請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/30697
標題: Expression of BdmGC-1 in three imaginal discs of the oriental fruit fly, Bactrocera dorsalis (Hendel)
東方果實蠅鳥苷酸環化酶於三種成蟲盤上之表現分析
作者: Chung, Po-Hsu
莊博旭
關鍵字: Bactrocera dorsalis
鳥苷酸環化酶成蟲盤
BdmGC-1
imaginal disc
出版社: 昆蟲學系所
引用: Bredt, DS and Snyder, SH (1994) Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem 63: 175-195. Chang, JC, Yang, RB, Adams, ME and Lu, KH (2009) Receptor guanylyl cyclases in Inka cells targeted by eclosion hormone. Proc Natl Acad Sci U S A 106: 13371-13376. Chang, JC, Yang, RB, Chen, YH and Lu, KH (2006) A novel guanylyl cyclase receptor, BdmGC-1, is highly expressed during the development of the oriental fruit fly Bactrocera dorsalis (Hendel). Insect Mol Biol 15: 69-77. Chiu, HT (1987) Studies on the improvement of mass rearing for oriental fruit flies. Plant Prot Bull 20: 87-92. Davies, SA (2006) Signalling via cGMP: lessons from Drosophila. Cell Signal 18: 409-421. Duda, T and Sharma, RK (2008) ONE-GC membrane guanylate cyclase, a trimodal odorant signal transducer. Biochem Biophys Res Commun 367: 440-445. Ewer, J, Gammie, SC and Truman, JW (1997) Control of insect ecdysis by a positive-feedback endocrine system: roles of eclosion hormone and ecdysis triggering hormone. J Exp Biol 200: 869-881. Fristrom, D (1976) The mechanism of evagination of imaginal discs of Drosophila melanogaster. III. Evidence for cell rearrangement. Dev Biol 54: 163-171. Fristrom, D and Chihara, C (1978) The mechanism of evagination of imaginal discs of Drosophila melanogaster. V. Evagination of disc fragments. Dev Biol 66: 564-570. - 25 - Garcia-Bellido, A and Merriam, JR (1971) Parameters of the wing imaginal disc development of Drosophila melanogaster. Dev Biol 24: 61-87. Gibbs, SM, Becker, A, Hardy, RW and Truman, JW (2001) Soluble guanylate cyclase is required during development for visual system function in Drosophila. J Neurosci 21: 7705-7714. Gibbs, SM and Truman, JW (1998) Nitric oxide and cyclic GMP regulate retinal patterning in the optic lobe of Drosophila. Neuron 20: 83-93. Gibson, MC and Schubiger, G (2001) Drosophila peripodial cells, more than meets the eye? Bioessays 23: 691-697. Guillermet, C and Mandaron, P (1980) In vitro imaginal disc development and moulting hormone. J Embryol Exp Morphol 57: 107-118. Guo, D, Tan, YC, Wang, D, Madhusoodanan, KS, Zheng, Y, Maack, T, Zhang, JJ and Huang, XY (2007) A Rac-cGMP signaling pathway. Cell 128: 341-355. Guo, D, Zhang, JJ and Huang, XY (2010) A new Rac/PAK/GC/cGMP signaling pathway. Mol Cell Biochem 334: 99-103. Jaffe, AB and Hall, A (2005) Rho GTPases: biochemistry and biology. Annu Rev Cell Dev Biol 21: 247-269. Jaffer, ZM and Chernoff, J (2002) p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol 34: 713-717. Kingan, TG, Gray, W, Zitnan, D and Adams, ME (1997) Regulation of ecdysis-triggering hormone release by eclosion hormone. J Exp Biol 200: 3245-3256. Koyama, T, Iwami, M and Sakurai, S (2004) Ecdysteroid control of cell cycle and cellular commitment in insect wing imaginal discs. Mol Cell Endocrinol 213: 155-166. Kuzin, B, Roberts, I, Peunova, N and Enikolopov, G (1996) Nitric oxide regulates cell proliferation during Drosophila development. Cell 87: - 26 - 639-649. Lawrence, PA and Morata, G (1977) The early development of mesothoracic compartments in Drosophila. An analysis of cell lineage and fate mapping and an assessment of methods. Dev Biol 56: 40-51. Lucas, KA, Pitari, GM, Kazerounian, S, Ruiz-Stewart, I, Park, J, Schulz, S, Chepenik, KP and Waldman, SA (2000) Guanylyl cyclases and signaling by cyclic GMP. Pharmacol Rev 52: 375-414. Mentzel, B and Raabe, T (2005) Phylogenetic and structural analysis of the Drosophila melanogaster p21-activated kinase DmPAK3. Gene 349: 25-33. Mitchell, N, Cranna, N, Richardson, H and Quinn, L (2008) The Ecdysone-inducible zinc-finger transcription factor Crol regulates Wg transcription and cell cycle progression in Drosophila. Development 135: 2707-2716. Morton, DB and Simpson, PJ (2002) Cellular signaling in eclosion hormone action. J Insect Physiol 48: 1-13. Oberlander, H, Leach, CE and Shaaya, E (2000) Juvenile hormone and juvenile hormone mimics inhibit proliferation in a lepidopteran imaginal disc cell line. J Insect Physiol 46: 259-265. Pilz, RB and Casteel, DE (2003) Regulation of gene expression by cyclic GMP. Circ Res 93: 1034-1046. Ridley, AJ (2001) Rho GTPases and cell migration. J Cell Sci 114: 2713-2722. Schneeberger, D and Raabe, T (2003) Mbt, a Drosophila PAK protein, combines with Cdc42 to regulate photoreceptor cell morphogenesis. Development 130: 427-437. Sun, L, Wang, H, Hu, J, Han, J, Matsunami, H and Luo, M (2009) Guanylyl cyclase-D in the olfactory CO2 neurons is activated by - 27 - bicarbonate. Proc Natl Acad Sci U S A 106: 2041-2046. Taylor, J and Adler, PN (2008) Cell rearrangement and cell division during the tissue level morphogenesis of evaginating Drosophila imaginal discs. Dev Biol 313: 739-751. Tobler, A and Nijhout, HF (2010) A switch in the control of growth of the wing imaginal disks of Manduca sexta. PLoS One 5: e10723. Truman, JW (1981) Interaction between ecdysteroid, eclosion hormone, and bursicon titers in Manduca sexta. Amer. Zool. 21: 655-661. Truman, JW, Hiruma, K, Allee, JP, Macwhinnie, SG, Champlin, DT and Riddiford, LM (2006) Juvenile hormone is required to couple imaginal disc formation with nutrition in insects. Science 312: 1385-1388. Verma, A, Hirsch, DJ, Glatt, CE, Ronnett, GV and Snyder, SH (1993) Carbon monoxide: a putative neural messenger. Science 259: 381-384. Zitnanova, I, Adams, ME and Zitnan, D (2001) Dual ecdysteroid action on the epitracheal glands and central nervous system preceding ecdysis of Manduca sexta. J Exp Biol 204: 3483-3495.
摘要: Guanylyl cyclases (GCs), kind of enzyme-linked receptor proteins, play important physiological functions, including metamorphosis, olfactory organ and memory, in insects. The BdmGC-1, a receptor form membrane guanylyl cyclase of Bactrocera dosalis (Hendel), has been proved its relationship with eclosion hormone. In this study, we furtherfound that the BdmGC-1 was expressed in the B. dorsalis imaginal discs of wings, legs and compound eyes. Real-time PCR analysis revealed that new (1-day-old) puparium exhibited higher expression level during metamorphosis. Moreover, immunofloruscent staining showed that the BdmGC-1 signals were located in whole imaginal discs. Furthermore, recent studies have showed that p-21 activated protein (PAK) can regulate the mammalian GC activity. Hence, we used a 280-bp BdPAK, cloned from B. dorsalis based on PAK conserved regions, to analyze its expression and location. The results of both real-time PCR analysis and immunofloruscent staining showed that BdPAK has similar expression profiles with BdmGC-1, implying that there some kind of relationships are existed between these two proteins. In summary, BdmGC-1 is indeed expressed in the B. dorsalis wing, compound eye and leg discs and the newly pupated purarium have the higher expression level of BdmGC-1. However, functional interaction of BdmGC-1 with BdPAK has not yet been understood.
鳥苷酸環化酶(guanylyl cyclase, GC)為昆蟲體內一重要受器蛋白(receptor protein),許多生理功能都與其相關,例如變態、嗅覺和記憶等。東方果實蠅(Bactrocera dorsalis (Hendel))鳥苷酸環化酶,BdmGC-1,已被證實為羽化激素(eclosion hormone)之受器蛋白,並發現其可能與昆蟲蛻皮行為有關。本研究更進一步發現BdmGC-1亦存在東方果實蠅幼蟲體內的翅成蟲盤(wing disc)、眼成蟲盤(eye disc)和足成蟲盤(leg disc)上。以real-time PCR 分析發現,蛹期第一天之翅、眼和足成蟲盤均有較高的BdmGC-1 表現量;以免疫螢光染色(immunofluorescent stain)分析BdmGC-1 在翅、眼和足成蟲盤表現位置,結果顯示其分布位於整個成蟲盤上。另外,從前人研究已知p-21 activated protein(PAK)在哺乳動物上可調節GC 活性。因此,以PAK 保守區域選殖得到一長度280 bp 的東方果實蠅部分PAK 序列,且命名為BdPAK。偵測其基因表現量和BdPAK螢光染色之結果顯示,BdPAK 之表現與位置與BdmGC-1 皆類似,表示BdPAK 與BdmGC-1可能有某種關聯。綜合上述結果可知,BdmGC-1 表現在翅、眼和足成蟲盤上並於蛹期第一天有較高表現,且可能與BdPAK 有某種關聯。
URI: http://hdl.handle.net/11455/30697
其他識別: U0005-2108201118190000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2108201118190000
顯示於類別:昆蟲學系

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。