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標題: 九重葛之組織培養化學誘變
The Mutation of Bougainvillea in vitro by Mutagents
作者: 范芳綺
Fan, Fang-Chi
關鍵字: 器內培養
in vitro
Sodium azide
出版社: 園藝學系所
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摘要: 本試驗使用九重葛‘Pink Pixie’、‘Pink Pixie Variegate’、‘Bella’及其芽條變異營養系‘Bella M1’,以及中興大學雜交育成的品種‘Pink Panther’作為材料。取莖頂梢為培殖體並以添加BA之MS培養基進行培養,先建立微體繁殖系統,再配合化學誘變劑進行誘變處理。 莖段培養於含有2 mg L-1 BA的MS培養基,‘Pink Pixie’、‘Bella’或‘Bella M1’經8週培養時有較多的增殖率,每培殖體約可增殖倍率分別為7.78、8.53或9.42個。‘Pink Pixie Variegate’ 經8或10週培養具最佳的增殖率,分別為6.83或6.81個。然而‘Pink Panther’莖段培殖體經4週培養有較高的增殖率,每培養體約有4.59個,培養時間較長增殖率反而下降。 不同九重葛品種對化學誘變劑的反應呈現差異。培殖體的致死率及腋芽萌芽率隨著誘變劑處理時間及濃度增加而降低。當以oryzalin處理0.1%-0.3%時,未獲得任何的變異株;‘Pink Pixie Variegate’以0.1%Colchicine溶液處理1天的培殖體獲得1株嵌合變異株‘Pink Pixie Variegate M1’。經再繼代培養分離出穩定的變異株‘Pink Pixie Variegate M2’。此植株經微體繁殖成營養系後,並以UPOV規範完成DUS的檢定。另外‘Pink Pixie’經0.25 mM疊氮化鈉溶液處理1天後也獲得2株花型態的變異株。
In this research, bougainvillea cutivars:‘Pink Pixie’、‘Pink Pixie Variegate’、‘Bella’、‘Bella M1’ and ‘Pink Panther’ were used as materials. Shoot explants were cultured on the MS medium with BA. A micropropagation system was established first from bougainvillea, then mutagents were added into the medium for inducing mutation. Shoot explants were cultured in full strength MS medium supplemented with 2 mg L-1 BA. After 8 weeks, the multiplication rate of ‘Pink Pixie’, ‘Bella’ or ‘Bella M1’ was 7.78, 8.53 or 9.42 respectively. ‘Pink Pixie Variegate’ was cultured for 8 or 10 weeks, the multiplication rate was 6.83 or 6.81. However ‘Pink Panther’ cultured for 4 weeks had a higher multiplication rate, it was 4.59. The longer culture the multiplication rate was the loss explant. The mutagent response of bougainvillea was depend on cultivars. Treat with higher concentration and longer exposure time of mutagents, the percentage of survival on lateral shoot sprouting was decreased. Explants treated with Oryzalin at 0.1%-0.3%, did not get any mutants. ‘Pink Pixie Variegate’ treated with Colchicine of 0.1% for 1 day, proliferated a chimeric mutant ‘Pink Pixie Variegate M1’. The mutant ‘Pink Pixie Variegate M2’ were separated after several subcultures. This plant through micropropagation to establish a clone. And then following the UPOV guidelines the clone was tested its DUS. In addition, ''Pink Pixie'' treated with NaN3 of 0.25 mM for 1 day, obtained two flower-off-types mutants.
其他識別: U0005-2507201213142100
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