Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29377
標題: 三倍體葡萄胚珠培養之研究
Studies of Ovule Culture of Triploid Grape
作者: 蔡孟佑
Tsai, Meng-You
關鍵字: 胚珠培養
ovule culture
三倍體
葡萄
triploid
grape
出版社: 園藝學系所
引用: 參考文獻 王為一。1988。葡萄新品種引進及選拔。葡萄產業研究與發展研討會專集pp. 1-23。 余旦華、張磊、薛菊萍、趙改榮。1992。早熟和四倍體胚培養的研究。果樹科學1: 1-7。 尚忠海。2007。巨峰葡萄胚培养技術的研究。河南科學 25:405-408。 李桂榮、王躍進、唐冬梅、駱強偉。2004。胚挽救無核葡萄新品種取樣時期的研究。河北農業大學學報 27:17-21。 周香豔、張甯、王旺田、王翠玲、吳兵。2012。流式細胞術檢測4種提取沙棘核DNA方法的比較。甘肅農業大學學報 4:155-160。 林禎祥。2003。夜來香組培苗發根、田間植株性狀表現及核型分析之研究。國立嘉義大學園藝學系碩士論文。 武季玲、李勝、薑寒玉、高育紅、牛俊義。麻黃屬植物染色體制片技術及核型分析研究。中國中藥雜誌 34:2725-2729。 徐海英、閻愛玲、張國軍。2001。葡萄二倍體與四倍體品種間雜交胚珠的離體培養。果樹學報 18:317-320。 許怡萱。2010。無子葡萄選育技術之研究。國立中興大學園藝學系碩士論文。 農業統計年報。2011。行政院農業委員會。 張致盛、張林仁、林嘉興。2004。台灣葡萄生產產期調節技術。葡萄栽培技術研討會專集 pp. 37-53。 張致盛。2006。台灣葡萄產業分析。台中區農業專訊 42:14-17。 郭印山、郭修武、張海娥、李軼暉、李成祥。2005。利用胚挽救技術獲得三倍體葡萄植株研究。瀋陽農業大學學報 36: 606-608。 郭修武、郭印山、張海娥、李軼暉、李成祥。2007。接種時期和培養基對無核葡萄胚挽救的影響。園藝學報34:329-332。 賀普超。1999。葡萄學。中國農業出版社。 廖勇為。1987。葡萄。落葉果樹分類學。五洲出版社。pp. 171-191。 趙勝建、郭紫娟。2004。三倍體無核葡萄育種研究進展。果樹學報 21:360-364。 趙艷華、李春敏、吳雅琴。2003。植物的胚搶救。河北農業科學 7:55-58。 劉海琳、尚忠海、崔玲華、廖康、陳力耕。2006。葡萄幼胚培養技術的研究(II)接種時間及處理方法對胚培養的影響。河南科學 24: 844 -848。 岡本五郎、平野健、田中丸直美、大森直樹。1992。2倍体と4倍体のマスカットブドウの正逆交雑種子の発育と実生の獲得。岡山大学学報80:85-92。 Abdel-Mawgoud, A. M. R., A. S. Tantaway, M. M. Hafez and H. A. M. Habib. 2010. Seaweed extract improves growth, yield and quality of different watermelon hybrids. Research Journal of Agriculture and Biological Sciences 6:161-168. Adaniya, S., and D. Shirai. 2001. In vitro induction of tetraploid ginger(Zingiber officinale Roscoe) and its pollen fertility and germinability. Scientia Horticulturae 88:277-287. Anderson, N., D. H. Byrne, J. Sinclair and A. Millie Burrell. 2002. Cooler temperature during germination improves the survival of embryo cultured peach seed. HortScience 37:402-403. Burger, P. and P. G. Goussard. 1996. In vitro culture of ovules and embryos from seedless grapes (Vitis vinifera L.). South African Journal of Enology and Viticulture 17:31-37. Coombe, B. G. 1976. The development of fleshy fruit. Annual Review of Plant Physiology 27:507-528. Dantas, A. C. M., J. I. Boneti, R. O. Nodari and M. P. Guerra. 2006. Embryo rescue from interspecific crosses in apple rootstocks. Pesquisa Agropecuaria Brasileira 6:969-973. David, W. G. 2010. Flow cytometry and fluorescence-activated cell sorting in plants: the past, present, and future. Biomedica 30:65-70. Ebadi, A., J. E. Moghadam and R. Fatahi. 2009. Evaluation of 22 populations achieved from controlled crossing between some seeded seedless grapevine cultivars. Scientia Horticulturae 119:371-376. Ebadi, A., P. May and B. Coombe. 1996. Effect of short-term temperature and shading on fruit-set, seed and berry development in model vines of Vitis vinifera, cvs Chardonnay and Shiraz. Australian Journal of Grape and Wine Research 2:2-9. Emershad, R. L. and D. W. Ramming. 1984. In ovulo embryo culture of vitis vinifera. c.v.‘Thompson seedless’. HortScience 71:873-877. Fathi, H. and U. Jahan. 2012. Review of embryo culture in fruit trees. Annals of Biological Research 3:4276-4281. FAO. 2010. Food and Agriculture Organization. http://www.fao.org/index_en.htm Fernandez, G. E., J. R. Clark and J. N. Moore. 1991. Effect of seedcoat manipulation on the germinaton of stenospermocarpic grape embryos cultured in ovule. HortScience 26:1200. Galbraith, D. W., K. R. Harkins and J. M. Maddox. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissue. Science 220:1049-1051. Gamborg, O. L., R. A. Miller and K. Ojima. 1968. Nutrient requirement of suspension cultures of soybean root cell. Experimental Cell Research 50:151-158. Gray, D. J., J. A. Mortensen and C. M. Benton. 1990. Ovule culture to obtain progeny from hybrid seedless bunch grapes. Journal of the American Society for Horticultural Science 115:1019-1024. Grosser, J. W., J. Jiang, F. A. A. Mourao-Fo, E. S. Louzada, J. L. Chandler and F. G. Gmitter. 1998. Somatic hybridization, an integral component of citrus cultivar improvement: I. Scion improvement. HortScience 33:1057-1059. Gudin, S., L. Arene, A. Chavagnat and C. Bulard. 1990. Influence of endocarp thickness on rose achene germination: genetic and enviromental factors. Hortscience 25:786-788. Heller, F. O. 1973. DNS-Bestimmung an Keimwurzeln von Vicia faba L. mit Hilfe der Impulscytophotometrie. Berichte der Deutschen Botanischen Gesellschaft 86:437-441. Hummer, K. 1999. Biotechnology in plant germplasm acquisition. pp. 25-40. In: Erica B. E. (ed.). Plant Conservation Biotechnology. British Library. Iland, P. G. and R. Ristic. 2005. Relationships between seed and berry development of Vitis Vinifera L. cv Shiraz: Developmental changes in seed morphology and phenolic composition. Australian Journal of Grape and Wine Research 11:43-58. Iwahori, S., R. J. Weaver and R. M. Pool. 1968. Gibberellin-like activity in berries of seeded and seedless tokay grapes. Plant Physiology 43: 333-337. Kumar, A. S. Sharma and D. S. Gill. 2001. Ovulo embryo culture in grapes – a review. Agricultural Reviews 22:127-131. Lawiay, P., E. Tkouanne, J. Lespinasse, J. Regnard and J. Kelner. 1995. Genotypic differences in the axillary bud growth and fruiting pattern of apple fruiting branches over several years- an approach to regulation of fruit bearing. Scientia Horticulturae 64 :265-281. Laves, S. 1960. Effect of gibberellic acid on seeded grape. Nature 185:395. Ledbetter, C. A. and D. W. Ramming. 1989. Seedlessness in grapes. Horticultural Reviews 11:159-184. Liu, S. M., S. R. Sykes and P. R. Clingeleffer. 2003. Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.). Australian Journal of Agricultural Research 54:869-876. Liu, S. M., S. R. Sykes and P. R. Clingeleffer. 2008. Effect of culture medium, genotype, and year of cross on embryo development and recovery from in vitro cultured ovulesin breeding stenospermocarpic seedless grape varieties. Australian Journal of Agricultural Research 59:175-182. Loomis, N. H. and J. H. Weinberger. 1979. Inheritance studies of seedlessness in grape. Journal of the American Society for Horticultural Science 104:181-184. Loureiro, J., E. Rodriguez, J. Dolezel and C. Santos. 2006. Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Annals of Botany 98: 515-527. Mallon, R., J. Rodrıguez-Oubina and M. L. Gonzalez. 2010. In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flowcytometry and RAPD analysis. Plant Cell, Tissue and Organ Culture 101:31-39. McGovern, P. E., 2003. Ancient wine: the search for the origins of viniculture. Princeton University Press, Princeton. Monnier, M. 1995. Culture of zygotic embryos. pp. 117-153. In: Thorpe T. A. (ed). In vitro embryogenesis in plants. Kluwer Academic Publishers. Canada. Murashige, T. and F. Skoog.1962. A revised medium of rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497. NeSmith, D. S. and J. R. Duval. 2001. Fruit set of triploid watermelons as a function of distance from a diploid pollinizer. HortScience 36:60-61. Nitsch, J. 1970. Hormonal factors in growth development. pp.427-472. In: Hulme A.(ed). The Biochemistry of Fruits and Their Products. Harcourt, London. Nitsch, J. P. and C. Nitsch. 1969. Haploid planrs from pollen grains. Science 163:85-87. Noirot, M., P. Barre, C. Duperray , J. Louarn and S. Hamon. 2003. Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree. Annals of Botany 92:259-264. Notsuka, K., T. Tsuru and M. Shiraishi. 2001. Seedless-seedless grape hybridization via In-ovulo embryo culture. Journal of the Japanese Society for Horticultural Science 70:7-15. Owen, C. L. 2008. Grape. pp.197-229. In: Hancock J. F. (ed.). Temperate Fruit Crop Breeding. Springer, London. Perrin, M., D. Martin, D. Joly, G. Demangeat, P. This and J. E. Masson. 2001. Medium-dependent response of grapevine somatic embryogenic cells. Plant Science 161:107-116. Ponce, M. T., M. E. Guinazu and R. Tizio. 2002. Improved in vitro embryo development of stenospermic grape by putrescine. Biocell 26:263-266. Raghavan, V. and P. S. Srivastava, 1982. Embryo culture . pp. 195-230. In : Johri B. M. (ed.). Experimental Embryology of Vascular Plants. Farrar Straus. Ramming, D. W. and R. L. Emershad. 1982. In ovulo embryo culture of seeded and seedless Vitis vinifera. HortScience 17(Abstr):487. Ray, P. K. 2002. Breeding tropical and subtropical fruits. Narosa Publishing House, New Delhi. Reed, S. M. 2005. Embryo rescue. Plant Development and Biotechnology 40:235-239. Rombough, L. 2002. The grape grower. Chelsea Green Publishing. Vermont. Sharma, D. R., R.Kaur and K. Kumar. 1996. Embryo rescue in plants-a review. Euphytica 89:325-337. Soltis, P. S. and D. E. Soltis. 2000. The role of genetic and genomic attributes in the success of polyploids. Proceedings of the National Academy of Sciences of the United States of American 97:7051-7057. Spiegel- Roy, P., N. Sahas, J. Baron and U. Lavi. 1985. In vitro culture and plant formation from grape cultivars with abortive ovules and seeds. Journal of the American Society for Horticultural Science 110:109-112. Sukendah, M. and L. O. Cedo. 2005. In vitro conservation of coconut (Cocos nucifera L.) embryos in culture media. Biotropia 25:11-21. Tiburcio, A. F., J. L. Campos, X. Figueras and R. T. Besford. 1993. Recent advances in the understanding of polyamines function during plant development. Plant Growth Regulation 12: 331-340. Trevor, A. T. 2007. History of Plant Tissue Culture. National Center for Biotechnology Information 37:80-169. Tromp, J. 1997. Maturity of apple cv. Elstar as affected by temperature during a six-week period following bloom. Journal of Horticultural Science 72:811-819. Varoquaux, F., R. Blanvillain, M. Delseny and P. Gallois. 2000. Less is better: new approaches for seedless fruit production. Trends in Biotechnology 18:233-242. Wakana, A., I. Fukudome, N. Hanada, M. Hiramatsu, K. Sakai and K. Kajiwara. 2008. ‘Bea-Kei’, a New Triploid Seedless Grape Cultivar Derived from a `Muscat Bailey A’ × `Kyoho’ Cross. Journal of the Faculty of Agriculture 53:423-427. Wan, Y., H. Schwaninger, D. Li, C. J. Simon, Y. Wang and C. Zhang. 2008. A review of taxonomic research on Chinese wild grapes. Vitis 47:81-88. Wang, W. Q., S. Q. Song, S. H. Li, Y. Y. Gan, J. H. Wu and H. Y. Cheng. 2011. Seed dormancy and germination in Vitis amurensis and its variation. Seed Science Research 21:255-265. Wang, A. L., Y. J. Wang, D. M. Tang, J. X. Zhang and C. H. Zhang. 2010. Research on improvement of seedling rate in embryo rescue of seedless grape. Scientia Agricultura Sinica 43:4238-4245. Yamashita, H., I. Shigehapa and T. Haniuda. 1998. Production oftriploid grapes by in ovulo embryo culture. Vitis 37:113-117. Yang, D., W. Li, S. Li, X. Yang, J. Wu and Z. Cao. 2007. In vitro embryo rescue culture of F1 progenies from crosses between diploid and tetraploid grape varieties. Plant Growth Regul ation 51:63-71. Zabadal, T. J. and M. J. Bukovac. 2006. Effect of CPPU on fruit development of selected seedless and seeded grape cultivars. HortScience 41:154-157. Zhang, W., H. Hao, L. Ma, C. Zhao and X. Yu. 2010. Tetraploid muskmelon alters morphological characteristics and improves fruit quality. Scientia Horticulturae 125:396-400. Zohary, D. and P. Spiegel-Roy. 1975. Beginnings of fruit growing in the old world. Science 187:319-327.
摘要: 本研究以二倍體`貝利A’與四倍體`巨峰’葡萄為材料,測試自交胚珠及相互雜交之胚珠進行離體培養之適當的取胚時間與培養條件,調查不同培養條件下之胚珠萌芽率。 試驗結果顯示`貝利A’與`巨峰’葡萄自交之胚珠於花後42天取出培養皆不能萌芽,而`貝利A’葡萄在花後63天以液態培養基在26±1℃及光周期16/8 小時下培養37天後進行縱切刻傷處理,之後再以固態培養基培養50天後之胚珠萌芽率可達100%,`巨峰’葡萄在相同培養條件下之胚珠萌芽率也可達63.3%。而以`貝利A’葡萄為母本與`巨峰’雜交之胚珠,於花後49天以固態培養基在26±1℃及光周期16/8 小時下培養51天後進行縱切刻傷處理,之後繼續培養50天之胚珠萌芽率為30%。而以`巨峰’葡萄為母本與`貝利A’雜交之胚珠在相同培養條件下之胚珠萌芽率可達60%。 本研究另以CPPU處理授粉後的果穗,調查CPPU處理對後續胚珠培養之影響。以`貝利A’與`巨峰’葡萄雜交授粉後7天之果穗處理1ppm CPPU,結果發現CPPU處理組果實含胚珠百分率為59.7%,而對照組果實含胚珠百分率為31.9%,顯示CPPU處理能增加雜交授粉後含胚珠的果實數目。經CPPU處理之果穗於花後56天進行胚珠培養44天,然後進行縱切刻傷處理,再經50天培養後之萌芽率為40%。 雜交胚珠萌芽後成苗之植株,以流式細胞儀分析其染色體,結果發現以`貝利A’葡萄為母本與`巨峰’雜交授粉後,在花後7周取胚珠培養51天後進行縱切刻傷處理之三倍體植株獲得率較高,為33.3%。
The ovules of self-pollination and reciprocal crosses between diploid `Muscat Bailey A’ and tetraploid `Kyoho’ grapes were harvested at different stages of development. The effects of different in vitro culture conditions on ovule germination rates were investigated. The resultes indicated that the germination rate of self-pollination ovules of `Muscat Bailey A’ and `Kyoho’ grapes were 0% when the ovules were harvested 42 days after blooming. Ovules of `Muscat Bailey A’ grapes were harvested 63 days after blooming and were cultured in liquid medium at 26±1℃with photoperiod 16/8 (light/dark) hours for 37 days before cut longitudinally. The ovules were then cultured in solid medium for another 50 days and the final germination rate was 100%. The ovules of `Kyoho’ grapes were cultured in the same condition and had a germination rate of 63.3%. `Muscat Bailey A’ × `Kyoho’ ovules were harvested 49 days after blooming and were cultured at 26±1℃ with photoperiod 16/8 (light/dark) hours for 51days. The ovules were then cut longitudinally and incubated for another 50 days, and the final germination rate was 30%. Ovules of `Kyoho’ × `Muscat Bailey A’ were cultured in the same culture conditions and had a germination rate of 60%. Another objective of this study was to investigate the effect of CPPU on ovule culture. Clusters of `Muscat Bailey A’ × `Kyoho’ grapes were treated with 1ppm CPPU 7days after pollination and the percentage of berry containing ovules were examined. It was found that 59.7% of the CPPU treated berries had normal ovules and the control had only 31.9% of berries containing normal ovules. It seemed that the CPPU treatment can increase the percentage of fruit containing normal ovules. Ovules of CPPU treated `Muscat Bailey A’ × `Kyoho’ grapes were harvested 56 days after blooming and were cultured for 44 days before cut longitudinally. The ovules were then cultured for additional 50 days and had a final germination rate of 40%. The survied plants were analyzed by flow cytometry and the result indicated that 33.3% triploid plants was obtained from the `Muscat Bailey A’ × `Kyoho’ ovules harvested 49 days after blooming and cut longitudinally after 51 days of culture.
URI: http://hdl.handle.net/11455/29377
其他識別: U0005-2603201308294000
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