Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29257
標題: 三種蕓苔屬蔬菜熱馴化與耐熱性之研究
The studies on Heat Acclimation and Heat Tolerance in Three sica Vegetable crops
作者: 徐玲明
Ming, Shu Ling
關鍵字: Vegetable
蔬菜
Heat acclimation
Conductivity
Heat tolerance
熱馴化
電導度
耐熱性
出版社: 園藝學系
摘要: 本試驗以熱馴化處理配合電導度測定法,篩選本省芥藍、小白菜及芥菜品 系間之熱馴化能力與耐熱性之差異,調查熱馴化處理前後對高溫之適應性 及生育影響,並探討熱馴化處理對其生理及生化之影響。試驗結果顯示, 利用熱馴化處理(37℃,24小時)後,再置於50℃所估算之熱致死時間( HKT),可有效的區別種源中之耐熱性。14個芥藍品系中最耐熱品系為大心 芥藍 (HKT=40分鐘);30個小白菜品系中最耐熱品系為台農1號(HKT=31分 鐘); 22個芥菜品系中最耐熱品系為亞蔬B544(HKT=42分鐘)。逢機選取10 個芥藍品系,20個小白菜品系及10個芥菜品系,經熱馴化處理(37℃,24 小時)後,置於40℃高溫三天後,再移至溫室生長至成熟期,其地上部相 對鮮物重及乾物重,均顯示與其 HKT值呈正相關。經熱馴化處理1天後, 在40℃生長 9天的情形,耐熱品系生長量沒有變化、或在高溫三天後有恢 復生長、或小幅度生長減少等現象,但不耐熱品系的所有測試項目均顯著 減少(50%∼80%)。於32/27℃生長六個耐熱品系均持續生長,迨 25天後 生長量皆增加,但六個不耐熱品系均生長受阻,25天後之生長量顯著減少 。耐熱品系蔬菜在24小時之熱馴化期間,光合作用及水分潛勢減少的幅度 ,均較不耐熱品系低,且於去馴化 (25/20℃)期間皆能快恢復其光合作用 及水分潛勢。熱馴化處理後,耐熱品系之氮同化作用有加速合成之趨勢, 不耐熱品系有阻礙蛋白質合成,造成氮中間代謝物質累積之現象。
The objectives of this study are to evaluated heat tolerance of Chinese kale (Brassica oleracea L. var. alboglabra)、Pai-tsai( B. campestris L. var. chinensis) and mustard (B. juncea (L.) Czerniak. et Coss) by measuring electrolyte leakage of leaf tissue with conductivity method and to explored the physiological changes during 24hr of teat acclimation at 37℃. Results show the conductivity method could be significant distinguish heat tolerance in three species of Brassica crops after heat acclimation at 37℃ for 24hr; the abilities of heat tolerance could be expressed by heat killing time (HKT) at 50℃. With two hea6t tolerance and heat intolerance lines each of three vegetables crops under high temperture conditions were studies to adaptability of heat stress. After heat acclimation at (37/37℃) for 24hr and followed a continue high temperaure (40/40℃) for 9 days, the heat tolerance lines show a litter reduce in growth rate, or the same, but the heat intolerance lines significant reduce 50%∼80%. Significant positive correlation was found between HKT estimated by conductivity and relative fresh weight or relative dry weight of three Brassica vegetable crops. When the plant were grown at 32/27℃ till harvest, significant increases in the growth rate of heat tolerance lines in three Brassica vegetable crops were found. There were litte or small fractions in the water potential and photosynthesis rate in heat tolerance lines of three Brassica vegetable crops during 24 hr of heat acclimation,then recovered quickly upon deacclimation at 25/25℃. After 24hr heat acclimation, heat tolerance line in three Brassica vegetable crops, show significant increases in the amounts of soluble protein, free amino acid, nitrate and the activity of peroxidase. While the activities of nitrate reductase and glutamine synthetase maintain constant levels, the amounts of ammonia and the activity of acid phosphatase are decrease.
URI: http://hdl.handle.net/11455/29257
Appears in Collections:園藝學系

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