Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31028
標題: 四季海棠輪紋病之病因及病原特性
Etiology of a begonia ringspot disease and characterization of its causal agent
作者: 洪郁豪
Hong, Yu-Hau
關鍵字: Begonia semperflorens
四季海棠
Zucchini yellow mosaic virus
cross protection
矮南瓜黃化嵌紋病毒
交互保護
出版社: 植物病理學系所
引用: 許秀惠、王惠亮、黃秋雄‧1985‧矮南瓜 (Zucchini) 黃化嵌紋病 毒之分離與鑑定‧中華農業研究34 (1) : 87-95。 張清安、柯南靖‧1982‧感染絲瓜之新病毒—絲瓜嵌紋病毒之鑑定‧植保會刊24 (4) : 281。 楊秀珠‧1990 ‧台湾産Colletotrichum菌による植物の炭疽病について‧北海道大學農學院農學博士論文‧208頁。 劉嵋恩‧1982‧臺北市花卉病害研究調查。 鄭元春、鄭元鑫‧1993 ‧花卉天地‧臺灣省立博物館印行‧臺灣省台北市。. 9-11. 謝煥儒‧1983‧臺灣植物白粉病之調查(一) ‧林試所試驗報告 383: 1-14。 Albouy, J., 1995. Begonia. Pages 449-454 In: Loebenstein G, Lawson RH, Brunt AA, eds. Virus and Virus - like Diseases of Bulb and Flower Crops. John Wiley & Sons. Chichester.UK. 543 pp. Aleman-Verdaguer, M. E., Goudou-Urbino, C., Dubern, J., Beachy, R. N., and Fauquet, C. 1997. Analysis of the sequence diversity of the P1, HC, P3, NIB and CP genomic region of several Yam mosaic potyvirus isolates; implications for the intraspecies molecular diversity of potyvirus. J. Gen. Virol. 78: 1253-1264. Allison, R., Johnston, R. E., and Dougherty, W. G. 1986. The nucleotide sequence of the coding region of Tobacco etch virus genomic RNA evidence for the synthesis of a single polyprotein. Virology 154: 9-20. Anandalakshmi, R., Pruss, G. J., Ge, X., Marathe, R., Mallory, A. C., Smith, T. H., and Vance, V. B. 1998. A viral suppressor of gene silencing in plants. Proc. Natl. Acad. Sci. USA 95: 13079-13084. Baker, C. A., Hiebert E., Marlow G. C., and Wisler G. C. 1992. Comparative sequence analysis of the Reunion isolate of zucchini yellow mosaic virus. Phytopathology 82: 1176. Balint, R., Plooy, I., and Steele, C. 1990. The nucleotide sequence of zucchini yellow mosaic potyvirus. Abstract of the VIIIth International Congress of Virology 8: 84-107. Blua, M. J., and Perring, T. M., 1992. Effects of zucchini yellow mosaic virus on colonization and feeding behavior of Aphis gossypii (Homoptera: Aphididae) alatae. Environmental Entomology 21: 578-585. Christie, S.R., Purcifull, D.E., Crawford, W.E., and Ahmed, N.A. 1985. Electron microscopy of negatively stained clarified viral concentrates obtained from small tissue samples with appendices on negative staining techniques. Fla. Agr. Sta. Bull. No. 872. 45 pp. Desbiez, C., Wipf-Scheibel, C., Granier, F., Robaglia, C., Delaunay, T., Lecoq, and H. 1996. Biological and molecular variability of zucchini yellow mosaic virus on the island of Martinique. Plant Dis. 68: 537. Dijkstra, J., and de Jager, C. P. 1998. Practical Plant Virology - Protocols and Exercises. Springer-Verlag, Berlin. 459 pp. Edwardson, J. R. 1974. Some properties of the potato virus Y group. Florida Experimental Station Monograph. Series. No. 4, IFAS, Gainesville, 398 pp. Farr, D. F., Bills, G. F., Chamuris, G. P., and Rossman, A.Y. 1989. Fungi on plants and plant products in the United States. American Phytopathological Society, St. Paul, Minnesota, USA. 592-593. Fauquet, C. M., Mayo, M. A. Maniloff, J., Desselberger, U., and Ball, L. A. 2005. Virus Taxonomy – Eighth Report of the International Committee on the Taxonomyof Viruses. Elsevier Academic Press. 1259 pp. German, T. L. 1992. Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu. Rev. Phytopathol. 30: 315-348. Harlow, E., and Lane, D. 1988 Labeling antibodies. In: Antibodies: A Laboratory Manual. E. Harlow and D. Lane, eds., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 319–358. Hiebert, E., Pucifull, D. E. and Christie, R. G. 1984. Purification and immunological analysis of plant viral inclusion bodies. pp. 225-280 In K. Maramorosch and H. Koprowski, eds., Methods in Virology, Academic Press, USA. Hsu, C. H., Lin, S. S., Liu, F. L., Su, W. C., and Yeh, S. D. 2004. Oral administration of a mite allergen expressed by Zucchini yellow mosaic virus in cucurbit species downregulates allergen-induced airway inflammation and IgE synthesis. J. Allergy Clin. Immunol. 113:1079-1085. Kasschau, K. D., and Carrington, J. C. 1998. A counterdefensive strategy of plant viruses; Suppression of posttranscriptional gene silencing. Cell 95: 461-470. Kekarainen, T., Merits, A., Oruetxebarria, I., Rajamaki, M. L., and Valkonen, V. J. P. T. 1999. Comparison of the complete sequences of five different isolates of potato virus A (PVA), genus potyvirus. Arch. Virol. 144: 2355-2366. Kiyokawa, S., Kikuchi,Y., Kamada, H. and Harada, H. 1996. Genectic transformation of Begonia tuberhybrida by Ri rol genes. Plant cell reports. Vol. 15. 8:606-609. Koenig, R. 1981. Indirect ELISA methods for the broad specificity detection of plant viruses. J. Gen. Virol. 55: 53-62. LaMondia, J. A. 1995. First report of the foliar nematode Aphelenchoides fragariae infecting lamium. Plant Dis. 80: 203-207. Langeveld, S. A., Dore, J. M., Memelink, J., Derks, A. F. L. M., Vlugt, C. I. M. van der, Asjes, C., Bol, J. F. 1991. Identification of potyviruses using the polymersae chain reaction with degenerate primers. J. Gen. Virol. 72: 1531-1541. Lecoq, H., Lemaire, J. M., and Wipf-Scheibel, C. 1991 Control of Zucchini yellow mosaic virus in squash by cross protection. Plant Dis. 75: 208-211. Lecoq, H., Pitrat M, Clement M, 1981. Identification et caracterisation d’ un potyvirus provoquant la maladie du rabougrissement jaune du melon. Agronomie 1: 827-834. Lim, S. T., Wong, S. M., Yeong, C.Y., Lee, S. C., and Goh, C. J. 1993. Rapid detection of cymbidium mosaic virus by the polymerase chain reaction. J. Virol. Methods 41 : 37-46. Lin, S. S., Hou, R. F., Huang, C. H., and Yeh, S. D. 1998. Characterization of Zucchini yellow mosaic virus (ZYMV) isolates collected from Taiwan by host reactions, serology, and RT-PCR. Plant Prot. Bull. 40: 163-176. Lin, S. S., Hou, R. F., and Yeh, S. D. 2000. Heteroduplex mobility and sequence analyses for assessment of variability of Zucchini yellow mosaic virus. Phytopathology 90: 228-235. Lin, S. S., Hou, R. F., and Yeh, S. D. 2001 Complete genome sequence and genetic organization of a Taiwan isolate of Zucchini yellow mosaic virus. Bot. Bull. Acad. Sin. 42:243-250 Lin, S. S., Wu, H. W., Jan, F. J., Hou, R. F. and Yeh, S. D. 2007. Modifications of the helper component-protease of Zucchini yellow mosaic virus for generation of attenuated mutants for cross protection against severe infection. Phytopathology 97, 3: 287-296. Lisa, V., Boccardo, G, D’Agostino, G., Dellavalle, G., and D’Aquilio, M. 1981. Characterization of a potyvirus that causes zucchini yellow mosaic. Phytopathology 71: 667-672. Lisa, V., and Lecoq, H. 1984. Zucchini yellow mosaic virus. CMI/AAB Descriptions of Plant Viruses. No. 282. Kew. Surrey. Lockhart, B.E. 1982. Broad bean wilt in begonia in Minnessota. Plant Dis. Reptr. 66: 72-73. Lockhart, B.E. and Betzold, J.A. 1979. Begonia yello spot: a disease caused by tobacco ringspot virus infection. Plant Dis. Reptr. 63: 1046-1047. Nameth, S.T., Dodds, J.A., Paulus, A.O., and Kishaba, A. 1985. Zucchini yellow mosaic virus associated with severe diseases of melon and watermelon in southeastern California desert valleys. Plant Dis. 69: 785-788. Paludan, N. and Begtrup, J. 1985. Carnation mottle virus demonstrated in Begonia elatior and Begonia x Chelimantha showing vein clearing, leaf curl and flower break. Acta Hortic. 164: 33-39. Provvidenti, R., Gonsalves, D., and Humaydan, H.S. 1984. Occurrence of Zucchini yellow mosaic virus in cucurbits from Connecticut. New-York. Florida. and California. Plant Dis. 68: 443-446. Riechmann, J. L., Lain, S., and Garcia, J. A. 1991. Identification of initiation codon of the Plum pox potyvirus genomic RNA. Virology 185:554-552. Riechmann, J. L., Cervera, M. T., and Garcia, J. A. 1995. Progressing of the Plum pox virus polyprotein at the P3-6K1 junction is not required for virus viability. J. Gen. Virol. 76: 951-956. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17: 208-212. Samyn, G. and Welvaert, W. 1987. Improvement of quality and profit-earning capacity with virus-free culture of Begonia x tuberhybrida multiflora group. Med. Fac. Landbouw., RUG 52: 1059-1064. Sawada K., 1959. Descriptive catalogue of. Taiwan (Formosan) fungi. Part XI. Spe-. cial Publ. Coll. Agric. Nat1. National. Taiwan University. 268pp. Schokthof, H.B., Scholthof, K.B.G., and Jackson, A.O. 1996. Plant virus gene vectors for transient expression of foreign protein in plants. Annu. Rev. Phytopathol. 34: 299-323. Taylor, W. M., and Hagerman, P. J. 1987. A general methods for cloning DNA fragments in multiple copies. Gene 53: 139-144. Varrelmann, M., Maiss, E., Pilot, R.and Palkovics, L. 2007. Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing. J. Gen. Virol. 88: 1005-1015. Verma, N., Hallan, V., Ram, R. and Zaidi, A.A. 2002. Detection of Prunus necrotic ringspot virus in begonia by RT-PCR. Plant Pathol. 51: 800. Ward, C. Q., and Shukla, D. D. 1991. Taxonomy of potyviruses current problems and some solutions. Intervirology 32: 269-296. Welvaert, W., Samyn, G. and van Wijmersch, E. 1980. On the production of virus-free Begonia tuberhybrida cv. multiflora varieties. Acta Hortic. 110: 253-257. Yeh, S.D. 1994. Comparison of the genetic organization of papaya ringspot virus with other potyvirus. Plant Pathology Bull. 3: 54-64. Zhao, M. F., Chen, J., Zheng, H. Y., Adams, M. J. and Chen, J. P. 2003. Molecular analysis of Zucchini yellow mosaic virus isolates from Hangzhou, China. J. Phytopathol. 151: 307-311.
摘要: 四季海棠 (Begonia semperflorens Link & Otto.) 是一種常見的海棠科(Begoniaceae) 盆花植物,為多年生草本。2006 年 5 月在中興大學園藝系的溫室中四季海棠植株上,發現疑似病毒感染所引起的病徵,葉片出現黃色輪紋狀病斑,病斑密集處呈黃化區塊。機械接種於奎藜 (Chenopodium quinoa) 7-10 天後接種葉產生黃化單斑。單離的病毒回接於四季海棠 10 天後,新葉產生黃化輪斑紋點的病徵,證實該單離的病毒分離株為四季海棠輪紋病之病原。病組織粗汁液經電子顯微鏡鏡檢,可觀察到大小約為 750 x 13 nm的長絲狀病毒顆粒,並且在超薄切片的實驗中,在寄主植物細胞內觀察到典型 potyviruses 的病毒內含體。經血清學及分子檢測技術,包括酵素連結免疫吸附分析反應 (ELISA)、西方轉漬法 (Western blot) 和反轉錄聚合酶鏈鎖反應 (RT-PCR) 等方法檢測,證實該單離的病毒為矮南瓜黃化嵌紋病毒 (Zucchini yellow mosaic virus, ZYMV) 的四季海棠分離株,以 ZYMV-Bego為其代稱。ZYMV-Bego的寄主範圍與本土的矮南瓜分離株-ZYMV-TW-TN3 (AF127929) 類似,惟在個別寄主上引起的病徵較 ZYMV-TW-TN3 所引起者輕微。本研究結果顯示四季海棠為 ZYMV 的天然寄主之一,此發現為一新記錄。以已發表的 ZYMV-SG (AJ316228) 序列做為參考設計引子對,ZYMV-Bego 的核酸序列除 3′ poly(A) 外已完成解序 (9591 nt),其中具有單 1 個開放讀架 (ORF),為一聚合蛋白 (polyprotein),可裂解成10 個蛋白質,由 5´ 端依照順序分別為 P1、HC-Pro、P3、6K1、CI、6K2、VPg、NIa-Pro、Nib 和 CP。將所得的核酸序列與 NCBI 資料庫已登錄的 13 個 ZYMV 病毒株比對後可發現,ZYMV-Bego 的核酸序列與其他 ZYMV 的核酸相同度介於 92-98 % 之間,而與亞洲地區發現的ZYMV分離株,如 ZYMV-CU (AJ307036)、ZYMV-SG (AJ316228) 及ZYMV-TW-TN3 (AF127929) 等分離株最為相似。比較病毒的每一個單一開放讀架的序列後可知,序列差異度最大者為 P1 基因,最小者為 NIa-Pro 基因。由於ZYMV-Bego 在瓜類作物上引起之病徵較ZYMV-TW-TN3 來得輕微,因此進行應用ZYMV-Bego於交互保護可行性的測試。
Begonia semperflorens Link & Otto is one kind of common pot plant belonging to Begoniaceae family. Some begonia plants with symptoms of chlorotic ringspot on leaves were found in one of the greenhouses at the National Chung Hsing University, Taichung, in the spring of 2006. On some severely infected begonia plants, several ringspots fused together and became a coalesced chlorotic region on leaves. Isolation of the virus was conducted by single-lesion transfer on Chenopodium quinoa by mechanical inoculation. Several chlorotic local lesions appeared 7-10 days after inoculation. Back inoculation with an isolated virus to healthy begonia confirmed the pathogenicity of the virus to begonia, as reflected in several chlorotic ringspots on showing upper leaves 10 days after inoculation. Electron microscopic examinations revealed virus particles of flexuous-rods with a size of 750×13 nm in the preparations of infected crude saps and purified virus suspensions. Typical inclusion bodies of potyviruses were also observed in ultrathin sections of infected tissues from begonia, squash and C. quinoa. Serological tests, including indirect-ELISA and western blotting, indicated that the isolated virus was serologically related to Zucchini yellow mosaic virus (ZYMV), which has never been reported to be a pathogen to begonia, and thus was designated as an begonia isolate of ZYMV, ZYMV-Bego. Host range tests and symptom comparison between ZYMV-Bego and ZYMV-TW-TN3 showed that Bego is a mild strain of ZYMV. ZYMV primers designed from genomic sequence of ZYMV-SG were used to amplify cDNA fragments and sequence the corresponding clones to viral RNA of this virus. Full-length sequence sequencing except 3′ poly(A) has been complete (9591 nt), and it has only one open reading frame which translates for a polyprotein. The polyprotein can be cleaved in 10 functional proteins. They are P1, HC-Pro, P3, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and CP at the order from 5′ end. The sequencing results were submitted to NCBI database for nucleotides identification. Based on the identification results, this virus is identified as a new ZYMV isolate that similar to ZYMV-CU (AJ307036)、ZYMV-SG (AJ316228) and ZYMV-TW-TN3 (AF127929) which are originally found in Asia. Since the ZYMV-Bego is considered as a mild strain of ZYMV, the possibility of applying it in cross protection is tested.
URI: http://hdl.handle.net/11455/31028
其他識別: U0005-2208200715214600
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