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Analysis of Quality Criteria and Mineral Nutrient of in vitro Phalaenopsis Plantlets
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Phalaenopsis orchid is one of the most important economic pot plants in Taiwan. The present study was initiated to investigate and understand the condition of in vitro Phalaenopsis plantlets in Taiwan industry that hope could be used to improve the quality of Phalaenopsis plantlets in the future. At first, we found that in vitro plantlets with 3 to 4 leaves, more than 2 roots and with shoot root dry weight ratio about 1 to 2 have a better growth condition when cultivated in greenhouse. Higher shoot/root dry weight ratio resulted in the poor growth of plantlets after cultivation. Regardless of medium composition, Phalaenopsis have a high absorption of nitrogen (N) and potassium (K). Besides, experiments also found that Phalaenopsis also have a high absorption of manganese (Mn). In the different plant density and culture vessel experiments, lower plant density didn’t result in the higher leaf and root number. Lower plant density could result in the bigger plant size, but we assume that medium have a bigger effect than plant density. The number of leaf from the plantlets that were culture in plastic container previously was decreased 1 or 2 after cultivated in the greenhouse. Different plant density and vessel type didn’t affect the mineral absorption and carbohydrates content in the plantlets. In the study of mineral nutrient and carbohydrates content of in vitro medium and leaf tissue from different growth stage, with additional of coconut water in the multiplication medium, it resulted in the increment of K concentration. While, additional of banana homogenate resulted in the higher phosphorus (P) concentration. pH value of the medium from 5.4 was decline to 4.5 to 4.8 after autoclaved. In the rooting stage, it’s found that the concentration of macro-element was lower than multiplication stage. We suggest that lower content of macro-element in rooting growth stage is one factor that caused the presence leaf yellowing and black spotting. The total soluble sugar (TSS) concentration was stable during the multiplication to rooting stage 1 and increased during rooting stage 2. There was no significance different in the starch concentration during in vitro culture. The TSS and starch concentration decreased after 4 months of cultured in greenhouse. This study was showed some phenomenon in the growth and development of in vitro phalaenopsis plantlets. We suggest that through this study, we could improve the quality of in vitro phalaenopsis plantlets in the future.
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