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Application of Antagonistic Bacteria for Controlling Clubroot of Crucifers
對根瘤病菌（Plasmodiophora brassicae Wor.）的游走孢子侵入根毛較
x103 cfu／g wet stem，而73R及80S的菌量都在104 cfu／g wet stem以
wet root 以上，而10S的菌量最低，大約1.01x103 cfu／g wet root。而
小，分別為9.24x103, 2.31x104 及 3.47x104 cfu／g wet root 左右。|
The purpose of this study is to use endophytic and root colonized antaists to control club root disease of crucifers. One hundred and fifteen bacterial strains were isolated either from healthy or slightly diseased cabbage root tissues collected from Ching-jing area. According to analysis of antagonistic ability of these 115 strains against infection of root hairs by Plasmodiophora brassicae, seventeen strains exhibiting the inhibition ability on infection of root hairs in 10 days were selected to evaluate the control ability in pots after six weeks. The results revealed that three out of seventeen strains decreased club root disease index. These three antagonistic strains 10, 73 and 80 were identified as Bacillus spp. and were used as biocontrol agents in further study. Before transplanting the seedlings to infested soil infected with107 spores/g soil, two pretreatments of the seedlings with these antagonistic strains were perfomed. That was, the 10-day-old seedlings with or without cutting at hypocotyl were dipped into antagonistic bacteria suspension (108 cfu/ml) for 12 hours. All three strains of antagonistic bacteria tested reduced club root disease index significantly. The results of population dynamic assay revealed that the population of antigonists declined along with plant growth. In the case the plants have been treated by cutting the seedlings at hypocotyl and dipping into antagonistic bacteria suspension, the population of each strain in stem was declined from 105~106 to 103~104 cfu/g wet stem, and was declined from 106 to 103~105 cfu/g wet root in root during eight weeks period of treatments. Population of all strains in the rhizosphere were declined from 106 to 103~104 cfu/g wet root by dipping root into antagonistic bacteria suspension during eight weeks period of treatments.
|Appears in Collections:||植物病理學系|
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