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Interspecific Hybridization between Kalanchoe garambiensis Kudo. and K. blossfeldiana 'Isabella'
Kalanchoe garambiensis Kudo
The pollen viability and pollination between Kalanchoe blossfeldiana ‘Isabella', K. garambiensis Kudo. or K. garambiensis var.‘Purple' were tested for clarifying the behavior of seed set. Furthermore, good seedlings in offsprings population were selected. The optimum sucrose concentration in medium for K. garambiensis pollen germination was 5%-10%, and the pollen germination rate was between 68%-72%. The medium containing 10% sucrose was suitable germination of K. blossfeldiana ‘Isabella' and the pollen germination rate was 48%. The optimum temperature of pollen germination of K. blossfeldiana ‘Isabella', K. garambiensis Kudo. and K. garambiensis var.‘Purple' was 25℃. However, under the temperature of 15℃, the pollen germination rate was 1.5%-5%. Trying to confer the pollination and seed production of three parents by the methods of hand selfing, outcrossing, selfing, open pollination and covering with net after emasculation was conducted. For K. blossfeldiana ‘Isabella', K. garambiensis and K. garambiensis var.‘Purple'as hand selfing and open pollination were able to produce more seeds. In contrast, selfing produced less seeds. And, covering with net after emasculation was unable to produce seeds. When pollens of K. blossfeldiana ‘Isabella' were applied to the stigma of K. garambiensis, pollen tubes penetrated the stigma into the ovules within two days after pollination. In reciprocal pollination, pollen germination was poor on the stigma, and pollen tubes with abnormal morphology were arrested in the styles. When K. garambiensis or K. garambiensis var.‘Purple' to be a seed parent was able to produce more seeds, the mature seeds per capsule were 143 or 133 as well as K. blossfeldiana ‘Isabella'was a seed parent and crossed with K. garambiensis or K. garambiensis var.‘Purple', mature seeds per capsule were 31 or 36. In the crosses of K. garambiensis × K. blossfeldiana ‘Isabella' and K. garambiensis var.‘Purple'× K. blossfeldiana ‘Isabella', 7 plants in 430 offspring seedlings population were selected.
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