請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/29420
標題: 燈籠草屬同節物種或異節物種之種間雜交
The Interspecific Crossing between Sectional Species or Intersectional Species of Kalanchoe
作者: 盧勝鍵
Lu, Sheng-Jian
關鍵字: 燈籠草屬
interspecific hybridization
出版社: 園藝學系所
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摘要: 本試驗使用重瓣長壽花品種:Kalanchoe blossfeldiana ‘Hayworth’、K. blossfeldiana ‘Leonardo’、K. ‘Garambi’s Gold’、K. ‘Peach Fairy’,單瓣長壽花品種:K. blossfeldiana ‘Simone’, K. ‘Pearl’,Kalanchoe節的物種:K. farinacea Balf.f.、K. garambiensis Kudo ‘Purple Leaf’、K. garambiensis Kudo ‘Green Leaf’、K. humilis Britten、K. luciae Raym.-Hamet、 K. marmorata Baker、K. nyikae Engl.,以及Bryophyllum節的物種:K. beauverdii Raym.-Hamet、K. gastonis-bonnieri Raym.-Hamet & H.Perrier、K. pinnata (Lam.) Pers.、K. poincarei Raym.-Hamet & H.Perrier、K. rebmannii Desc.、K. ‘Wendy’等。進行花粉活力檢定、種間雜交試驗,並調查雜交後代植株與開花特性。又Bryophyllum節的物種和Kalanchoe節的K. luciae及K. marmorata在進行前述試驗前先進行GA3處理對開花促進的試驗。 當噴施GA3 25 mg/L以上時,K. luciae、K. marmorata以及K. gastonis-bonnieri之開花率皆達100 %的效果,不噴施GA3者,開花率為0 %。而噴施GA3與否,對於K. rebmannii的開花沒有影響,且到花日數並未減少。 重瓣或單瓣長壽花品種的花粉活力介於2.6 %至16.3 %之間,而物種中花粉發芽率最高的是K. nyikae,大約89.0 %,其次為K. garambiensis ‘Purple Leaf’的88.9 %,最低者為K. humillis,花粉產生滲漏,花粉發芽率為0 %。 Kalachoe節內的物種與長壽花品種之正反交,以K. blossfeldiana ‘Hayworth’作為母本與K. luciae雜交時方可獲得種子,結種率為0.4 %,種子發芽率為11.1 %。而K. blossfeldiana ‘Hayworth’與K. nyikae的雜交組合,無論正交或反交,雖都可結果,但並無種子產生。當重瓣長壽花K. ‘Peach Fairy’為母本與K. nyikae為父本雜交,可獲得0.1 %的結種率,而種子發芽率為13.3 %。反之K. nyikae作為母本時,則無任何種子產生。 Kalanchoe節的長壽花品種與Bryophyllum節的物種正反交後,若K. blossfeldiana ‘Hayworth’作為母本與K. pinnata、K. poincarei、K. rebmannii或K. ‘Wendy’雜交,其結種率分別為0.3、0、1.0或0.4 %,其種子發芽率分別為0.6、0、4.4或0.5 %。反之,Bryophyllum節的物種作為母本時,結種率皆為0。重瓣長壽花K. ‘Peach Fairy’與K. pinnata、K. poincarei或K. ‘Wendy’的雜交中,只有以K. ‘Peach Fairy’作為母本與K. ‘Wendy’雜交,才有獲得雜交後代,結種率為0.1 %,種子發芽率為25.0 %。 種間雜交後代的形態特徵大多介於兩親本中間。而使用盾狀葉的K. nyikae作為親本,子代會在葉片基部出現葉耳。利用K. rebmannii作為親本,則子代多會在植株出現毛茸。Kalanchoe節的物種與葉緣會產生小苗的Bryophyllum節的物種雜交後,後代皆無遺傳到葉緣產生小苗的特性。多數異節雜交的後代,小花朵呈現向上開放,但K. blossfeldiana ‘Hayworth’與K. rebmannii或K.‘Wendy’的雜交後代中有出現花朵完全朝下開放的子代。
In this research, the pollen viability test and interspecific crossing of Kalanchoe cutivars double type: K. blossfeldiana ‘Hayworth’, K. blossfeldiana ‘Leonardo’, K. ‘Garambi’s Gold’, K. ‘Peach Fairy’, and single type: K. blossfeldiana ‘Simone’, K. ‘Pearl’, and species in section Kalanchoe: K. farinacea Balf.f., K. garambiensis Kudo ‘Purple Leaf’, K. garambiensis Kudo ‘Green Leaf’, K. humilis Britten, K. luciae Raym.-Hamet, K. marmorata Baker, K. nyikae Engl., and species in section Bryophyllum: K. beauverdii Raym.-Hamet, K. gastonis-bonnieri Raym.-Hamet & H.Perrier, K. pinnata (Lam.) Pers., K. poincarei Raym.-Hamet & H.Perrier, K. rebmannii Desc. and K. ‘Wendy’ were conducted. And the characteristics of the interspecific offsprings were inspected. Before the previous works, the species of secton Bryophyllum and K. luciae and K. marmorata were sprayed GA3 for forcing flowering. Spaying of GA3 could induce flowering of K. luciae, K. marmorata and K. gastonis-bonnieri, and all flowering rates are 100 %. However, flowering of K. rebmannii didn’t affect by spaying GA3 as well as shorten the days to flower of K. rebmannii. Pollen germination rates in Kalanchoe cutivars ranged from 2.6 % to 16.3 %, and the highest pollen germination in Kalanchoe species was K. nyikae, about 89.0 %, followed by K. garambiensis ‘Purple Leaf’ 88.9 %. The lowest pollen germination was in K. humilis, about 0 %, and the pollen occurred leakage. The reciprocal crossing between section Kalanchoe cultivars and section Kalanchoe species were conducted. When K. blossfeldiana ‘Hayworth’ as maternal donor crossed with K. luciae, the fertility and germination were 0.4 or 11.1 %, respectively. Although it got fruit in the reciprocal crossing between K. blossfeldiana ‘Hayworth’ and K. nyikae, the fertility was at 0 %. Whereas, K. ‘Peach Fairy’ as maternal donor crossed with K. nyikae, the fertilities was at 0.1 % and the germination rate was at 13.3 %. On the contrary, it didn’t get any seeds when K. nyikae as maternal donor. The inter-sectional crossing between section Kalanchoe cultivars and section Bryophyllum species. When K. blossfeldiana ‘Hayworth’ as maternal donor crossed with K. pinnata, K. poincarei, K. rebmannii or K. ‘Wendy’, the fertility was at 0.3, 0, 1.0 or 0.4 %, respectively, and the germination rate was at 0.6, 0, 4.4 or 0.5 %. On the contrary, it didn’t get any seeds when section Bryophyllum species as maternal donors. When K. ‘Peach Fairy’ as maternal donor crossed with K. pinnata, K. poincarei or K. ‘Wendy’, only K. ‘Peach Fairy’ × K. ‘Wendy’ produced offsrings, and the fertility or germination rate was at 0.1 or 25.0 %, respectively. The morphological characters of the inter-sectional hybrids are mostly intermediate between parents. When using K. nyikae as parent, there were auricles on the leave base of hybrids. K. rebmannii produced hybrids with trichomes. The intersectional hybrids couldn’t inherit the epiphyllous bud formation around the leaf margin from section Bryophyllum species. The inter-sectional hybrids changed their angle of flowers, almost of their flowers were upwards. However, there were inter-sectional hybrids with flowers downwards in K. blossfeldiana ‘Hayworth’ crossed with K. rebmannii or K.‘Wendy’.
URI: http://hdl.handle.net/11455/29420
其他識別: U0005-0708201313250500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0708201313250500


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