請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/29210
標題: 利用RAPD與ISSR分子標誌評估印度棗品種間之遺傳歧異度
Assessment of Genetic Diversity among Indian Jujube Cultivars by Rapd and ISSR Markers.
作者: 蘇慧珊
Su, Hui-Shan
關鍵字: Indian jujube
印度棗
molecular makers
分子標誌
出版社: 園藝學系
摘要: 本研究以RAPD (Random Amplified Polymorphism DNA)及ISSR (inter simple sequence repeat)分子標誌進行15個印度棗(Zizyphus mauritiana Lam.)栽培品種間遺傳相似度之分析與比較其鑑別能力。 以RAPD總條帶分析來看,所求得的平均相似度係數為0.776。其中‘台農1號’與‘高朗1號’之相似度0.949為最高。以RAPD多型性條帶分析來看,所求得的平均相似度係數為0.620。其中‘台農1號’與‘高朗1號’相似度為0.92。 以ISSR總條帶分析來看,所求得的平均相似度係數為0.834。其中‘台農1號’與‘天蜜’之相似度0.97為最高。以ISSR多型性條帶分析來看,所求得的平均相似度係數為0.656。其中‘黃冠’與‘新世紀’之相似度0.91為最高。 由RAPD及ISSR分析中,可供鑑別印度棗品種之特定標誌片段於RAPD方面,獲得7個品種之專屬標誌片段,分別為‘泰國’(OPB-18 / 450bp;OPC-04 / 450bp;OPC-16 / 590bp;OPF-04 / 690bp;OPC-19 / 230bp;OPE-16 / 800bp)、‘新蜜棗’(OPE-17 / 800bp)、‘金鈴’(OPC-06 / 460bp;OPH-08 / 290bp)、‘天蜜’(OPB-10 / 810bp;OPH-12 / 690bp) 、‘翠蜜’(OPF-04 / 250bp和1300bp)、‘特龍’(OPX-08 / 680bp)、及‘黃冠’(OPX-08 / 300bp)。於ISSR方面,獲得3個品種之專屬標誌片段,分別為‘金鈴’(UBC-824 / 290bp;UBC-857 / 280bp)、‘泰國’(UBC-856 / 210bp ;UBC-891 / 530bp)及‘新世紀’(UBC-904 / 1300bp)。 RAPD多型性條帶與總條帶數的比值為0.74,ISSR的多形性條帶與總條帶數的比值為0.63。
Assessment of genetic diversity among Indian jujube(Zizyphus mauritiana Lam.) cultivars by RAPD and ISSR markers was conducted in this study. The average similarity coefficient by RAPD analysis was 0.776 based on total amplified DNA fragments. The highest similarity coefficient was 0.949 between ‘Tainung No.1’ and ‘Kaolang 1’. The average similarity coefficient was 0.620 based on the similarity matrix of RAPD polymorphic markers. The similarity coefficient between ‘Tainung No.1’ and ‘Kaolang 1’ was 0.920. The average similarity coefficient was 0.834 based on the similarity matrix of ISSR total amplified DNA fragments. The highest similarity coefficient was 0.97 between ‘Tainung No.1’ and ‘Tian mi’. The average similarity coefficient was 0.656 based on the similarity matrix of ISSR polymorphic markers. The highest one was 0.91 between ‘Huang guan’ and ‘Xinshiji’. Identification markers generated by RAPD included ‘Thailand’(OPB-18 / 450bp;OPC-04 / 450bp;OPC-16 / 590bp;OPF-04 / 690bp;OPC-19 / 230bp;OPE-16 / 800bp), ‘Xin mi’ (OPE-17 / 800bp), ‘Jin ling’ (OPC-06 / 460bp;OPH-08 / 290bp), ‘Tian mi’ (OPB-10 / 810bp;OPH-12 / 690bp), ‘Cui mi’ (OPF-04 / 250bp and 1300bp), ‘Te long’ (OPX-08 / 680bp), and ‘Huang guan’ (OPX-08 / 300bp). Identification markers generated by ISSR were ‘Thailand’ (UBC-856 / 210bp ;UBC-891 / 530bp), ‘Jin ling’ (UBC-824 / 290bp;UBC-857 / 280bp), and ‘Xinshiji’ (UBC-904 / 1300bp)。 The ratios of polymorphic DNA fragments produced by RAPD and ISSR anaysis were 74﹪and 63﹪, respectively.
URI: http://hdl.handle.net/11455/29210
顯示於類別:園藝學系

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