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Genetic Diversity in Colocasia and Xanthosoma Based on Morphological Traits, Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) Markers
Min, Tee See
|摘要:||搜集台灣及6個其他地區芋種原，包括Colocasia屬30個樣品(KCC)和Xanthosoma屬14個樣品(KCX)，利用外觀形態、RAPD (randomly amplified polymorphism DNA)和SSR (simple sequence repeat)三種方法進行其遺傳岐異度之研究。以28項地上部性狀、9項地下部性狀及2項指標性指數所得資料進行UPGMA群叢分析，可將44個樣品依屬區分成2群組，遺傳相似度為0.32。相似度為0.45之30個KCC樣品可細分成3亞群，KCC175以0.57之相似度獨立成群，第二亞群由14個樣品組成，相似度為0.52，此群內包括葉脈為淺白色及綠色，母芋肉紋色為白色且細少者，其餘15個樣品組成相似度為0.57之第三亞群，葉脈為紫色，肉紋為紫色且較粗的大部分樣品歸於此群內。14個KCX樣品之遺傳相似度在0.66以上。
Abstract The genetic diversity of 30 Colocasia (KCC) and 14 Xanthosoma (KCX) accessions collected from Taiwan and other places were evaluated by morphological traits, RAPD (randomly amplified polymorphic DNA) and SSR (simple sequence repeats). Data for 28 characters of shoot, 9 characters of corm and 2 indexes were subjected to a genetic diversity analysis and the UPGMA cluster analysis was performed. The 44 accessions were clustered into 2 groups according to genus with genetic similarity of 0.32. The Colocasia accessions were clustered into 3 subgroups. KCC175 was independence from other accessions with 0.57 similarities. The second subgroup was composed of 14 accessions with the genetic similarity of 0.52 which included accessions with whitish or green leaf vein, corm flesh with slightly fibrous and white in color. The others comprised the third subgroup with the genetic similarity beyond 0.66. Most of the accessions with purple leaf vein, corm flesh with very fibrous and purple in color were included in this group. A total of 221 RAPD bands, which a means of 10.4 band by each primer, were generated using 15 out of 88 primers in the RAPD analysis. The polymorphism was 98.2%. All accessions were clustered into 2 groups in the RAPD analysis. The similarity within KCX accessions was 0.85 compared to 0.71 within KCC accessions. All KCX accessions except KCX002 were indistinguishable. All KCC accessions were clustered into 8 subgroups. KCC131 and 132 were in the first subgroup with genetic similarity 0.71. KCC002, 004, 062 and 064 were the only accession in subgroup with 0.77, 0.81, 0.78 and 0.84 similarities respectively. The 4th subgroup consisted of KCC029 and 050 which similarity was 0.85. The 6th subgroup included KCC145, 169 and CHC01 with similarity 0.84. The largest subgroup included other 19 accessions with similarity 0.85. In analyzing KCC accessions from SSR results, a total of 73 bands were generated using 13 out of 49 primer pairs. The means of bands generated by a primer pairs was 5.2 and the polymorphism was 93.2%. Thirty accessions were clustered into 5 groups with similarity 0.72. The similarity of the first subgroup included KCC029, 050 and 062 was 0.74. KCC131 and 132 comprised the second group with similarity 0.96. The third group consisted of 6 accessions that had similarity 0.78 and the forth group consisted of KCC002, 004 and 064 with similarity 0.74. The similarity of the fifth group that composed of other 16 accessions had 0.72 similarity. Taro germplasm could be identified by morphology such as leaf blade margin color, leaf vein color, color of corm flesh, flesh fibre and number of cormels. The molecular markers could be used to analyze the accessions within groups. The genetic diversity of the 44 taro accessions could be identified by at least 2 RAPD primers or 4 SSR primer pairs.
|Appears in Collections:||園藝學系|
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