Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30646
標題: 楊桃花姬捲葉蛾之生殖行為及其性費洛蒙組成份之鑑定與應用
Reproductive Behavior of the Carambola Fruit Borer, Eucosma notanthes Meyrick, and Identification and Application of its Sex Pheromone Components
作者: 洪巧珍
Hung, Chau Chin
關鍵字: carambola
楊桃
carambola fruit borere
Eucosma notanthes
sex pheromone
reproductive behavior
bioassay
application
Z-8-dodecenyl acetate
Z-8-dodecenol
花姬捲葉蛾
性費洛蒙
生殖行為
生物檢定
田間應用
順 -八-十二烯醇乙酸酯
順 -八-十二烯醇
出版社: 昆蟲學系
摘要: 楊桃花姬捲葉蛾(Eucoasma notanthes Meyrick )之雌、雄蛹在25±2℃、70±5% RH及 L:D=12:12條件下,於見光後開始羽化,以見光後5-8小時達羽化高峰。雌蛾表現發情及交尾行為的時刻皆在見光後2小時達高峰,交尾高峰期發生於1-4日齡成蛾。雌蛾產卵時刻於黑暗後1小時達高峰。利用氣相色層分析儀及氣相色層--質譜儀鑑定楊桃花姬捲葉蛾之性費洛蒙萃取液之組成份,結果顯示順-8-十二烯醇乙酸酯(Z-8-dodecenyl acetate, Z8-12:Ac)及順-8-十二烯醇(Z-8-dodecenol, Z8-12:OH)為其性費洛蒙組成份,兩者比例約為1.0:2.7,平均每隻雌蟲萃取量分別為1.20 及3.21 ng。田間誘蟲結果以 Z8-12:Ac/Z8-12:OH=1:1 時,效果最佳。以兩種室內生物檢定方法:風洞及六角型轉盤試驗與田間試驗比較,來檢定不同性費洛蒙配方對花姬捲葉蛾雄蟲之活性。在檢定雄蛾對性費洛蒙反應時段、不同雙鍵位置之十二烯醇乙酸酯、Z8-12:Ac不同劑量及Z8-12:Ac與E8-12:Ac不同混合比例時,風洞、轉盤及田間試驗結果一致,以Z8-12:Ac純度較高之配方(>99%)之誘蟲效果最佳,惟轉盤及田間試驗均以1 mg之誘引效果最佳,風洞則以10 μg以下才能表現誘蟲效果。檢定Z8-12:Ac/Z8-12:OH不同混合比例對雄蛾之誘引性,田間試驗結果顯示以1 mg,Z8-12:Ac/Z8-12:OH=1/1的配方最具誘蟲效果,風洞及轉盤試驗結果以100/0及100/5的配方誘蟲效果較佳。楊桃園每公頃懸掛40~80個性費洛蒙誘蟲器,長期大量誘殺花姬捲葉蛾,可有效降低其密度達1.0-4.5 insects/trap/ week,與非誘殺區比較,密度降低率達75.5~89.4%。楊桃果園每公頃施放53.28 g之Z8-12:Ac性費洛蒙干擾劑進行交尾干擾試驗防治花姬捲葉蛾,於5個月處理期間,其誘引抑制率達100%,田間及網箱中之交尾抑制率分別達97及89%,果實被害率減少約71%。
Behavior of eclosion, mating and oviposition of the carambola fruit borer (CFB), Eucosma notanthes Meyrick, were observed under the condition of 25±2°C, 70±5% RH, and L:D=12:12 photoperiod. Eclosion reached peak from 5 to 8 hr after light on, while the peaks of calling and mating activity occurred at 2 hr after light on. However, oviposition took place after light off, and reached a peak in 1 hr after light off. Pairing with different ages of adult moths for 24 hr, the peak of mating activity was found in 1-4 days after eclosion. The compounds, Z-8-dodecenyl acetate (Z8-12:Ac) and Z-8-dodecenol (Z8-12:OH), were isolated and identified as major components from CFB pheromone glands by gas chromato-graphy and mass spectra of gland components. The blend ratio of Z8-12:Ac/Z8-12:OH was 1.0/2.7 with the amount 1.20 and 3.21 ng per female extracted, respectively. Based on the field, turntable and wind tunnel test results, Z8-12:Ac was the main component. The compound, Z8-12:Ac, mixed with Z8-12:OH by equal amount obtained the most attractiveness to CFB. The attractiveness of different fomulation lures to CFB using two bioassay methods, turntable and wind tunnel, were studied in laboratory and their results were compared with field test. The attractive period of sex lures to males was 2 to 4 hr after light on, and the peak for attracting was 3 hr after light on in field. The attractive period was found 2-5 hr after light on by wind tunnel test. The results of attractiveness of Z7-12:Ac, Z8-12:Ac, and Z9-12:Ac to males were same as field tests. Only lures of Z8-12:Ac showed bioactivity to males. The results of attractiveness of different dosages of Z8-12:Ac to males in turntable tests were similar to field tests. Dosage at 1 mg obtained the best attractiveness compared with others by turntable and field tests, while lures below 10 μg exhibited attraction to males in wind tunnel. The attractiveness of different blend ratios of Z8-12:Ac/E8-12:Ac to males was the same as that of turntable (1 mg), wind tunnel (10 μg), and field tests (1 mg). The blend ratios of Z8-12:Ac/E8-12:Ac were 100/0 or 99.5/0.5 exhibited more attractive to males than others. The attractive- ness of different blend ratios of Z8-12:Ac/Z8-12:OH was different by turntable, wind tunnel, and field test methods. The best results of blend ratios with field test, turntable, and wind tunnel were Z8-12:Ac/Z8-12:OH =100/100, 100/0, and 100/5, respectively. Density of CFB was declined to 1.0-4.5 insects/trap/week when the orchards were treated with 40-80 traps/ha to mass trap CFB. Percent of density reduction compared to un-treated orchards was 75.4-89.4%. In order to control CFB by mating disruption, carambola orchards were treated with 53.28 g/ha of Z8-12:Ac disruptants during at 5 month period of test. Percent of attraction inhibition, mating inhibition in cage and field of CFB, and damaged fruits reduction compared with insecticides treated orchards were 100%, 89.4 and 97.45, and 70.9%, respectively.
URI: http://hdl.handle.net/11455/30646
Appears in Collections:昆蟲學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.