Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29136
標題: 扇形文心蘭試管內開花授粉及與劍葉文心蘭屬間雜交之研究
The studies of pollination, flowering of in vitro plant of Erycina pusilla and intergeneric crosses with Tolumnia cultivars
作者: 邱翊恬
Chiu, Yi-Tien
關鍵字: Erycina pusilla
扇形文心蘭
in vitro flower
intergeneric crosses
試管花
屬間雜交
出版社: 園藝學系所
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摘要: 本論文研究扇形文心蘭試管內無菌株之授粉受精與種子發育,試管內幼苗的發育及開花,以發展可使用於觀賞用途的試管內開花的蘭花。並將扇形文心蘭與劍葉文心蘭進行雜交育種,發展克服屬間雜交之種子發芽障礙的方法,以新花色為育種目標,未來將培育可於試管內開花的新品種。 扇形文心蘭的試管內開花株,經人工自花授粉皆可著果,於授粉後4天花粉可於柱頭發芽,授粉後21天可見胚囊內合子形成。於授粉後10-14週的蒴果成熟度最適合進行無菌播種,種子發芽率為50%。在本試驗的培養方法中,試管內的扇形文心蘭植株需經歷二段停滯期方可順利開花,分別為花莖形成期(播種後6個月)和花蕾形成時期(播種後9個月)。順利開放的小花,具有正常的花器構造及功能。播種後12個月的植株可觀察到兩類的花莖,一種為花芽無法正常發育的小花莖,形成於植株短縮莖第3與第4節,另一種為可形成花蕾的花莖,由短縮莖上第5節以上形成。 劍葉文心蘭自交及種間雜交的結果顯示劍葉文心蘭具自交弱勢或自交不親合性,但花粉均具有發芽能力,授粉後也可著果並形成正常種子,因此不具授粉障礙,可作為育種親本。然而扇形文心蘭和劍葉文心蘭為不同屬的文心蘭亞族成員,且染色體數差異大,可能具有雜交障礙。經由扇形文心蘭和劍葉文心蘭屬間雜交,結果顯示正反交皆可著果,但以劍葉文心蘭為母本時,可形成較多正常種子,種子發芽能力也較高。於培養基中添加活性碳會減少屬間雜交種子發芽,而添加NAA (1-Naphthaleneacetic acid)則可少量增加種子發芽數,且以液體培養基播種較固體培養基有較多的種子發芽。 本研究已建立試扇形文心蘭管內植株繁殖方法與開花習性調查,也可以與異屬蘭花進行屬間雜交而產生子代,未來期望能培育出紅色系的試管內開花植株,並同時進行試管內外選種,以發展新興試管內觀賞性蘭花產品,和培育文心蘭迷你型盆花新品種。
The in vitro pollination, flowering and breeding technologies of Erycina pusilla were developed and established in this thesis. The purposes of all experiments would focus on cultivation new flower-color cultivar besides yellow flower and improve the propagation and flowering procedure for in vitro flowering plant product. The in vitro flowering plants of Erycina pusilla were hand pollinated and fruit set. The pollen tubes were observed on the stigma at 4 days after pollination (DAP), the zygote was observed in embryo sac at 21 DAP. Ten to fourteen weeks after pollination (WAP) was the most appropriate sowing time with germination rate about 50%. Two vegetative growth retardant periods were recorded during the seedling culture, one at 6 MAS (month after sowing) of the floral stalk initiate and two at 9 MAS with the floral bud visible. The 12 month-old in vitro plants would form abnormal and normal inflorescence. Abnormal inflorescence develops retardant and can't form normal floral bud. Normal inflorescence bears visible floral bud and initiate new flower primordium continued. Three Tolumnia cultivars were selected as the parents for intergeneric crosses with Erycina pusilla. The results of self-pollination indicated that Erycina pusilla is self-compatibility, Tol. Gentling Angel and Tol. Rich Orange are also self-compatibility with inbreeding depression, and Tol. Snow Fairy is self-incompatibility. The results of reciprocal crosses between Erycina pusilla and Tol. cultivars were different. The crosses fruit not only bore few seeds but also seed germinated poor. The seed germination number increases slightly when the medium supplement with 0.01-0.1 mg/l NAA (1-Naphthaleneacetic acid). In the future, the seed germination barrier of intergeneric cross must be overcome for enhancing the efficiency of breeding. Selection of red flower characteristic plant would be carried out both in vitro and in vivo seedling cultivation condition.
URI: http://hdl.handle.net/11455/29136
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