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Studies on Shoot Tip Culture of Loquat (Eriobotrya japonica Lindl.) in Vitro
Shoot tip culture
|摘要:||本研究採用台灣之主要栽培枇杷之茂木品種(Eriobotrya japonica Lindl. cv. Mogi)為試驗材料，探討培養基組成及培養方法對莖頂培養成活之影響，以求建立一可行之微體繁殖方法。
Shoot tip culture of the loquat (Eriobotrya japonica Lindl.)was studied, the optimal micropropagation condieions for culture establishment,shoot proliferation and shoot rooting were investigated. Shoot tip were harvested about 5cm in length from summer shoots and shoot apices excised 0.5mm for initial culture.The best grown was achieved when shoot apices cultured on solid medium with 1/2 strength of Mrashige and Skoog (MS) containing BA 1 mg/l,sucrose 30 g/l and agar 7 g/l for 30 days.These explants were transferred to the same component solid medium for further growth. Multiple shoot were induced by MS medium containing BA 4 mg/l, sucrose 30 g/l and agar 8 g/l for 30 days.For elongation of these young shoots,MS medium supplemented with BA 2 mg/l,sucrose 10 g/l and agar 8 g/l or surcose 30 g/l and agar 8 g/l plus kinetin 0.5 mg/l gave a good response. For rooting, the shots grew above 20 mm in hight , were placed on 1/2 MS medium with IBA 10 mg/l , surcose 20 g/l and agar 8 g/l and incubated in darkness for 7 days , then transplanted to an auxin-free 1/2 MS medium for 23 days.
|Appears in Collections:||園藝學系|
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