請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/29420
標題: 燈籠草屬同節物種或異節物種之種間雜交
The Interspecific Crossing between Sectional Species or Intersectional Species of Kalanchoe
作者: 盧勝鍵
Lu, Sheng-Jian
關鍵字: 燈籠草屬
Kalanchoe
種間雜交
Bryophyllum
interspecific hybridization
出版社: 園藝學系所
引用: 王一霖。2011。鳳仙花屬植物之種間雜交障礙。國立台灣大學園藝學系研究所碩士論文。119 pp.。 王嘉偉。2011。燈籠草屬異節物種間的雜交。國立中興大學園藝系研究所碩士論文。72 pp.。 林天枝、莊杉行、洪澨堂。1991。藥劑處理對促進朝鮮薊抽苔之影響。臺中區農業改良場研究彙報 32: 11-15。 侯宇龍、朱建鏞。2004。鵝鑾鼻燈籠草和長壽花之花粉發芽及其種間雜交之研究。中國園藝50:187-196。 侯宇龍。2003。鵝鑾鼻燈籠草與長壽花之種間雜交育種。國立中興大學園藝系研究所碩士論文。89 pp.。 胡適宜。1990。胚。被子植物胚胎學。p. 207-225。曉園出版社。台北。 高典林。2006。現代作物育種學。467pp.。藝軒圖書出版社。台北。 陳嘉芬。1993。數量遺傳。現代遺傳學。p. 139。藝軒圖書出版社。台北。 陳德順、胡大維。1976。台灣外來觀賞植物名錄。p.303。川流出版社。台北。 黃倉海。2007。台灣原生燈籠草屬物種開花生理、種間雜交與ISSR分子標誌分析之研究。國立中興大學園藝系研究所博士論文。190 pp.。 黃敏展。1998。花卉育種。亞熱帶花卉學總論。中興大學園藝系。台中。367pp.。 楊梨玲。1998。聖誕紅實生育種之研究。國立中興大學園藝學研究所碩士論文。100 pp.。 楊曉苓、楊利平、尚愛芹、劉鳳欒。2009。不同授粉處理對百合花粉萌發生長及坐果率的影響。河北農業大學學報32(2): 46-49。 葉志新、楊雅淨、毛清源、傅仰人、廖芳心。2011。利用ISSR 分子標誌研究長壽花品種之遺傳變異。臺灣園藝57(3):171-181。 褚哲維。2012。台灣原生石竹新品種之開發。國立中興大學園藝系研究所碩士論文。66 pp.。 鄭怡婷。2009。重瓣長壽花之雜交育種。國立中興大學園藝系研究所碩士論文。64 pp.。 盧守耕。1976。數量遺傳。作物育種學導論。p. 67-68。國立台灣大學農學院叢書第16號。國立台灣大學農學院。台北。 魏子耀。2010。夏蓳之花朵性狀遺傳與遠緣雜交障礙。國立台灣大學園藝學系研究所碩士論文。145 pp.。 蘇姬泰。2009。利用疊氮化鈉培養基誘變長壽花。國立中興大學園藝研究所碩士論文。台灣:台中。67 pp.。 Abdel-Raouf, H. S. 2012. Anatomical traits of some species of Kalanchoe (Crassulaceae) and their taxonomic value. Ann. Agric. Sci. http://dx.doi.org/10.1016/j.aoas. 2012.03.002. Aida, R., and M. Shibata. 2002. High frequency of polyploidization in regenerated plants of Kalanchoe blossfeldiana cultivar ‘Tetra Vulcan’. Plant Biotech. 19: 329–334. Akulova-Barlow, Z. 2009. Kalanchoe. Cactus and Succulent Journal. 81(6): 268-276. Bailey, L. H. and E. Z. Bailey. 1976. Kalanchoe. p.620-623. In: A Concise Dictionary of Plants Cultivated in the United State and Canada, Hortus Third. Macmillan Publishing Co., New York, USA. Baker, J. G. 1881. Kitchingia. J. Linn. Soc. Bot. 18: 268. Basnizki, J. and E. E. Goldschmidt. 1994. Further examination of gibberellin A3 effectson flowering of globe artichokes under controlled environment and field conditions. Israel J. Plant Sci. 42: 159-166. Berger, A. 1930. Crassulaceae. In: Engler A and Prantl K (eds) Die naturlichen Pflanzenfamilien, vol 18a. pp. 352-483. Boiteau, P., and L. Allorge-Boiteau. 1995. Kalanchoe (Crassulacées) de Madagascar: Systématique, écophysiologie et phytochimie, Editions Karthala, Paris. Brewbaker, J. L. and B. H. Kwack. 1963. The essential role of calcium ion in pollen germination and pollen tube growth. Amer. J. Bot. 50: 747-858. Bridgen, M. P. 1994. A review of plant embryo culture. HortSicience 29: 1234-1246. Broertjes, C. and L. Leffring. 1972. Mutation breeding of Kalanchoe. Euphytica 21: 415-423. Christopher, J. C., and J. E. Erwin. 2011. Photoperiodic flower induction of several Kalanchoe species and ornamental characteristis of the flowering species. HortScience 46(1): 35-39. Coen, E. S., and E. M. Meyerowitz. 1991. The war of the whorls: genetic interactions controlling flower development. Nature 353(5): 31-37. Currey, C. J., and J. E. Erwin. 2011. Photosynthetic daily light integral impacts growth and flowering of several Kalanchoe species. HortTechnology 21(1): 98-102. De Jeu, M. J., and F. Garriga-Calderé. 1997. Retarded embryo growth and early degeneration of sporophytic tissue are associated with embryo abortion in the interspecific cross Alstroemeria pelegrina × Alstroemeria aurea. Canadian Journal of Botany 75: 916-924. Descoings, B. 1997. Note sur quelques espèces nouvelles de Kalanchoe (Crassulaceae) de Madagascar. J. Bot. Soc. bot. Fr. 4: 79-90. Descoings, B. 2003. Kalanchoe. In: Eggli U, Hattmann HEK (eds) Illustrated handbook of succulent plants. Crassulaceae, Springer Verlag, New York, pp 143-181. Descoings, B. 2006. Kalanchoe rebmannii, Crassulacée nouvelle de Madagascar Par Bernard Descoings. J. Bot. Soc. Bot. France 33: 29-32. Dole, J. M. and H. F. Wilkins. 1999. Kalanchoe. p.403-407. In: Floriculture- Principles and Species. Prentice-Hall, INC. New Jersey, USA. Dubois, A., O. Raymond, M. Maene, S. Baudino, and N. B. Langlade. 2010. Tinkering with the C-Function: A molecular frame for the selection of double flowers in cultivated roses. PLoS ONE 5(2): e9288. Dumas, C. and P. Rogowsky. 2008. Fertilization and early seed formation. Comptes Rendus Biologies 331: 715-725. Eeckhaut. J., J. Van Huylenbroeck, K. Van Laere, and E. Van Bockstaele. 2006. Interspecific hybridization in woody ornamentals: How to deal with barriers ? Acta Hort. 725: 117-126. Faure, N., H. Serieys, E. Cazaux, F. Kaan, and A. Berville. 2002. Partial Hybridization in wide crosses between cultivated sunflower and the perennial Helianthus Species H. mollis and H. orgyalis. Ann. Bot. 89(1): 31-39. Fu, K. T., G. Gilbert and H. Ohba. 1996. Kalanchoe. Flora of China. p.248. Gehrig H. H., H. Rösicke, and M. Kluge. 1997. Detection of DNA polymorphism in the genus Kalanchoe by RAPD-PCR fingerprint and its relationships to infrageneric taxonomic position and ecophysiological photosynthetic behavior of the species. Plant Sci. 125: 41-51. Gehrig, H., O. Gaußmann, H. Marx, D. Schwarzott, and M. Kluge. 2001. Molecular phylogeny of the genus Kalanchoe (Crassulaceae) inferred from nucleotide sequences of the ITS-1 and ITS-2 regions. Plant Sci. 160: 827-835. Gurusamy, V., A. Vandenberg, K. E. Bett. 2007. Manipulation of in vivo pollination techniques to improve the fertilization efficiency of interspecies crosses in the genus Phaseolus. Plant Breeding. 126: 120-124. Harrison, B. J. and L. A. Darby. 1955. Unilateral hybridization. Nature 176(4490): 982. Hartmann, H. T., D. E. Kester, F. T. Davies, and R. L. Geneve. 2002. The development of seeds. p.113-140. Hartmann and Kester’s plant propagation: principles and pratices. Prenntice Hall, Upper Saddle River, N. J. Hisamatsu, T., M. Koshika, N. Oyama, and L. N. Mander. 1999. The relationship between endogenous gibberellins and rosetting in Eustoma grandiflorum. J. Jpn. Soc. Hort. Sci. 68:433-527. Hisamatsu,T., and M. Koshioka. 2000. Cold treatments enhance responsiveness to gibberellins in stock (Matthiola incana (L.) R. Br.). J. Hortic. Sci. Biotechnol. 75:672-678. Horn, W. 2002. Breeding methods and breeding research. p.47-83 In: Vainstein, A. (Eds.), Breeding for Ornamental: Classical and Molecular Approaches. Kluwer Academic Publishers, Netherlands. Huang, C. H., and C. Y. Chu. 2012. The flower development and photoperiodism of native Kalanchoe spp. in Taiwan. Sci. Hort. 146: 59-64. Izumikawa, Y., I. Nakamura, and M. Mii. 2007. Interspecific hybridization between Kalanchoe blossfeldiana and several wild Kalanchoe species with ornamental value. Acta Hort. 743: 59-65. Izumikawa, Y., S. Takei, I. Nakamura, and M. Mii. 2008. Production and characterization of inter-sectional hybrids between Kalanchoe spathulata and K. laxiflora ( = Bryophyllum crenatum). Euphytica. 163: 123-130. Jacobsen, H. 1970. Das Sukkulentenlexikon. Verlag. Jena. Jepsen, K., and E. Christensen. 2008. Double-type Kalanchoe interspecific hybrids. U.S. Patent No. 7,847,150 B2. Jepsen, K., and E. Christensen. 2010. Method for breeding double-type Kalanchoe interspecific hybrids. U.S. Patent No. 7,453,032 B2. Johnson, E. L. 1948. Response of Kalanchoe tubiflora to X-irradiation. Plant Physiol. 23(4): 544-556. Kashihara, Y., K. Shinoda, H. Araki, and Y. Hoshino. 2012. Towards intergeneric hybridization between Alstroemeria L. and Bomarea Mirb. Floriculture and Ornamental Biotechnology. 6(2): 146-149. Kato, A. 1997. Induced single fertilization in maize. Sex. Plant Reprod. 10(2): 96-100. Kikuchi, S., H. Kino, H. Tanaka, and H. Tsujimoto. 2007. Pollen tube growth in cross combinations between Torenia fournieri and fourteen related species. Breed. Sci. 57: 117-122. King, R.W., and L. T. Evans. 2003. Gibberellins and flowering of grasses and cereals: prizing open the lid of the ‘florigen’ black box. Ann. Rev. Plant Biol. 54: 307-328. Kinoshita, T. 2007. Reproductive barrier and genomic imprinting in the development of flowering plants. Genes Genet. Syst. 82: 177-186. Kishi, F., Y. Kagami, M. Shinohara, S. Hatano and H. Tsurushima. 1994. Production of an interspecific hybrid in Gypsophila by ovule-embryo culture. Euphytica 74: 85-90. Kluge, M., and I. P. Ting. 1978. Crassulacean Acid Metabolism: Analysis of an ecological adaptation. Ecological Studies 30. Springer-Verlag, Berlin. Kluge, M., and J. Brulfert. 1996. Crassulacean Acid Metabolism in the Genus Kalanchoe: Ecological, Physiological and Biochemical Aspects. In: K. Winter, J.A.C. Smith (eds.). Crassulacean Acid Metabolism. Biochemistry, Ecophysiology and Evolution, Ecological Studies 114. Springer. Verlag. Berlin. pp.324-335. Kluge, M., J. Brulfert, D. Ravelomanana, J. Lipp, and H. Ziegler. 1991. Crassulacean Acid Metabolism in Kalanchoe species selected at various climatic zones of Madagascar: a survey by d13C analysis, Oecologia 88: 407-414. Kluge, M., J. Brulfert, J. Lipp, D. Ravelomanana, and H. Ziegler. 1993. A comparative study by d13C-analysis of crassulacean acid metabolism (CAM) in Kalanchoe (Crassulaceae) species of Africa and Madagascar. Botanica Acta 106: 320-324. Kluge, M., J. Brulfert, W. Rauh, D. Ravelomanana, and H. Ziegler. 1995. Ecophysiological studies on the vegetation of Madagascar: a δ13C and δD survey for metabolism (CAM) among orchids from montane forests and succulence from the xerophytic thorn-bush, Isotopes Environ. Health Studies 31: 1-20. Kroon, G. H., J. De Jong and L. W. D. Van Raamsdonk. 1989. Evaluation of the genus Kalanchoe for breeding of new cutflowers and potplants. Acta Hort. 252: 111-116. Lewis, D., and L. K. Crowe. 1958. Unilaterial interspecific incompatibility in flowering plants. Heredity 12: 233-256. Li, Y. H., X. Y. Hou, L. Lin, S. X. Jing, and M. Q. Deng. 2000. Abnormal pollen germination and embryo abortion in the interspecific cross, Fragaria × ananassa × F. vesca, as related to cross-incompatibility. J. Jpn. Soc. Hort. Sci. 69: 84-89. Michaels, S. D., and R. M. Amasino. 2000. Memories of winter: vernalization and the competence to flower. Plant Cell Environ. 23(11): 1145-1153. Michniewicz, M. and A. Lang. 1962. Effect of nine different gibberellins on stem elongation and flower formation in cold requiring and photoperiodic plants growth under non-inductive conditions. Planta 58(5): 549-563. Miyajima, D., 2006. Ovules that failed to form seeds in zinnia (Zinnia violacea Cav.). Sci. Hortic. 107(2): 176-182. Morgan, E. R., G. K. Burge, J. F. Seelye, J. E. Grant, and M. E. Hopping. 1995. Interspeccific hybridization between Limonium perigrinnum Bergius and Limonium purperatum L. Euphytica 83: 215-224. Nakornthap, A. 1974. Radiation induce somatic mutation in Kalanchoe (Kalanchoe laciniata). Mut. Breed. Newsl. 3: 14. Nell, T. A. 1993. Prolonging shelf performance-postproduction care and handling. p.57-58. In: Flowering Potted Plants: Ball INC. Illinois, USA. Nimura, M., J. Kato, M. Mii, and K. Morioka. 2003. Unilateral compatibility and genotypic difference in crossability in interspecific hybridization between 121 Dianthus caryophyllus L. and Dianthus japonicus Thunb. Theor. Appl. Genet. 106: 1164-1170. Osmond, C. B. 1978. Crassulacean acid metabolism: A curiosity in context, Ann. Rev. Plant Physiol. 29: 379-414. Penner, J. 1960. Uber den Einflub von gibberellin auf die photoperiodisch bedingten bluhvorgange bei Bryophyllum. Planta 55: 542-572. Pertuit, A. J. 1977. Influence of temperatures during long-night exposures on growth and flowering of ‘Mace’, ‘Thor’ and ‘Telstar’ kalanchoes. HortScience 12: 48-49. Pertuit, A. J. 1992. Kalanchoe. p.430-447. In: Larson, R. A. (ed.), Introduction to Floriculure, II Edition. Academic Press, San Diego, California, USA. Pharis, R. P. and R. W. King. 1985. Gibberelins and reproductive development in seed plants. Ann. Rev. Plant Physiol. 36:517-568. Reed, S. M. 2005. Embryo rescue. p.235-239. In: Plant development and biotechnology. (R. N. Trigiano and D. J. Gray eds.). CRC Press, Boca Raton, FL. Salisbury, R. A. 1805. Bryophyllum calysinum. Parad Lond t.3 Uhl CH (1948) Cytotaxonomic studies in the subfamilies Crassuloideae, Kalanchoideae and Cotyledonoideae of the Crassulaceae. Am. J. Bot. 33: 695-706. Sanchez, A. M., M. Bosch, and M. Bots. 2004. Pistil factors controlling pollination. The Plant Cell 16: 98-106. Saunders, E. R. 1911. Further experiment on the inheritance of doubleness and other characters in stocks. J. Genet. 1: 303-376. Schwaiger, G., and W. Horn. 1988. Somaclonal variations in micropropagated Kalanchoe hybrids. Acta Hort. 226: 695-698. Sehrleider-Moldrickx, R., and W. Horn. 1985. In vitro propagation of Kalanchoe blossfeldiana hybrids II. Callus and suspension cultures. Cartenbauwissenscbaft 50: 9-13. Shaw, J. M. H. 2008. An investigation of the cultivated Kalanchoe daigremontiana group, with a checklist of Kalanchoe cultivars. Hanburyana 3: 17-79. Skene, K. G. M., and A. Lang. 1964. Native gibberellins and flower formation in Bryophyllum daigremontianum. Plant Physiol. 39(suppl.): X X X VII. Stein, O. L. and A. W. Sparrow. 1963. The effect of chronic gamma irradiation on the growth of Kalanchoe cv. Brilliant Star. Radiat. Bot. 3: 207-222. Stein, O. L. and A. W. Sparrow. 1966. The effect of acute irradiation in air, N2 and CO2 on the growth of the shoot apex and internodes of Kalanchoe cv. Brilliant Star. Radiat. Bot. 6: 187-203. Takada, Y., T. Nakanowatari, J. Sato, K. Hatakeyama, T. Kakizaki, A. I. G. Suzuki, H. Shiba, S. Takayama, A. Isogai and M. Watanabe., 2005. Genetic analysis of novel intra-species unilateral incompatibility in Brassica rapa (syn. Campestris) L. Sex Plant Reprod., 17(5): 211-217. Tang, W. H. and T. C. Huang. 1993. Crassulaceae. p.10-34. In: Huang, T. C. (ed.), Flora of Taiwan Vol. Committee of the Flora of Taiwan, Taipei, Taiwan. Ureshino, K., M. Kawai, and I. Miyajima. 2000. Factors of intersectional unilateral cross incompatibility between several evergreen azalea species and Rhododendron japonicum f. flavum. J. Jpn. Soc. Hort. Sci. 69: 261-265. Van Turl, J. M. 1997. Interspecific hybridization of flower bulbs: A review. Acta Hort. 430: 465-476. Van Tuyl, J. M., K. B. Lim, and M. S. Ramanna., 2002. Interspecific hybridization and introgression. p.47-83. In: Vainstein, A. (ed.), Breeding for Ornamental: Classical and Molecular Approaches. Kluwer Academic Publishers, Netherlands. Van Tuyl, J. M., M. P. Van Diёn, M. G. M. Van Creij, T. C. M. Van Kleinwee, J. Franken, and R. J. Bino. 1991. Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci. 74: 115-126. Van Voorst, A. and J. C. Arends., 1982. The origin and chromosome numbers of cultivars of Kalanchoe blossfeldiana Von Pollen: their history and evolution. Euphytica 31(3): 573-584. Wadhi, M. and H. Y. Mohan Ram. 1967. Shortening the juvenile phase for flowering in Kalanchoe pinnata Pers. Planta 73: 28-36. Williams, E. G., and J. L. Rouse. 1988. Disparate style lengths contribute to isolation of species in Rhododendron. Aust. J. Bot. 36:183-191. Williams, E.G., R.B. Knox, and J.L. Rouse. 1982. Pollination sub-systems distinguished by pollen tube arrest after incompatible interspecific crosses in Rhododendron (Ericaceae). J. Cell Sci. 53: 255-277. Wilson, R. N., J. W. Heckman and C. R. Somerville. 1992. Gibberellin is required for flowering in Arabidopsis thaliana under short days. Plant Physiol. 100: 403-408. Winter, K., and J.A.C. Smith. 1996. Crassulacean Acid Metabolism. Biochemistry, Ecophysiology and Evolution, Ecological Studies 114. Springer, Berlin. Zeevaart, J. A. D. 1958. Flower formation as studied by grafting. Meded. Landbouwhogesch. Wageningen 58: 1-88. Zeevaart, J. A. D. 1962. The juvenile phase in Bryophyllum daigremontianum. Planta 58: 543-548. Zeevaart, J. A. D. 1978. Flower formation in the short-day plant Kalanchoe by grafting with a long-day and a short-long-day Echeveria. Planta 140: 289-291. Zeevaart, J. A. D. 1985. Bryophyllum. p.89-100. In: Halevy, A. H. (ed.), Handbook of Flowering, Vol 2. CRC Press, Boca Raton, FL, USA. Zeevaart, J. A. D. and A. Lang. 1962. The relationship between gibberellin and floral stimulus in Bryophyllum daigremontianum. Planta 58: 531-542. http://newplant.afa.gov.tw/。2013年6月。植物品種權公告查詢系統。農糧署全球資訊網。 http://www.calandiva.com/. June, 2013. The history of a diva. Calandiva®. http://www.queen.dk/en-business.aspx. June, 2013. Queen.dk-Knud Jepsen A/S-Business.
摘要: 本試驗使用重瓣長壽花品種:Kalanchoe blossfeldiana ‘Hayworth’、K. blossfeldiana ‘Leonardo’、K. ‘Garambi’s Gold’、K. ‘Peach Fairy’,單瓣長壽花品種:K. blossfeldiana ‘Simone’, K. ‘Pearl’,Kalanchoe節的物種:K. farinacea Balf.f.、K. garambiensis Kudo ‘Purple Leaf’、K. garambiensis Kudo ‘Green Leaf’、K. humilis Britten、K. luciae Raym.-Hamet、 K. marmorata Baker、K. nyikae Engl.,以及Bryophyllum節的物種:K. beauverdii Raym.-Hamet、K. gastonis-bonnieri Raym.-Hamet & H.Perrier、K. pinnata (Lam.) Pers.、K. poincarei Raym.-Hamet & H.Perrier、K. rebmannii Desc.、K. ‘Wendy’等。進行花粉活力檢定、種間雜交試驗,並調查雜交後代植株與開花特性。又Bryophyllum節的物種和Kalanchoe節的K. luciae及K. marmorata在進行前述試驗前先進行GA3處理對開花促進的試驗。 當噴施GA3 25 mg/L以上時,K. luciae、K. marmorata以及K. gastonis-bonnieri之開花率皆達100 %的效果,不噴施GA3者,開花率為0 %。而噴施GA3與否,對於K. rebmannii的開花沒有影響,且到花日數並未減少。 重瓣或單瓣長壽花品種的花粉活力介於2.6 %至16.3 %之間,而物種中花粉發芽率最高的是K. nyikae,大約89.0 %,其次為K. garambiensis ‘Purple Leaf’的88.9 %,最低者為K. humillis,花粉產生滲漏,花粉發芽率為0 %。 Kalachoe節內的物種與長壽花品種之正反交,以K. blossfeldiana ‘Hayworth’作為母本與K. luciae雜交時方可獲得種子,結種率為0.4 %,種子發芽率為11.1 %。而K. blossfeldiana ‘Hayworth’與K. nyikae的雜交組合,無論正交或反交,雖都可結果,但並無種子產生。當重瓣長壽花K. ‘Peach Fairy’為母本與K. nyikae為父本雜交,可獲得0.1 %的結種率,而種子發芽率為13.3 %。反之K. nyikae作為母本時,則無任何種子產生。 Kalanchoe節的長壽花品種與Bryophyllum節的物種正反交後,若K. blossfeldiana ‘Hayworth’作為母本與K. pinnata、K. poincarei、K. rebmannii或K. ‘Wendy’雜交,其結種率分別為0.3、0、1.0或0.4 %,其種子發芽率分別為0.6、0、4.4或0.5 %。反之,Bryophyllum節的物種作為母本時,結種率皆為0。重瓣長壽花K. ‘Peach Fairy’與K. pinnata、K. poincarei或K. ‘Wendy’的雜交中,只有以K. ‘Peach Fairy’作為母本與K. ‘Wendy’雜交,才有獲得雜交後代,結種率為0.1 %,種子發芽率為25.0 %。 種間雜交後代的形態特徵大多介於兩親本中間。而使用盾狀葉的K. nyikae作為親本,子代會在葉片基部出現葉耳。利用K. rebmannii作為親本,則子代多會在植株出現毛茸。Kalanchoe節的物種與葉緣會產生小苗的Bryophyllum節的物種雜交後,後代皆無遺傳到葉緣產生小苗的特性。多數異節雜交的後代,小花朵呈現向上開放,但K. blossfeldiana ‘Hayworth’與K. rebmannii或K.‘Wendy’的雜交後代中有出現花朵完全朝下開放的子代。
In this research, the pollen viability test and interspecific crossing of Kalanchoe cutivars double type: K. blossfeldiana ‘Hayworth’, K. blossfeldiana ‘Leonardo’, K. ‘Garambi’s Gold’, K. ‘Peach Fairy’, and single type: K. blossfeldiana ‘Simone’, K. ‘Pearl’, and species in section Kalanchoe: K. farinacea Balf.f., K. garambiensis Kudo ‘Purple Leaf’, K. garambiensis Kudo ‘Green Leaf’, K. humilis Britten, K. luciae Raym.-Hamet, K. marmorata Baker, K. nyikae Engl., and species in section Bryophyllum: K. beauverdii Raym.-Hamet, K. gastonis-bonnieri Raym.-Hamet & H.Perrier, K. pinnata (Lam.) Pers., K. poincarei Raym.-Hamet & H.Perrier, K. rebmannii Desc. and K. ‘Wendy’ were conducted. And the characteristics of the interspecific offsprings were inspected. Before the previous works, the species of secton Bryophyllum and K. luciae and K. marmorata were sprayed GA3 for forcing flowering. Spaying of GA3 could induce flowering of K. luciae, K. marmorata and K. gastonis-bonnieri, and all flowering rates are 100 %. However, flowering of K. rebmannii didn’t affect by spaying GA3 as well as shorten the days to flower of K. rebmannii. Pollen germination rates in Kalanchoe cutivars ranged from 2.6 % to 16.3 %, and the highest pollen germination in Kalanchoe species was K. nyikae, about 89.0 %, followed by K. garambiensis ‘Purple Leaf’ 88.9 %. The lowest pollen germination was in K. humilis, about 0 %, and the pollen occurred leakage. The reciprocal crossing between section Kalanchoe cultivars and section Kalanchoe species were conducted. When K. blossfeldiana ‘Hayworth’ as maternal donor crossed with K. luciae, the fertility and germination were 0.4 or 11.1 %, respectively. Although it got fruit in the reciprocal crossing between K. blossfeldiana ‘Hayworth’ and K. nyikae, the fertility was at 0 %. Whereas, K. ‘Peach Fairy’ as maternal donor crossed with K. nyikae, the fertilities was at 0.1 % and the germination rate was at 13.3 %. On the contrary, it didn’t get any seeds when K. nyikae as maternal donor. The inter-sectional crossing between section Kalanchoe cultivars and section Bryophyllum species. When K. blossfeldiana ‘Hayworth’ as maternal donor crossed with K. pinnata, K. poincarei, K. rebmannii or K. ‘Wendy’, the fertility was at 0.3, 0, 1.0 or 0.4 %, respectively, and the germination rate was at 0.6, 0, 4.4 or 0.5 %. On the contrary, it didn’t get any seeds when section Bryophyllum species as maternal donors. When K. ‘Peach Fairy’ as maternal donor crossed with K. pinnata, K. poincarei or K. ‘Wendy’, only K. ‘Peach Fairy’ × K. ‘Wendy’ produced offsrings, and the fertility or germination rate was at 0.1 or 25.0 %, respectively. The morphological characters of the inter-sectional hybrids are mostly intermediate between parents. When using K. nyikae as parent, there were auricles on the leave base of hybrids. K. rebmannii produced hybrids with trichomes. The intersectional hybrids couldn’t inherit the epiphyllous bud formation around the leaf margin from section Bryophyllum species. The inter-sectional hybrids changed their angle of flowers, almost of their flowers were upwards. However, there were inter-sectional hybrids with flowers downwards in K. blossfeldiana ‘Hayworth’ crossed with K. rebmannii or K.‘Wendy’.
URI: http://hdl.handle.net/11455/29420
其他識別: U0005-0708201313250500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0708201313250500
顯示於類別:園藝學系

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。