Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29095
標題: 化學藥劑處理對蝴蝶蘭Phalaenopsis I-Hsin Cream ’KHM246’裸根苗貯運後品質之影響
Effect of chemicals treatment on quality of bare root Phalaenopsis I-Hsin Cream 'KHM246' after simulated shipment.
作者: 秦子媛
Chin, Tsu-Yuan
關鍵字: Phalaenopsis
蝴蝶蘭
humectants
bare root
simulated shipment
保溼劑
裸根
模擬貯運
出版社: 園藝學系所
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摘要: 本實驗目的旨在探討蝴蝶蘭Phalaenopsis I-Hsin Cream ‘KHM246’裸根貯運過程中,根部失水造成之生理影響,並應用目前化妝品工業中廣泛使用之凝膠劑(Gel,G)與蔗糖酯(Sugar ester, SE),並搭配海藻糖(Trehalose,Tre)、甘油(Glycerol, Gly)、山梨糖醇(Sorbitol,Sor)等具調節組織滲透之化學藥劑,於貯運前將根部浸漬處理,期減輕貯運過程中的失水逆境,使植株受損情形降低,到貨後迅速恢復根部生長活力。 蝴蝶蘭Phalaenopsis I-Hsin Cream ‘KHM246’苗株裸根後以不同保濕藥劑浸漬處理後進行模擬貯運,第一階段實驗以3%山梨糖醇、2%甘油及5%海藻糖搭配凝膠及蔗糖酯兩種不同類型之基底,結果顯示根部活力以甘油-酯(SE-Gly)組1.13 O.D/g最高。經復種後,除對照組外其餘各組根部活力顯著提升。總葉綠素含量最高的是甘油凝膠(G-Gly)組0.66 mg/g,對照組植株下位葉皆呈現黃化,甘油-凝膠(G-Gly)、山梨糖醇-酯(SE-Sor)與甘油-酯(SE-Gly)等組別下位葉黃化發生較少。植株鮮重在貯藏期間海藻糖-酯(SE-Tre)組變化量14.8%最少,並在復種後以山梨糖醇-凝膠(G-Sor)、甘油-凝膠(G-Gly)鮮重增加25.4%、27%較佳。海藻糖的處理對於蝴蝶蘭根部逆境之恢復效果較不佳,而3%山梨糖醇處理對根部造成滲透逆境,需要降低濃度。 第二階段試驗以凝膠配合山梨糖醇1.5%(G-Sor 1.5%)、3.0%(G-Sor 3.0%)、甘油2.0%(G-Gly 2.0%)、甘油4%(G-Gly 4.0%)為添加物。實驗結果顯示G-Gly 2.0%處理於復種1週、2週之葉綠素維持狀況最佳。復種後G-Sor 1.5%與凝膠-甘油2.0%處理之根部活力與新根生長狀況較佳,對照組根部活力最差,下位葉黃化較為嚴重,顯示施用保濕藥劑提高根部活力能減少葉片黃化並使植株提早恢復生育。但較高濃度之山梨糖醇與甘油處理可能對植株造成滲透逆境,造成負擔而無法達到保濕之效果。
The aim of this study was focus on the Pahleanopsis I-Hsin Cream ‘KHM246’ physiology effect caused by water deficient of root during bare-root transportation, and expected using chemical pre-treatment to alleviate the effect of water stress and to reduce the injury, so that the plantlets could recover root vigor as soon as re-plant it . Gel and sugar ester were two types of moistrum that generally using in cosmetic industry these days. We selected these two moistrum bases and add chemicals which like trehalose、glycerol and sorbitols that regulated osmotic potential of tissue, and soaking the roots into chemicals before simulated transportation. Bare-root Phalaenopsis I-Hsin Cream ‘KHM246’pre-treat with different moistrum as below in the first section , 3% sorbitol (Sor)、2% glycerol(Gly)、5% trehalose(Tre) each added into gel(G) and sugar ester(SE) respectively. The root activity result shows SE-Gly 1.13 O.D/g was the highest, and after re-plant the root activity was improved effectively in other treatment except for the control. G-Gly treatment has the highest total chlorophyll content (0.66 mg/g),and basal leaf become yellowing in all control plant. The yellowing appears in bottom leaf was improved within G-Gly、SE-Sor and SE-Gly those treatments. The least fresh weight changing rate was in SE-Tre treatment during storage (14.8%), and the fresh weight increasing rate shows higher in both G-Sor(25.4%) and G-Gly(27%) treatment while re-plant. The result shows that trehalose didn’t improve the growth vigor of Phal. root after transportation, and it seems that 3% sorbitol concentration was a osmotic stress toward the root. Using 1.5%、3.0% sorbitol and 2%、4% glycerol in gel base as pre-treatment chemicals in section two. The result indicate the G-Gly 2.0% treatment with the best chlorophyll maintenance ability during re-planting. G-Sor 1.5% and G-Gly 2.0% treatment could improve root activity、produce more new roots and reveal the flower stalk earlier. All the chemical treatments could improve root activity and reduce yellowing situation better than the control treatment. Those result indicate that Phalaenopsis I-Hsin Cream ‘KHM246’ plantlets with well root activity the vigor recovery was better. Higher concentration of chemicals may lead osmotic stress in plant’s root.
URI: http://hdl.handle.net/11455/29095
其他識別: U0005-2108200911182400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2108200922144500
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