Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29415
標題: 添加α-胺基異丁酸、活性碳、硝酸鉀對仙履蘭內花芽發育及光源、硫胺素對出瓶苗生長之影響
Effect of α-Aminoisobutyric acid, Activated Charcoal, KNO3 on Flower Bud Development in vitro and Light Source, Thiamine on Plants Growth of Paphiopedilum
作者: 楊采凌
Yang, Tsai-Ling
關鍵字: 仙履蘭
Paphiopedilum
α-胺基異丁酸
發根
硝酸鉀
活性碳
發光二極體
硫胺素
α-Aminoisobutyric acid
Rooting
Activated charcoal
LED
Thiamine
出版社: 園藝學系所
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摘要: 摘要 大部分從仙履蘭植株所得之培植體在組織培養上易因受傷乙烯以及培養組成分的影響而導致組織的褐化或壞死。本試驗主要探討培(i)培養基中添加乙烯生合成抑制劑α-胺基異丁酸(α-Aminoisobutyric acid, AIB)對兩個階段(S1、S3)之斑葉單花‘Maudiae type’仙履蘭子房基部花芽生長及褐化的影響(ii)添加活性碳或補充MS配方硝酸鉀之1/3、2/3、1 × 劑量對Paphiopedilum hybrid ‘97100’植株瓶內根生長的影響。另外,調整外在的生長條件或添加活力劑,例如:不同光源及硫胺素處理,來試驗,對剛出瓶Paphiopedilum hybrid ‘9291’ 、Paphiopedilum venustum×sib ‘7580’ 及Paphiopedilum hybrids ‘9751幼苗生長及品質的影響, 添加乙烯生合成抑制劑AIB,在培養四個月後,除了S1階段花芽在添加200-400 mg∙l-1AIB,仍維持黃綠色及黃色的狀態,其他包括S1及S3階段花芽均大面積褐化或壞死。 仙履蘭Paphiopedilum hybrid‘97100’在含有1 × MS KNO3(1900 mg∙l-1)硝酸鉀,有較多的根,根數為3.57條,並觀察到畸形根的形成。活性碳的添加,促進根的伸長,能正常生長,但無增加根數的效果。 仙履蘭Paphiopedilum hybrid ‘9291’瓶苗出瓶後在四種光源下生長,葉片生長在不同光源下無差異。總葉綠素在藍光處理下含量最高,混和光則最低。根部活性白光處理組最高,可達3.54 OD A480/g F. W.,而在單一紅光或藍光處理組則較低。 硫胺素也被稱之為維他命B1,其影響根生長及植株的發育。使用硫胺素澆灌處理仙履蘭P. venustum × sib‘7580’及Paphiopedilum hybrid 9751’出瓶苗。硫胺素對仙履蘭P. venustum sibב7580’的生長在添加2g∙l-1顯著增加葉長及葉寬,而硫胺素處理可以增加葉綠素含量,其中澆灌0.5g∙l-1硫胺素處理組有最高之葉綠素含量。但在根部活性無顯著促進的效果,處理後45天也無新根長出。而硫胺素處理仙履蘭Paphiopedilum hybrid ‘9751’植株,並無促進生長,但在處理後45天有新根長出。硫胺素各濃度處理皆可促進此品系仙履蘭葉片之葉綠素含量,根部活性則是在1g∙l-1硫胺素處理較高為1.22 OD A480/g F. W.。
Abstract Numerous explants from Paphiopedilum species for tissue culture present greatly tissue browning and necrosis resulted from wounding ethylene and media constituents. The aims of this study were to investigate (i) the effect of α-aminoisobutyric acid, AIB, act as an ethylene biosynthesis inhibitor, on ovary based flower bud excised from two flowering stages (S1and S3) of Paphiopedilum Maudiae type and (ii) the effect of potassium nitrate, KNO3 original MS medium 1/3 KNO3, and 1/3,2/3,1 × supplement and activated charcoal supplementation on root growth of Paphiopedilum hybrid ‘97100’ in vitro plantlets. In addition, the effect of culture conditions such as light spectrum including four light-emitting diode, LED on Paphiopedilum hybrid ‘9291’ and nutrient supplementation thiamine on two Paphiopedilum venustum×sib ‘7580’ and Paphiopedilum hybrid ‘9751’ explants of flask plantlets was examined. In Paphiopedilum Maudiae type, only explants from S1 stage flower bud remained yellow greenish tissues in culture media with 200-400 mg∙l-1 AIB; whereas those explants from S1 and S3 stage became largely browning or necrosis after 4 months. In vitro plantlets of Paphiopedilum hybrid ‘97100’ had more root number (3.57) with abnormal root formation in original culture media containing KNO3 at 1900 mg∙l-1. Addition of activated charcoal promoted plantlet root length and normal root growth but not increased root number. The leaf growth of Paphiopedilum hybrid ‘9291’ explants flask plantlets was no dissimilarity under four LED. The highest total chlorophyll contents of those plantlets were under blue light (3.54 OD A480/g F. W.), the lowest ones were under blue and red light combination LED. Besides, plant which under white light their root activities were the higher than under single red or blue light. Thiamine also known as Vitamin B1 has an effect on root growth and development of new plants. Use of thiamine (2 g∙l-1 ) in Paphiopedilum venustum×sib ‘7580’ explants flask plantlets not only markedly increased leaf length and width, but also improved the chlorophyll contents, particularly irrigated 0.5 g∙l-1 of thiamine had the higher level than the other treatments. However, there was no significantly effect on root activity and no new root formation after transferring in pots for 45 days. In Paphiopedilum hybrid ‘9751’, explants flask plantlets had new root formation but not enhanced their growth after 45 days. In fact, addition of thiamine promoted their chlorophyll contents and root activities, especially irrigated 1 g∙l-1 of thiamine had the higher root activity (1.22 OD A480/g F. W.) than the other treatments.
URI: http://hdl.handle.net/11455/29415
其他識別: U0005-0808201320301300
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0808201320301300
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