Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29087
標題: 報歲蘭‘達摩’種子發芽及根莖器官分化之研究
Studies on the Seed Germination and Rhizome Organogenesis of Cymbidium sinense 'Da-mo' in vitro.
作者: 黃瓊慧
Huang, Chiung-Hui
關鍵字: Cymbidium sinense
報歲蘭
seed germination
rhizome differentiation
種子發芽
根莖分化
出版社: 園藝學系所
引用: 朱根發、葉秀斌、陳明莉、王碧青。2003。培養基不同成分對墨蘭根狀莖分化成苗的影響。中南林學院學報 23:42-45。 利幸貞。1992。一、素心蘭與四季蘭之無菌播種 二、溫度對四季蘭開花之研究。國立台灣大學園藝學研究所碩士論文。 呂依倫。1988。素心蘭與鳳蘭之無菌播種與器官分化。國立台灣大學園藝學研究所碩士論文。 呂依倫、李哖。1990。鳳蘭之無菌發芽。中國園藝 36:198-209。 呂依倫、李志仁、李哖。1992。培養基成份對素心蘭種子無菌發芽之影響。中國園藝 38:161-169。 李志仁。1991。報歲蘭與素心蘭開花與種子無菌發芽之研究。國立台灣大學園藝學研究所碩士論文。 李哖。1990。蘭之胚培養。中國園藝 36:223-244。 李勇毅、李哖。2001。果莢成熟度、培養基組成分與液體培養對芭菲爾鞋蘭屬之Paphiopedilum primulinum種子發芽之影響。中國園藝 47:147-156。 李哖、陳美惠。1997。拖鞋蘭之果莢成熟度和種子前處理對無菌發芽之影響。p.630-644。園藝種苗科技研究成果發表會專集。 吳新棋、李哖。1991。紅花鶴頂蘭之無菌發芽。中國園藝 37:183-198。 易美秀、王才義。2000。文心蘭無菌播種之研究。興大園藝 25:93-102。 林家榮。2007。國蘭(C. sinense ‘Shi-Ba-Xyue-Shi’× C. formosanum)根莖增殖與芽體再生體系之建立。國立宜蘭大學園藝學研究所碩士論文。 林漢榮。2003。達摩蘭藝。p.6-9。台灣達摩蘭藝協會編印。台中市。 林讚標。1988。台灣蘭科植物2。p.126-127。南天書局有限公司出版。台北市。 周鎮。1986。台灣蘭圖鑑 地生蘭篇。p.8-43。興台彩色印刷股份有限公司。台中市。 凃美智、李哖。1987。蝴蝶蘭授粉適期與果莢成熟度對種子發芽之影響。中國園藝 33:190-200。 凃美智、李哖。1988。氮素、蔗糖濃度及光強度對蝴蝶蘭種子發芽及幼苗生長之影響。中國園藝 34:293-302。 徐權君。2008。黃道報歲蘭之授粉、種子發育與發芽以及報歲蘭之品種特性比較。國立中興大學園藝學研究所碩士論文。 莊錦華、李哖。1986。活性碳、蔗糖與無機鹽類濃度對臺灣一葉蘭種子發芽與小苗生長之影響。中國園藝 32:61-69。 張正、陳盈君、嚴新富。2005。金稜邊蘭種子發芽形態研究。植物種苗 7:47-55。 郭珮琪。2006。蕙蘭產業現況及發展。p.4-11。蕙蘭栽培管理手冊。行政院農業委員會動植物防疫檢疫局。台北市。 陳美惠。1996。果莢成熟度、種子前處理及培養基成分對拖鞋蘭無菌播種與幼苗生長之影響。國立台灣大學園藝學研究所碩士論文。 陳裕星、張莉欣。2006。蕙蘭屬植物型態、種原分類及生育特性。p.12-42。蕙蘭栽培管理手冊。行政院農業委員會動植物防疫檢疫局。台北市。 許文章、蔡智賢、廖成康。1999。報歲蘭利用根莖組織繁殖種苗之研究。嘉義技術學院學報 67:1-12。 黃敏展。1976。蘭花的無菌播種。臺灣蘭藝 15:131-168。 彭雙松。1988。台灣蘭蕙新輯。富蕙圖書出版社。苗栗市。 彭雙松。2002。達摩專輯。富蕙圖書出版社。苗栗市。 傅雪琳、張志勝、何平、歐秀娟、何瓊英。2000。墨蘭根狀莖綠芽分化的研究。華南農業大學學報 21:53-55。 葉秀粦、郭俊彥。1995。墨蘭雌配子體和胚胎發生。熱帶亞熱帶植物學報 3:54-58。 廖曼利。1995。壹、光度及肥料濃度對報歲蘭營養生長與生殖生長的影響 貳、培養基成分、光線及溫度對報歲蘭根莖生長與分化的影響。國立台灣大學園藝學研究所碩士論文。 劉黃碧圓。1995。芭菲爾鞋蘭無菌播種之研究。國立中興大學園藝學研究所碩士論文。 蕭宇倫。2000。培養基成分對報歲蘭‘十八學士’暨其種間雜交種根莖增殖生長與芽體誘導分化之影響。國立台灣大學園藝學研究所碩士論文。 羅英妃。2006。蕙蘭類栽培管理要點。p.43-55。蕙蘭栽培管理手冊。行政院農業委員會動植物防疫檢疫局。台北市。 Arditti, J. 1984. Orchid Biology, reviews and perspectives III. Cornell Press. pp. 432. Arditti, J. and R. L. Knauft. 1969. The effect of auxin, actinomycin D, ethionine, and puromycin on post-pollination behavior of Cymbidium(Orchidaceae)flowers. Amer. J. Bot. 56:620-628. Babb, V. M. and C. H. Haigler. 2001. Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems. Plant Physiology 127:1234-1242. Crafts, C. B. and C. O. Miller. 1974. Detection and identification of cytokinins produced by mycorrhizal fungi. Plant Physiology 54:586-588. Chang, C. and W. C. Chang. 2000a. Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro. Plant Growth Regulation 30:171-175. Chang, C. and W. C. Chang. 2000b. Micropropagation of Cymbidium ensifolium var. misericors through callus-derived rhizomes. In Vitro Cell. Dev. Biol.—Plant 36:517-520. Chang, H. S., D. Chakrabarty, E. J. Hahn, and K. Y. Paek. 2003. Micropropagation of calla lily(Zantedeschia albomaculata)via in vitro shoot tip proliferation. In Vitro Cell. Dev. Biol.—Plant 39:129-134. Chung, J. D., C. K. Chun, and S. O. Choi. 1985a. Asymbiotic germination of Cymbidium ensifolium. II. Effects of several supplements to the medium, pH values and light and/or dark culture periods on rhizome growth and organogenesis from the rhizome. J. Korean Soc. Hort. Sci. 26:186-192. Chung, J. D., C. K. Chun, S. S. Kim, and J. S. Lee. 1985b. Factors affecting rhizome growth and organogenesis of Korean native Cymbidium kanran. J. Korean Soc. Hort. Sci. 26:281-288. Chen, Y., X. Liu, and Y. Liu. 2005. In vitro plant regeneration from the immature seeds of Cymbidium faberi. Plant Cell, Tissue and Organ Culture 81:247-251. Deb, C. R. and Temjensangba. 2006. In vitro propagation of terrestrial orchid, Malaxis khasiana Soland ex. Swartz through immature seed culture. Indian Journal of Experiment Biology. 44:762-766. Gollagunta, V., J. W. Adelberg, J. Rieck, and N. Rajapakse. 2004. Sucrose concentration in liquid media affects soluble carbohydrates, biomass and storage quality of micropropagated hosta. Plant Cell, Tissue and Organ Culture 77:125-131. Gow, W. P., J. T. Chen, and W. C. Chang. 2009. Effects of genotype, light regime, explant position and orientation on direct somatic embryogenesis from leaf explants of Phalaenopsis orchids. Acta Physiol Plant 31:363-369. Hasegawa, A., H. Ohashi, and M. Goi. 1985. Effects of BA, rhizome length, mechanical treatment and liquid shaking culture on the shoot formation from rhizome in Cymbidium faberi Rolfe. Acta Horticulturae 166:25-40. Huang, C. L. and H. Okubo. 2005a. In vitro morphogenesis from rhizomes of Cymbidium sinense. J. Fac. Agr., Kyushu Univ. 50:11-18. Huang, C. L. and H. Okubo. 2005b. Effect of carbon sources in in vitro morphogenesis from rhizomes of Cymbidium sinense. J. Fac. Agr., Kyushu Univ. 50:35-40. Johnson, T. R. and M. E. Kane. 2007. Asymbiotic germination of ornamental Vanda: in vitro germination and development of three hybrids. Plant Cell Tiss Organ Cult. 91:251-261. Ket, N. V., E. J. Hahn, S. Y. Park, D. Chakrabarty, and K. Y. Paek. 2004. Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant. 48:339-344. Kauth, P. J., W. A. Vendrame, and M. E. Kane. 2006. In vitro seed culture and seedling development of Calopogon tuberosus. Plant Cell, Tissue and Organ Culture 85:91-102. Lee, Y. I., C. F. Lu, M. C. Chung, E. C. Yeung, and N. Lee. 2007. Developmental changes in endogenous abscisic acid concentrations and asymbiotic seed germination of a terrestrial orchid, Calanthe tricarinata Lindl. J. Amer. Soc. Hort. Sci. 132:246-252. Lee, Y. I., E. C. Yeung, N. Lee, and M. C. Chung. 2006. Embryo development in the lady’s slipper orchid, Paphiopedilum delenatii, with emphasis on the ultrastructure of the suspensor. Annals of Botany 98:1311-1319. Lee, Y. I., N. Lee, E. C. Yeung, and M. C. Chung. 2005. Embryo development of Cypripedium formosanum in relation to seed germination in vitro. J. Amer. Soc. Hort. Sci. 130:747-753. Miyoshi, K. and M. Mii. 1998. Stimulatory effects of sodium and calcium hypochlorite, pre-chilling and cytokinins on the germination of Cypripedium macranthos seed in vitro. Physiol. Plant. 102:481-486. Nayak, N. R., P. K. Chand, S. P. Rath, and S. N. Patnaik. 1998. Influence of some plant growth regulators on the growth and organogenesis of Cymbidium aloifolium(L.)Sw. seed-derived rhizomes in vitro. In Vitro Cell. Dev. Biol.—Plant 34:185-188. Paek, K. Y. and T. Kozai. 1998. Micropropagation of temperate Cymbidium via rhizome culture. HortTechnology 8:283-288. Paek, K. Y. and E. C. Yeung. 1991. The effects of 1-naphthaleneacetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro. Plant Cell, Tissue and Organ Culture 24:65-71. Pedroza-Manrique, J., C. Fernandez-Lizarazo, and A. Suarez-Silva. 2005. Evaluation of the effect of three growth regulators in the germination of Comparettia falcata seeds under in vitro conditions. In Vitro Cell. Dev. Biol.—Plant 41:838-843. Pedroza-Manrique, J. and Y. Mican-guterrez. 2006. Asymbiotic germination of Odontoglossum gloriosum Rchb. f.(Orchidaceae)under in vitro conditions. In Vitro Cell. Dev. Biol.—Plant 42:543-547. de Paiva Neto, V. B. and W. C. Otoni. 2003. Carbon sources and their osmotic potential in plant tissue culture: does it matter? Scientia Horticulturae 97:193-202. Roy, J. and N. Banerjee. 2002. Rhizome and shoot development during in vitro propagation of Geodorum densiflorum(Lam.)Schltr. Scientia Horticulturae 94:181-192. Shimura, H. and Y. Koda. 2005. Enhanced symbiotic seed germination of Cypripedium macranthos var. rebunense following inoculation after cold treatment. Physiol. Plant. 123:281-287. Shimasaki, K. and S. Uemoto. 1990. Micropropagation of a terrestrial Cymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell, Tissue and Organ Culture 22:237-244. Stewart, S. L. and M. E. Kane. 2006. Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis(Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Organ Cult. 86:147-158. Tomita, M. 1998. Effects of sterilization time, medium composition, and temperature on germination of Calypso bulbosa(L.)Oakes var. bulbosa(Orchidaceae)in vitro. Plant Biotechnology 15:83-86. Van Waes, J. M. and P. C. Debergh. 1986a. Adaptation of the tetrazolium method for testing the seed viability, and scanning electron microscopy study of some Western European orchids. Physiol. Plant. 66:435-442. Van Waes, J. M. and P. C. Debergh. 1986b. In vitro germination of some Western European orchids. Physiol. Plant. 67:253-261. Wotavová-Novotná, K., H. Vejsadová, and P. Kindlmann. 2007. Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species. Biol. Plant. 51:198-200. Yamazaki, J. and K. Miyoshi. 2006. In vitro asymbiotic germination of immature seed and formation of protocorm by Cephalanthera falcate (Orchidaceae). Annals of Botany 98:1197-1206. Zheng, C., X. Zheng, H. Ohno, T. Hara, and S. Matsui. 2005. Involvement of ethylene and gibberellin in the development of rhizomes and rhizome-like shoots in oriental Cymbidium hybrids. J. Japan. Soc. Hort. Sci. 74:306-310.
摘要: 達摩報歲蘭(Cymbidium sinense ‘Da-mo’)的種子發芽率以授粉後170天最好,最佳的發芽率為29.0%。而最低發芽率則在授粉後110天之前,其中授粉後90天的種子發芽率於播種28週後仍為0,可見種子成熟度為影響報歲蘭種子發芽的重要因素。達摩報歲蘭種子以超音波震盪處理60和90分鐘、0.5%次氯酸鈉溶液處理20分鐘或以液體振盪處理30天可明顯增加種子發芽率,其發芽率分別為62.1%和65.5%、64.0%或60.5%,較未經處理者高出2倍。以0.01N NaOH溶液處理者,促進種子發芽的效果較不顯著。而以0.01N KOH溶液或低溫進行預措處理,可能是胚受到傷害,有抑制種子發芽的現象產生。種子播種於較低MS鹽類濃度(1/2MS巨量元素)、蔗糖20 g/L,和不添加任何植物生長調節劑的培養基(經高溫高壓滅菌),其種子發芽率及原球體後續之生長情形較佳,且褐化發生的程度較輕微。如醣類未經高溫高壓者,則以高濃度(40 g/L)的蔗糖和葡萄糖較有助於種子發芽。 根莖中段培植體培養於蔗糖20至40 g/L的培養基中,每個培植體平均可產生2支根莖,且第二分支的形成率達80%,較其他處理濃度佳。而培養於低濃度的蔗糖(低於20 g/L),則無褐化情形產生。未添加活性碳的培養基可些微促進根莖的生長,其所增殖的根莖較長且第二分支的形成率(80%)較高。將根莖頂端培植體培養於BA 5 mg/L+NAA 0.5 mg/L的培養基中,有助於根莖增殖和芽體形成,其中每個月繼代一次,培植體所形成的根莖數(rhizome)和根(root)的數量皆較多。在芽體形成率方面,BA 10 mg/L和NAA 0.5 mg/L組合且每2個月繼代1次者,其芽體形成率高達100%。幼年根莖培植體培養於BA 15 mg/L+NAA 0.5 mg/L的培養基中且每個月繼代一次,有較佳的根莖增殖數,每個培植體可產生將近3支根莖。根莖頂端培植體先於暗環境下培養1個月後在移至16-h光週期環境下,其芽體形成率達80%,每個培植體平均可產生1個具展開葉的芽體,且每個培植體平均至少有7條根形成。培養於完全暗環境下的根莖培植體,會形成白化的過渡芽體,培養於完全光照環境(24-h光照)下時,則會使培植體黃化,且根莖的增殖與芽體和根的形成受到抑制。
Seeds of Cymbidium sinense ‘Da-mo' harvested 170 DAP could reach the highest germination rate. The best germination rate was 29.0%. Minimal germination were observed with seeds harvested before 110 DAP, especially seed germination at 90 DAP were still 0 until investigate over. It could be inferred that the in vitro seed germination of Cymbidium sinense were influenced by seed maturity. Seeds of Cymbidium sinense ‘Da-mo' with ultrasonic treatment for 60 min. and 90 min., 0.5% NaOCl solution for 20 min. or liquid treatment for 30 days could increase the seed germination percentage to 62.1% and 65.5%, 64.0% or 60.5%, double higher than control. No significant improvement on germination was found in 0.01N NaOH solution. Seed germination was inhibited, when seeds pretreatment with 0.01N KOH solution and low temperature treatment. Cultural medium containing low MS salt concentration(1/2MS), sucrose 20 g/L and PGR-free resulted in better germination, growth and lower browning. When media including sugar without autoclaving, high concentration of sucrose and glucose appeared to be better for stimulating germination. Multiplication of rhizomes were better while the rhizome sections were cultured on the media containing sucrose 20 to 40 g/L. Formation of 2nd branches were better while the rhizome sections were cultured on the media without activated charcoal. Culture medium supplemented with BA 5 mg/L+NAA 0.5 mg/L were suitable for the propagation and differentiation of rhizome tip, especially SC1m treatment. The shoot formation from rhizome tips could reach 100% when cultured in SC2m treatment of BA 10 mg/L+NAA 0.5 mg/L. Multiplication of rhizomes were better while the younger rhizome were cultured in SC1m treatment of BA 15 mg/L+NAA 0.5 mg/L medium. Organogenesis of rhizome tips were promoted in dark condition for one month. The shoot formation rate was 80%. White shoot differentiated from rhizome tip if cultured in dark condition. Culture in 24 hours light condition inhibited the proliferation of rhizome and differentiation of shoot and root.
URI: http://hdl.handle.net/11455/29087
其他識別: U0005-2008200900035400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200917211600
Appears in Collections:園藝學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.