitro. Select bulb growth stage and the during of low temperature storage as explant to test the regeneration ability of scale culture. The growth stage of January and low temperature storage 1 month bulb could regenerated more bulblets derived from scale culture, another growth stage such as July and low temperature storage 2 month bulb could produce plantlet with superior quality. After cultivated in soil, 16.5~21.9℃ was optimal the seedling growth. When the basal medium was supplemented with 0.1 mg l-1 BA, the leaves and roots growth was reduced with plantlets regenerated from scale culture. The morphological plantlet could decrease the labor for subsequent subculture and proliferation. In vitro smaller bulblet used as explants could shorten the culture period and would not affect the regeneration ability from scale culture for proliferation. The optimal method of cross bisection treatment would increase the regeneration ability and seedling quality for explants during proliferation culture in vitro.
URI: http://hdl.handle.net/11455/28929
其他識別: U0005-0708200810214400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1201200917081800
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