Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3034
標題: 不同生化反應器及富氧操作對獸疫鏈球菌醱酵生產透明質酸之探討
Study of various bioreactors and oxygen-rich operation on hyaluronic acid production by Streptococcus zooepidemicus fermentation
作者: 陳美錚
Chen, Mei-Jhong
關鍵字: 獸疫鏈球菌
Streptococcus zooepidemicus
透明質酸
富氧操作
分子量
生化反應器
hyaluronic acid
oxygen-rich operation
molecular weight distribution
net draft tube bioreactor
出版社: 化學工程學系所
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摘要: 本研究以Streptococcus zooepidemicus ATCC 39920醱酵探討不同反應器及富氧操作對生產透明質酸(Hyaluronic Acid, HA)之影響,進而提昇透明質酸產量,並比較其分子量的變化。研究中發現,使用網管氣舉式醱酵槽培養,通氣量3 vvm,pH 7.0,溫度37 ℃時,醱酵時間在4 - 6小時,其透明質酸的產速較快。因此,在醱酵時間第六小時進行富氧操作,在通入氧氣為30%時,其透明質酸產量可從原來的1.72 g / L增加到3.05 g / L,提升透明質酸產量約為1.7倍,菌體量則無明顯變化;若在第六小時通入40%氧氣時,透明質酸產量則維持在第六小時的產量1.55 g/L,不再往上提升。研究顯示攪拌式醱酵槽,轉速為200 rpm,通氣量3 vvm,pH 7.0,溫度為37 ℃,培養時間12小時,其平均分子量為1.0×103 kDa;比較使用氣舉式醱酵槽生產透明質酸,其分子量的範圍則是1.9×102 kDa,以上結果顯示,使用氣舉式醱酵槽能生產較低分子量之透明質酸。
In this study, different bioreactors were applied to the production of hyaluronic acid (HA) by Streptococcus zooepidemicus ATCC 39920. The HA yield and its molecular weight were also compared. The results indicated the fast HA production rate was observed at 4-6 h when operated at 3vvm, pH 7.0 and 37 ℃ on the net draft tube bioreactor. When applying the oxygen-rich operation (30% oxygen-rich at 6h), the HA yield was increased from 1.72 to 3.05 g /L, a 1.7-fold increase as compared to that without oxygen-rich. When using stirred tank bioreactor, the high molecular weight HA (1.0×103 kDa) was produced. In contrast, the low molecular weight HA (1.9×102 kDa) could be obtained when using net draft tube bioreactor.
URI: http://hdl.handle.net/11455/3034
其他識別: U0005-2208201215273700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2208201215273700
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