Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30693
標題: 進口米害蟲之監測及快速鑑定技術
Monitoring and Rapid Identification Techniques for Stored Product Insects in Imported Rice
作者: 姚美吉
Yao, Me-Chi
關鍵字: imported rice
進口米
stored-product insects
quarantine
light trap
SCAR
multiplex PCR
積穀昆蟲
檢疫
燈光誘引器
序列特徵化增幅區域
複合式PCR
出版社: 昆蟲學系所
引用: Agustí N, de Vicente MC, Gabarra R. 1999. Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction-based technique for predator gut analysis. Mol Ecol 8: 1467-1474. Agustí N, de Vicente MC, Gabarra R. 2000. Developing SCAR markers to study predation on Trialeurodes vaporariorum. Insect Mol Biol 9: 263-268. Athanassiou CG, Kavallieratos NG, Trematerra P. 2006. Responses of Sitophilus oryzae (Coleoptera: Curculionidae) and Tribolium confusum (Coleoptera: Tenebrionidae) to traps baited with pheromones and food volatiles. European J Entomol 103: 371-378. Ball SL, Hebert PDN, Burian SK, Webb JM. 2005. Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. J North Am Benthol Soc 24: 508-524. Bashir TL, Birkinshaw A, Farman D, Hall DR, Hodges RJ. 2003. Pheromone release by Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) in the laboratory: daily rhythm, inter-male variation and association with body weight and/or boring activity. J Stored Prod Res 39: 159-169. Black IV WC. 1993. PCR with arbitrary primers: approach with care. Insect Mol Biol 2: 1-6. Blainey AD, Topping MD, Ollier S, Davies RJ. 1989. Allergic respiratory disease in grain workers: the role of storage mites. J Allergy Clin Immunol 84: 296-303. Birch LC. 1953. Experimental background to the study of the distribution and abundance of insects. I. The influence of temperature, moisture and food on the innate capacity for increase of three grain beetles. Ecology 34: 698-711. Chang SC, Yao MC, Chen YH, Lu KH. 2011. Current status and future prospects of molecular biological techniques used in the species identification of agricultural pests. In: Symposium on Diagnosis and Identification of Crop Pests in Taiwan. 2011 Sep 8-9; Taipei: Research Center for Plant Medicine. pp 145-163. Chang TC, Liu TY, Leu LK. 1981. Comparison of quality and insect occurrence for the paddy rice stored in bag and in bulk. Natl Sci Counc Monthly 9: 592-602. (in Chinese) Chen W, Hoy JW, Schneider RW. 1992. Species-specific polymorphisms in transcribed ribosomal DNA of five Pythium species. Exp Mycol 16: 22-34. Chiu YC, Wu WJ, Shiao SF, Shih CJ. 2000. The application of RAPD-PCR to develop rapid diagnostic technique for identification of 6 species of Liriomyza spp. (Diptera: Agromyzidae). Chinese J Entomol 20: 293-309. COA. 2010. Taiwan food statistics book 2009. Council of Agriculture, Taiwan ROC. Dhaliwal GS. 1974. Metabolism of fumigants. Bull Grain Technol 12: 132-138. Dowdy AK, McGaughey W. 1996. Using random amplified polymorphic DNA to differentiate strains of the Indian meal moth (Lepidoptera: Pyralidae). Environ Entomol 25: 396-400. Fadamiro HY, Baker TC. 2002. Pheromone puffs suppress mating by Plodia interpunctella and Sitotroga cerealella in an infested corn store. Entomol Exp Appl 102: 239-251. Fletcher LW, Long JS. 1973. Evaluation of an electric grid light trap as a means of sampling populations of the cigarette beetle. Tobacco Sci 17: 37-39. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3: 294-299. Franzolin MR, Gambale W, Cuero RG., Correa B. 1999. Interaction between toxigenic Aspergillus flavus Link and mites (Tyrophagus putrescentiae Schrank) on maize grains: effects on fungal growth and aflatoxin production. J Stored Prod Res 35: 215-224. Gasparich GE, Sheppard WS, Han HY, McPheron BA, Steck GJ. 1995. Analysis of mitochondrial DNA and development of PCR-based diagnostic molecular markers for Mediterranean fruit fly (Ceratitis capitata) populations. Insect Mol Biol 4: 61-67. Gleeson D, Holder P, Newcomb R, Howitt R, Dugdale J. 2000. Molecular phylogenetics of leafrollers: application to DNA diagnostics. N Z Plant Prot 53: 157-162. Green WF, Woolcock AJ. 1978. Tyrophagus putrescentiae: An allergenically important mite. J Clin Allergy 8: 135-144. Greenstone MH, Vandenberg NJ, Hu JH. 2011. Barcode haplotype variation in north American agroecosystem lady beetles (Coleoptera: Coccinellidae). Mol Ecol Resour 11: 629-637. Hebert PDN, Cywinska A, Ball SL, DeWaard JR. 2003. Biological identifications through DNA barcodes. Proc Roy Soc Lond Ser B Biol Sci 270: 313-321. Hidayat P, Phillips TW, Ffrench-Constant RH. 1996. Molecular and morphological characters discriminate Sitophilus oryzae and S. zeamais (Coleoptera: Curculionidae) and confirm reproductive isolation. Ann Entomol Soc Am 89: 645-652. Hodges RJ, Hall DR, Mbugua JN, Likhayo PW. 1998. The responses of Prostephanus truncatus (Coleoptera: Bostrichidae) and Sitophilus zeamais (Coleoptera: Curculionidae) to pheromone and synthetic maize volatiles as lures in crevice and flight traps. Bull Entomol Res 88: 131-139. Howe RW. 1965. A summary of estimates of optimal and minimal conditions for population increase of some stored products insects. J Stored Prod Res 1: 177-184. Hsieh FK, Kao SS. 1978. Ecology of storage insects. pp 83-111. In: Chiu RJ (ed). Diseases and Insect Pests of Rice: Ecology and Epidemiology. Joint Commission on Rural Reconstruction Pub. Taipei. (in Chinese) Hsieh FK, Hung LM, Kao SS, Hus SL. 1980. Estimates of losses of stored rice caused by insects. Plant Prot Bull 22: 385-395. (in Chinese) Hung CC, Hwang JS. 1992. Insect pests in rough rice, brown rice and chaff stored in different kinds of bin in Taiwan. Chinese J Entomol 12: 269-276. (in Chinese) Hung CC, Peng WK. 1985. The attractancy of rice kernels with different degrees of damage on husk to rice and maize weevil. Chinese J Entomol 5: 31-36. (in Chinese) Hung CC, Hsieh FK, Hwang JS. 1990. Stored grain insects monitoring and their chemical control. Chinese J Entomol 10: 169-179. (in Chinese) Hwang JS, Hsieh FK, Kung KS. 1983. Influence of temperature and relative humidity on the development and reproduction of the maize weevil, Sitophilus zeamais Motschulsky. Plant Prot Bull 24:41-52. (in Chinese) Jacob TA. 1996. The effect of constant temperature and humidity on the development, longevity and productivity of Ahasverus advena (Waltl.) (Coleoptera: Silvanidae). J Stored Prod Res 32: 115-121. Kao SS, Tzeng CC. 1992. A survey of the susceptibility of rice moth (Corcyra cephalonica) and angoumois moth (Sitotroga cerealella) to malathion and phoxim. Chinese J Entomol 12: 239-245. (in Chinese) Kethidi DR, Roden DB, Ladd TR, Krell PJ, Retnakaran A, Feng Q. 2003. Development of SCAR markers of the DNA-based detection of the Asian long-horned beetle, Anoplophora glabripennis (Motschulsky). Arch Insect Biochem Physiol 52: 193-204. Kheradmand K, Kamali K, Fathipour Y, Goltapeh EM. 2007. Development, life table and thermal requirement of Tyrophagus putrescentiae (Astigmata: Acaridae) on mushrooms. J Stored Prod Res 43: 276-281. Kjer KM. 2004. Aligned 18S and insect phylogeny. Syst Biol 53: 506-514. Kline DL, Patnaude M, Barnard DR. 2006. Efficacy of four trap types for detecting and monitoring Culex spp. in north central Florida. J Med Entomol 43: 1121-1128. Knulle W, Spadafora RR. 1969. Water vapor sorption and humidity relationships in Liposcelis (Insecta: Psocoptera). J Stored Prod Res 5: 49-55. Laroche A, DeClerck-Floate RA, LeSage L, Floate KD, Demeke T. 1996. Are Altica carduorum and Altica cirsicola (Coleoptera: Chrysomelidae) different species? Implications for the release of A. cirsicola for the biocontrol of Canada thistle in Canada. Biol Control 6: 306-314. Li ZH, Kučerová Z, Zhao S, Stejskal V, Opit G, Qin M. 2011. Morphological and molecular identification of three geographical populations of the storage pest Liposcelis bostrychophila (Psocoptera). J Stored Prod Res 47: 168-172. Liang CJ, Chen TN, Lin C. 1954. Current status of rice storages and damage by stored product insects in Taiwan. Sci Agric 2: 34-41. (in Chinese) Likhayo PW, Hodges RJ. 2000. Field monitoring Sitophilus zeamais and Sitophilus oryzae (Coleoptera: Curculionidae) using refuge and flight traps baited with synthetic pheromone and cracked wheat. J Stored Prod Res 36: 341-353. Lin CK. 1971. Pest of stored grain and grain products in Taiwan (I): studies on the biology of the khapra beetle, Trogoderma granarium Everts. (Coleoptera: Dermestidae). Plant Prot. Bull. 13: 18-24. (in Chinese) Lin CK. 1972. An identification of three species of Lepidopterous larvae attacking stored grain and grain products in Taiwan. Plant Prot Bull 14: 36-40. (in Chinese) Lin CK. 1973. Biological studies on the khapra beetle Trogoderma granarium Everts (Coleoptera: Dermestidae) II. Studies on the effect of temperature in relation to its larval stadium. Plant Prot Bull 15: 86-92. (in Chinese) Lin GW, Lu SL, Huang TY, Shih CL, Wu WJ, Chang CC. 2008. Molecular identification of weevils significant for customs inspection and quarantine importance. Formosan Entomol 28: 43-55 (in Chinese). Lin T. 1968. A survey on the granary insects and their natural enemies in Taiwan. J Agric Res China 17: 39-45. (in Chinese) Lin T, Li CH. 1983. Studies on ecology of khapra beetle, Trogoderma granarium E. J Agric Res China 32: 383-389. (in Chinese) Lin T, Li CH. 1984. The source of insects occurrence of stored rice grain. J Agri Res China 33: 431-436. Liu YC, Chang SC, Chen WH, Shu WB. 2006. The application of single-step nested multiplex polymerase chain reaction for the identification of Rhizoglyphus echinopus, R. robini and R. setosus simultaneously. Plant Prot Bull 48: 101-116. (in Chinese) Lobl I, Smetana A. 2007. Catalogue of palaearctic Coleoptera volume 4. Stenstrup Apollo Books. 935 pp. Lu KH, Chang CM, Chen CY, Chen YJ. 2002. Development of species-specific PCR primers for identification of three key tephritid pests in Taiwan. Plant Prot Bull 44: 255-265. (in Chinese) Lu KH, Chang SC, Hsu JY, Yang JT. 2003. A combination of traditional and modern techniques for insect identification- using the codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), as an example. Plant Prot Bull 45: 359-364. (in Chinese) Manguin S, Kengne P, Sonnier L, Harbach RE, Baimai V, Trung HD, Coosemans M. 2002. SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia. Med Vet Entomol 16: 46-54. Müller GC, Revay EE, Beier JC. 2011. Simplified and improved monitoring traps for sampling sand flies. J Vector Ecol 36: 454-457. Mullis K, Faloona F. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155: 335-350. Nowaczyk K, Obrepalska-Steplowska A, Gawlak M, Throne JE, Olejarski P, Nawrot J. 2009. Molecular techniques for detection of Tribolium confusum infestations in stored products. J Econ Entomol 102: 1691-1695. Odegaard F. 1999. Invasive beetle species (Coleoptera) associated with compost heaps in the Nordic countries. Norw J Entomol 46: 67-78. Okuni T. 1924. On the grain-pest in Formosa part I. In: Formosa, Japan, Department of Agriculture Government Research Institute, Report 9:1-166. (in Japanese) Orui Y, Mizukubo T. 1999. Discrimination of seven Pratylenchus species (Nematoda: Pratylenchidae) in Japan by PCR-RFLP analysis. Appl Entomol Zool 34: 205-211. Papadopoulou SC, Buchelos CT. 2002. Comparison of trapping efficacy for Lasioderma serricorne (F.) adults with electric, pheromone, food attractant and control-adhesive traps. J Stored Prod Res 38: 375-383. Paran I, Michelmore RW. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985-993. Park BS, Lee BH, Kima TW, Renc YL, Lee SE. 2008. Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine. Environ Toxicol Pharmacol 25: 121-126. Peng WK. 1979. The occurrence of coleopterous insect pests in stored japonica, indica and long grain indica type rice. Natl Sci Counc Monthly 7: 602-608. (in Chinese) Peng WK. 1984. Population changes of stored-product insects in warehouses of bagged rice and the effect of phoxim treatment on the population suppression. Phytopathol Entomol NTU 11: 105-114. Peng WK, Yang CC, Lee TK. 1999. Horizontal diffusion of phosphine through grain. Chinese J Entomol 19: 201-207. (in Chinese) Peng WK, Lin HC, Chen CN, Wang CH. 2003. DNA identification of two laboratory colonies of the weevils, Sitophilus oryzae (L.) and S. zeamais Motschulsky (Coleoptera: Curculionidae) in Taiwan. J Stored Prod Res 39: 225-235. Rees D. 2004. Insects of stored products. Collingwood: CSIRO Publ. 181 pp. Russell RC. 2004. The relative attractiveness of carbon dioxide and octenol in CDC- and EVS-type light traps for sampling the mosquitoes Aedes aegypti (L.), Aedes polynesiensis Marks, and Culex quinquefasciatus Say in Moorea, French Polynesia. J Vector Ecol 29: 309-314. Ryoo MI, Cho HW. 1992. Feeding and oviposition preferences and demography of rice weevil (Coleoptera: Curculionidae) reared on mixtures of brown, polished, and rough rice. Environ Entomol 21: 549-555. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. 1985. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350-1354. Sayaboc PD, Gibe AJG, Caliboso FM. 1998. Resistance of Rhizopertha dominica (F.) (Coleoptera: Bostrychidae) to phosphine in the Philippines. Philipp Entomol 12: 91-95. Scheffer SJ, Lewis ML, Joshi RC. 2006. DNA barcoding applied to invasive leafminers (Diptera : Agromyzidae) in the Philippines. Ann Entomol Soc Am 99: 204-210. Simons C, Frati F, Beckenbach A, Crespi B, Liu H, Floors P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87: 651-701. Sudia WD, Chamberlain RW. 1962. Battery operated light trap, an improved model. Mosq News 22: 126-129. Trematerra P, Girgenti P. 1989. Influence of pheromone and food attractants on trapping of Sitophilus oryzae (L.) (Col., Curculionidae): a new trap. J Appl Entomol 108: 12-20. Tzeng CC, Peng WK, Kao SS. 2006. Survey of insect populations in stored rice with blacklight-blue fluorescent light trap. Plant Prot Bull 48: 297-309. (in Chinese) Weller SJ, Friedlander TP, Martin JA, Pashley DP. 1992. Phylogenetic studies of ribosomal RNA variation in higher moths and butterflies (Lepidoptera: Ditrysia). Mol Phylogenet Evol 1: 312-337. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic-markers. Nucleic Acids Res 18: 6531-6535. Wu M, Sun LV, Vamathevan J, Riegler M, Deboy R, Brownlie JC, McGraw EA, Martin W, Esser C, Ahmadinejad N, Wiegand C, Madupu R, Beanan MJ, Brinkac LM, Daugherty SC, Durkin AS, Kolonay JF, Nelson WC, Mohamoud Y, Lee P, Berry K, Young MB, Utterback T, Weidman J, Nierman WC, Paulsen IT, Nelson KE, Tettelin H, O''Neill SL, Eisen JA. 2004. Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: A streamlined genome overrun by mobile genetic elements. PLoS Biol 2: e69. Wu WH. 1999. A survey of stored-product insects on Kaohsiung Harbor in Taiwan. Magazine World Agrochem 185: 86-89. (in Chinese) Yao MC, Lo KC. 1992. Insect species and population densities in stored japonica rice in Taiwan. Chinese J Entomol 12:161-169. (in Chinese) Yao MC, Lee CY, Lu KH. 2009a. Survey and monitoring of insect and mite pests in imported rice. J Taiwan Agric Res 58: 17-30. (in Chinese) Yao MC, Lo KC, Wan YN. 2003. Effects of the milling process and grocery environments on the occurrence of stored-product insects. Plant Prot Bull 45: 101-116. (in Chinese) Yao MC, Lee CY, Yang EC, Lu KH. 2009b. Varieties and population fluctuations of stored-product insects in various rice-storage facilities monitored with light traps. Formosan Entomol 29: 225-237. (in Chinese) Yao MC, Lu KH, Wang YT, Lee CY. 2007. Population fluctuations of insect pests of garlic bulbs (Allium sativum L.) in storehouses monitored with light traps and yellow sticky cards. Plant Prot Bull 49: 171-185. (in Chinese) Zou J, Yang Q, Chen Q, Liu Y, Dong W, Chen S. 2000. Molecular characterization of RAPD and SCAR marker linked to frog-eye leaf spot resistance gene in soybean. Chinese Sci Bull 45: 460-466.
摘要: 台灣自2002年加入世界貿易組織(World Trade Organization, WTO),開始進口稻米長期貯藏,害蟲監測也因此成為後續管理之重要一環。本研究主要在探討進口米貯藏期間之害蟲發生情形,並建立害蟲族群變化之監測方法與分子快速鑑定技術。首先,自2003至2006年間,針對進口米害蟲進行危害調查及進倉後長期監測之結果顯示,進口米檢疫採樣之樣品,含活蟲之比例為12%,但並未發現檢疫害蟲;後續貯藏階段以袋內取樣監測之全部樣品,共檢測出11種害蟲及1種害蟎。在常溫倉中,害蟲於貯藏三個月後發生,平均密度每公斤低於10隻;六個月後,害蟲則普遍大量發生,密度每公斤高於35隻;低溫倉(15-18°C)貯藏,害蟲延至貯藏六個月後才開始發生,且自2004年之後害蟲發生密度每公斤低於1隻,遠較常溫倉為低。 其次,利用部分積穀害蟲善飛及具趨光性,評估應用燈光誘引器監測害蟲族群變化之結果顯示,在3處(林內、莿桐、六甲)常溫倉捕獲之初級及次級害蟲種類相極為相似,其中對初級害蟲穀蠹(Rhyzopertha dominica (Fabricius))、麥蛾(Sitotroga cerealella (Olivier))、粉斑螟蛾(Cadra cautella (Walker))及次級害蟲角胸粉扁蟲(Cryptolestes pusillus Schonherr)、背圓粉扁蟲(Ahasverus advena (Waltl))、茶蛀蟲(Liposcelis divinatorius Müller)等有較佳誘引效果。貯米袋內直接取樣則顯示,此3處常溫倉初級害蟲均以米象(Sitophilus oryzae (L.))為主,與燈光誘引器所誘得之蟲相差異極大,而次級害蟲則與燈光誘引器所誘得之蟲相較一致。此等結果顯示,燈光誘引器在進口糙米倉之害蟲監測應用上,適合做為監測米象以外大部分積穀害蟲消長之工具。 最後,為解決檢疫樣品中常發現害蟲幼體,卻難以從形態鑑定其種類的問題,而開發分子快速鑑定技術。應用序列特徵化增幅區域(sequence characterized amplified region, SCAR)技術,建立3組複合式PCR(Multiplex PCR)鑑定試劑組,分別命名為SCAR-I、-II與-III。每一試劑組內含可同時鑑別外米綴蛾(Corcyra cephalonica (Stainton))、粉斑螟蛾、麥蛾及印度穀蛾(Plodia interpunctella (Hübner))等四種主要鱗翅目積穀害蟲之專一性引子對;其中SCAR-I可分別擴增得205、550、324及382 bp之專一片段,SCAR-II可分別擴增出341、565、261及170 bp片段,SACAR-III可分別擴增得514、555、445及299 bp片段。利用此3組複合式PCR於進口穀物之害蟲樣品,可快速、簡易及精準地鑑定出此四種鱗翅目害蟲之種類,顯示該技術足以開發於進口米檢疫害蟲檢查快速鑑定之應用。
Since Taiwan became a member of the World Trade Organization (WTO) in 2002, the government began to import rice for long-term storage. As a result, monitoring foreign species of insect pests in the imported rice has become one of important matters. The aims of this study were to understand the occurrence of key pests in the imported rice and to set up the methods for monitoring and rapid identification of species of stored product insects. First, a survey conducted during 2003-2006 showed that live insects and mites were intercepted in 12% of the inspected samples of imported brown rice, but none of them were listed as quarantine pests in Taiwan. Eleven species of insects and one species of mite were found after their storage in warehouses. For storage at room temperature for three months, the average number of insects in the brown rice was below 10 insects per kilogram, while the number increased to 35 insects per kilogram after stored for six months. The occurrence of pest insects in low temperature warehouses (15-18°C) was delayed until six months of storage, while the number was below 1 insect per kilogram since 2004. Some stored-product pests can be trapped by light due to their positive phototactic behavior; we therefore further evaluated light trap efficacy to develop methods for monitoring stored product insects. Light traps were used for long-term monitoring in the same batch of USA-imported brown rice stored separately in Linnei, Cihtong (Yunlin County), Liujia (Tainan City), and Renwu (Kaohsiung City). The results of light-trapping in the common storehouses in Linnei, Cihtong, and Liujia showed similar compositions of primary and secondary pests, and have better trapping efficacy on the primary pests of Sitotroga cerealella (Olivier), Rhyzopertha dominica (Fabricius), and Cadra cautella (Walker) and the secondary pests of Cryptolestes pusillus Schonherr, Ahasverus advena (Waltl), and Liposcelis divinatorius Müller. However, the direct survey in stored rice bags showed that Sitophilus oryzae was the key primary pest in these common storehouses, which was different from the light-trapped results. However, the minor species of the secondary pests and their ratio of amount when surveyed by two different methods were similar. These results indicate that light traps used to monitor the population fluctuation of most stored-product pests in imported brown rice are applicable. Finally, insect pests intercepted from imported brown rice are frequently as immature forms, and their morphological identification is difficult. To solve this problem, a DNA identification method based on a sequence-characterized amplified region-polymerase chain reaction (SCAR-PCR) was developed. Here, three sets of multiplex SCAR-PCR mixtures, namely SCAR-I, -II, and -III, were finally developed with each set composed of four species-specific primer pairs derived from the genomic DNA of four major lepidopterous stored-product pests: Corcyra cephalonica (Stainton), C. cautella, S. cerealella, and Plodia interpunctella (Hübner). The SCAR-I amplicons of C. cephalonica, C. cautella, S. cerealella, and P. interpunctella were 205, 550, 324, and 382 bp, respectively, while those of SCAR-II were 341, 565, 261, and 170 bp, and those of SCAR-III were 514, 555, 445, and 299 bp. These multiplex PCR mixtures could sensitively and unambiguously detect and identify individuals among the four lepidopterous pests intercepted in imported stored-products.
URI: http://hdl.handle.net/11455/30693
其他識別: U0005-2004201209451100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2004201209451100
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