請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/31004
標題: 建立快速篩檢植物基質中酵母菌多樣性之鑑定資料庫
Establishment of the identification database for the rapid survey of yeast diversity on plant materials
作者: 林利娜
Lin, Li-Na
關鍵字: Yeasts
酵母菌
diversity
database
search system
多樣性
資料庫
搜尋系統
出版社: 植物病理學系所
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摘要: 酵母菌廣泛分布於自然環境中,於不同環境基質上所存在之多樣性酵母菌物種,皆衍生出特有之性質,可作為開發其應用價值之基礎。探索酵母菌在環境中之多樣性,將可找到更多有益菌種,供未來永續利用。本研究調查植物基質中酵母菌之多樣性,探討不同基質上酵母菌物種之差異。酵母菌多樣性研究,先對所蒐集之菌株分析限制酵素片段長度多型性(RFLPs),依DNA圖譜歸納分群,再從各群中選擇代表菌株以 DNA 序列比對鑑定。為了改良現有多樣性調查方法,本研究發展酵母菌資料庫搜尋系統,將RFLPs圖譜儲存在資料庫中,結合網路搜尋功能,讓多樣性研究過程中菌種之蒐集整理與鑑定能更有效率及精確。本研究蒐集 339 個菌株,共有74 種,包括 18 個新種,並將資料庫搜尋結果和圖鑑檔案結合。透過持續新增新的菌種資訊,未來在多樣性調查時,任何蒐集到的菌株皆能快速的進行初步之鑑定,而資料庫搜尋系統可做為管理菌種及發現新的物種的有利工具。
Yeasts are ubiquitous in the ecosystem. Diverse species are derived from diverse environments and substrates. In order to adapt to different habitats, numerous properties have been evolved in different yeasts. They are potentially useful for human being. The objective of this study was to survey the diversity of yeasts from different plant materials, and to realize the correlation between substrates and yeasts species. During the survey of yeast diversity, a lot of isolates will flood in. Therefore, to separate all the obtained isolates into groups by RFLPs fingerprinting is essential. In order to develop a proper method to manage the DNA fingerprinting data derived from RFLPs, a search system combined with database was designed in this study. All the DNA fingerprinting data are stored in database and can be retrieved through designed webpage. This dadabase search system will make the organization and identification of yeast isolates efficient and accurate. There were 339 isolates obtained in this study. They can be attributed to 74 species, including 18 new species. Photos containg all the discernable characters were established for all the obtained species and they were all deposited in the database. Further species or isolate with fingerprinting data can continuously be added to this database to complete this database. It is expected that this database search system will be a helpful and powerful tool to manage yeast culture collections and to explore new species in the future.
URI: http://hdl.handle.net/11455/31004
其他識別: U0005-1908201115285800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1908201115285800
顯示於類別:植物病理學系

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