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標題: 利用拮抗細菌防治十字花科蔬菜根瘤病
Application of Antagonistic Bacteria for Controlling Clubroot of Crucifers
作者: 陳俊欽
Chen, Chun-Chin
關鍵字: antagonist
出版社: 植物病理學系
摘要: 中文摘要 由清境農場高冷蔬菜區中發病田中取回不發病或發病輕微 的甘藍根部組織,分得115支細菌菌株。將甘藍幼苗以胚軸切斷接種法及 浸根法接種前述的細菌後,再種至根瘤病的導病土中,以根毛感染法篩選 對根瘤病菌(Plasmodiophora brassicae Wor.)的游走孢子侵入根毛較 具抗性的細菌菌株。以根毛感染法所篩到的菌株分別以胚軸切斷和浸根接 種法去做盆栽防治試驗,發現二種不同的處理,得到的結果相同,同是編 號10,73、80三支同屬於Bacillus屬的細菌拮抗效果較佳。以胚軸切斷接 種法處理者,這三支菌分別可將根瘤病的病害指數由87降至65、58及65; 以浸根接種法處理者,這三支菌分別可將根瘤病的病害指數由84降至52 、46及35。為偵測拮抗細菌在甘藍植株體內及根圈的族群動態,以抗生素 rifampicin及streptomycin篩選抗生素抗性的拮抗菌株,記作10S,73R 及80S供做試驗菌株。以胚軸切斷接種法接種拮抗細菌後,至第八週在植 物莖部組織內均都可偵測到拮抗菌的存在,以10S的菌量最低,大約6.56 x103 cfu/g wet stem,而73R及80S的菌量都在104 cfu/g wet stem以 上;在根部組織,接種八週後,以73R的含量最高,大約3.52x105 cfu/g wet root 以上,而10S的菌量最低,大約1.01x103 cfu/g wet root。而 以浸根接種法處理者,第八週後,這三支菌在甘藍根圈的族群數目差異較 小,分別為9.24x103, 2.31x104 及 3.47x104 cfu/g wet root 左右。
The purpose of this study is to use endophytic and root colonized antaists to control club root disease of crucifers. One hundred and fifteen bacterial strains were isolated either from healthy or slightly diseased cabbage root tissues collected from Ching-jing area. According to analysis of antagonistic ability of these 115 strains against infection of root hairs by Plasmodiophora brassicae, seventeen strains exhibiting the inhibition ability on infection of root hairs in 10 days were selected to evaluate the control ability in pots after six weeks. The results revealed that three out of seventeen strains decreased club root disease index. These three antagonistic strains 10, 73 and 80 were identified as Bacillus spp. and were used as biocontrol agents in further study. Before transplanting the seedlings to infested soil infected with107 spores/g soil, two pretreatments of the seedlings with these antagonistic strains were perfomed. That was, the 10-day-old seedlings with or without cutting at hypocotyl were dipped into antagonistic bacteria suspension (108 cfu/ml) for 12 hours. All three strains of antagonistic bacteria tested reduced club root disease index significantly. The results of population dynamic assay revealed that the population of antigonists declined along with plant growth. In the case the plants have been treated by cutting the seedlings at hypocotyl and dipping into antagonistic bacteria suspension, the population of each strain in stem was declined from 105~106 to 103~104 cfu/g wet stem, and was declined from 106 to 103~105 cfu/g wet root in root during eight weeks period of treatments. Population of all strains in the rhizosphere were declined from 106 to 103~104 cfu/g wet root by dipping root into antagonistic bacteria suspension during eight weeks period of treatments.
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