Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31190
標題: 香蕉黃葉病菌生理小種第四型與西瓜蔓割病菌兩大重要病原菌之分子檢測技術研發
Development of molecular methods for the detection of Fusarium oxysporum f. sp. cubense race 4 and F. oxysporum f. sp. niveum
作者: 林盈宏
Lin, Ying-Hong
關鍵字: Fusarium oxysporum
尖鐮孢菌
Fusarium oxysporum f. sp. cubense
Fusarium oxysporum f. sp. niveum
race
molecular detection
香蕉黃葉病菌
西瓜蔓割病菌
生理小種
分子檢測
出版社: 植物病理學系所
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摘要: 尖鐮孢菌 (Fusarium oxysporum) 可造成多種作物的萎凋病,依其感染寄主作物的不同而可區分成不同分化型 (forma specialis, f. sp.)。例如西瓜蔓割病與香蕉黃葉病即分別由 F. oxysporum f. sp. niveum 與 F. oxysporum f. sp. cubense 所引起。快速且正確的病害診斷法乃作物病害綜合管理的基石。為加速病原菌檢測時程與提高檢測時的靈敏度,我們利用聚合酵素連鎖反應 (polymerase chain reaction) 開發出分別針對香蕉黃葉病菌與西瓜蔓割病菌之快速分子檢測技術。本論文已成功開發出分別對香蕉黃葉病菌生理小種第四型 (race 4) 與西瓜蔓割病菌等尖鐮孢菌具專一性的引子對 (分別命名為 Foc-1/Foc-2 與 Fon-1/Fon-2)。上述引子對搭配最佳化的聚合酵素連鎖反應條件,只需少量待測病原菌及植物檢體即可快速區分出「香蕉黃葉病菌生理小種第四型」與「西瓜蔓割病菌」等具重大危害之「尖鐮孢菌」。
Fusarium oxysporum (Fo) is the causal agent of Fusarium wilt disease of many crops. Fo has several specialized forms (known as forma specialis, f. sp.) such as F. oxysporum f. sp. niveum (Fon) on watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) and F. oxysporum f. sp. cubense (Foc) on banana (Musa spp.). Rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. In order to accelerate the detection process and to increase the sensitivity of detection strategies, we have developed reliable polymerase chain reaction (PCR) techniques to detect Foc and Fon isolates individually in Taiwan. In the PCR assay, the primer sets Foc-1/Foc-2 and Fon-1/Fon-2 produced DNA fragments which are specific to Foc race 4 and Fon, respectively. With the optimized PCR, the molecular method could not only directly detect small quantities of Fo but also tag diseased host samples. We also demonstrated that by using our PCR assay with Fon-1/Fon-2 and Foc-1/Foc-2 primer sets, Fon and Foc race 4 isolates could be easily distinguished from other formae speciales of F. oxysporum, respectively.
URI: http://hdl.handle.net/11455/31190
其他識別: U0005-0910200810210500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0910200810210500
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