請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/31234
標題: Bacillus mycoides、水楊酸類似物及 harpin 蛋白對萵苣幼苗生長及抗病反應的效果
Effects of Bacillus mycoides, SA analogue, and harpin on the growth and disease-resistant response of lettuce seedlings
作者: 陳和緯
Chen, Her-Wei
關鍵字: Plant growth-promoting rhizobacteria
Bacillus mycoides
Fusarium wilt of lettuce
Messenger STS
出版社: 植物病理學系所
引用: 丁姵分. 2006. 番茄萎凋病之生物防治菌的鑑定與防病潛力評估. 國立中興大學植物病理學系碩士論文. 64 pp. 林盈宏、張碧芳. 2008. 阿拉伯芥之NPR1蛋白在植物誘導性系統抗病反應中所扮演的角色. 植病會刊. 17:69-78. 陳盈如. 2006. 辣椒品系 Capsicum baccatum PBC81 及 Capsicum chinense PBC932 對辣椒炭疽病菌 Colletotrichum acutatum 之抗病性初探. 國立中興大學植物病理學系碩士論文. 71 pp. 高德錚. 1996. 生菜萵苣知多少. 台中區農業專訊 15:25-27. 彭玉湘、黃振文. 1997. 台灣萵苣萎凋病之發生. 植保會刊. 39:339. (摘要) 彭玉湘、黃振文. 1998. 萵苣萎凋病菌的病原性測定. 植病會刊. 7:121-127. 黃久菱. 2005. 萵苣萎凋病菌的生理小種鑑定與防治試驗. 國立中興大學植物病理學系碩士論文. 57 pp. 黃玉瓊、黃義弘、陳漢祥、范國洋 編. 1997. 蔬菜病蟲害綜合防治專輯. 行政院農業委員會、台灣省政府農林廳編印. 台北,422 pp. 黃振文. 1991. 利用土壤添加物防治作物之土壤傳播性病害. 植保會刊. 28:81-90. 黃晉興、羅朝村. 1998. 台灣萵苣萎凋病之調查初報. 植病會刊 7:150-153. 劉昱志. 2000. 應用自由基誘導物 2,2’-Azobis (2-amidinopropane) hydrochloride (AAPH) 模擬氧化逆境:研究大豆之抗氧化劑的生化防禦系統. 國立中央大學生命科學系碩士論文. 57pp. Ahmad, F., Ahmad, I., and Khan, M. S. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 163:173-81. Bapat, S., and Shah, A. K. 2000. Biological control of fusarial wilt of pigeon pea by Bacillus brevis. Can. J. Microbiol. 46:125-132. Bargabus, R. L., Zidack, N. K., Sherwood, J. W., and Tacobsen, B. J. 2002. Characterization of systemic resistance in sugar beet elictied by a non-pathogenic, phyllosphere-colonizing Bacillus mycoides, biological control agent. Physiol. Mol. Plant Pathol. 61:289-298. Benhamou, N., Kloepper, J. W., Quadt-Hallmann, A., and Tuzun, S. 1996. Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Physiol. Plant Pathol. 112:919-929. Benhamou, N., and Bélanger, R. R. 1998a. Induction of systemic resistance to Pythium damping-off in cucumber plants by benzothiadiazole: ultrastructure and cytochemistry of the host response. Plant J. 14: 13-21. Benhamou, N., and Bélanger, R. R. 1998b. Benzothiadiazole-mediated induced resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato. Plant Physiol. 118: 1203-1212. Benhamou, N., Kloepper, J. W., and Tuzun, S. 1998. Induction of resistance against Fusarium wilt of tomato by combination of chitosan with an endophytic bacterial strain: ultrastructure and cytochemistry of the host response. Planta 204:153-168. Bloemberg, G. V., and Lugtenberg, B. J. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr. Opin. Plant Biol. 4:343-350. Bottini, R., Cassán, F., and Piccoli, P. 2004. Gibberellin production by bacteria and its involvement in plant growth promotion and yield increase. Appl. Microbiol. Biotechnol. 65:497-503. Cappuccino, J. C., and Sherman, N. 1992. Microbiology: A Laboratory Manual, 3rd edition. Benjamin/cummings Pub. Co. Press. NY., USA. Castro-Sowinski, S., Herschkovitz, Y., Okon, Y., and Jurkevitch, Y. 2007. Effects of inoculation with plant growth-promoting rhizobacteria on resident rhizosphere microorganisms. FEMS Microbiol. Lett. 276:1-11. Chakraborty, U., Chakraborty, B., and Basnet, M. 2006. Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium. J. Basic Microbiol. 46:186-195. Czaban, J., Księżniak, A., and Perzyński, A. 2004. An attempt to protect winter wheat against Fusarium culmorum by the use of rhizobacteria Pseudomonas fluorescens and Bacillus mycoides. Pol. J. Microbiol. 53:175-182. Degl’innocenti, E., Guidi, L., Pardossi, A., and Tognoni, F. 2005. Biochemical study of leaf browning in minimally processed leaves of lettuce (Lactuca sativa L. Var. Acephala). J. Agric. Food Chem. 53:9980-9984. Dobbelaere, S., Vanderleyden, J., and Okon, Y. 2003. Plant growth promoting effects of diazotrophs in the rhizosphere. CRC Crit. Rev. Plant Sci. 22:107-149. Dong, H., Delaney, T. P., Bauer, D. W., and Beer, S. V. 1999. Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene. Plant J. 20:207-215. Driks, A. 2004. The Bacillus spore coat. Phytopathology 94:1249-1251. Durrant, W. E., and Dong, X. 2004. Systemic acquired resistance. Annu. Rev. Phytopathol. 42:185-209. Elizabeth, A. B. E., and Handelsman, J. 1999. Biocontrol of plant disease: a (Gram-) positive perspective. FEMS Microbiol. Lett. 171:1-9. Fujita, N., Tanaka, E., and Murata, M. 2006. Cinnamaldehyde inhibits phenylalanine ammonia-lyase and enzymatic browning of cut lettuce. Biosci. Biotechnol. Biochem. 70:672–676. Gardener, B. B. M., and Driks, D. 2004. Overview of the nature and application of biocontrol microbes: Bacillus spp. Phytopathology 94:1244. Glick, B. R., and Bashan, Y. 1997. Genetic manipulation of plant growth-promoting bacteria to enhance biocontrol of phytopathogens. Biotechnol. Adv. 15:353-378. Gordon, S. A., and Weber, R. P. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiol. 26:192-195. Görlach, J., Volrath, S., Knauf-Beite, G., Hengy, G., Beckhove, U., Kogel, K., Oostendorp, M., Staub, T., Ward, E., Kessmann, H., and Ryals, J. 1996. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629-643. Hukkanen, A. T., Kokko, H., Buchala, A. J., Mcdougall, G. J., Stewart, D., Kärenlampi, S. O., and Karjalainen, R.O. 2007. Benzothiadiazole induces the accumulation of phenolics and improves resistance to powdery mildew in strawberries. J. Agric. Food Chem. 55:1862-1870. Idris, E. E. S., Bochow, H., Ross, H., and Borriss, R. 2004. Use of Bacillus subtilis as biocontrol agent. VI. Phytohormone like action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45 and Bacillus subtilis FZB37. J. Plant Dis. Prot. 111:583-597. Iriti, M., Rossoni, M., Borgo, M., and Faoro, F. 2004. Benzothiadiazole enhances resveratrol and anthocyanin biosynthesis in grapevine, meanwhile improving resistance to Botrytis cinerea. J. Agric. Food Chem. 52:4406-4413. Jang, Y. S., Sohn, S. I., and Wang, M. H. 2006. The hrpN gene of Erwinia amylovora stimulates tobacco growth and enhances resistance to Botrytis cinerea. Planta. 223: 449-456. Johnston, A., and Booth, C. 1983. Plant Pathologist’s Pocketbook, 2nd edition. Commonwealth Mycological Institute Press. England. Joo, G. J., Kim, Y. M., Kim, J. T., Rhee, I. K., Kim J. H., and Lee, I. J. 2005. Gibberellins-producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers. J. Microbiol. 43:510-515. Kim, J. F., and Beer, S. V. 2000. Fire Blight and Its Cansative Agent, Erwinia amylovora. Vanneste, J. L. ed. CAB International Wallingford Press. UK. Kohler, A., Schwindling, S., and Conrath, U. 2002. Benzothiadiazole-induced priming for potentiated responses to pathogen infection, wounding, and infiltration of water into leaves requires the NPR1/NIM1 gene in Arabidopsis. Plant Physiol. 128:1046-1056. Lawton, K. A., Friedrich, L., Hunt, M., Weymann, K., Delaney, T., Kessmann, H., Staub, T., and Ryals, J. 1996. Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J. 10:71-82. Lingappa, Y., and Lookwood, J. L. 1962. Chitin medium for selective isolation and culture of actinomycetes. Phytopathology 52:317-323. Lorck, H. 1948. Production of hydrocyanic acid by bacteria. Physiol Plant 1:142-146. Matuo, T., and Motohashi, S. 1967. On Fusarium oxysporum f. sp. lactucae n.f. causing root rot of lettuce. Trans. Mycol. Soc. Jpn. 32: 13-15. Nash, S. N., and Snyder, W. C. 1962. Quantitative estimation by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52:567-572. Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J., and Setlow, P. 2000. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. Rev. 64:548-572. Pikovskaya, R. E. 1948. Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Microbiologia 17:362-370. Podile, A. R., and Kishore, A. K. 2006. Plant-Associated Bacteria. Gnanamanickam S. S. ed. Springer Press. Netherlands. Thangavelu, R., Palaniswami, A., Doraiswamy, S., and Velazhahan, R. 2003. The Effect of Pseudomoas fluorescens and Fusarium oxysporum f.sp. cubense on induction of defense enzymes and phenolics in banana. Biol. Plant. 46:107-112. Ryu, C. M., Murphy, J. F., Mysore, K. S., and Kloepper, J. W. 2004a. Plant growth-promoting rhizobacteria systemically protect Arabidopsis thaliana against Cucumber mosaic virus by a salicylic acid and NPR1-independent and jasmonic acid-dependent signaling pathway. Plant J. 39:381-392. Ryu, C.-M., Farag, M. A., Hu, C.-H., Reddy, M. S., Paré, P. W., and Kloepper, J. W. 2004b. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol. 134:1017-1026. Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52:487-511.
摘要: 植物根圈促生細菌 (Plant growth-promoting rhizobacteria, PGPR) 可分泌植物生長的調控因子,如植物荷爾蒙,以活化根部的代謝作用,或是藉由分泌抗生物質、嵌鐵物質、競爭生態位與養份及誘導植株抗性來降低植物病原菌的侵害等方式來促進植物生長。本研究目的為施用蕈狀芽孢桿菌 Bacillus mycoides (BM) CHT2402 菌株於不同作物上觀察其促進作物生長的能力,且進一步針對萵苣萎凋病進行防治試驗並找出可能的防禦反應。本研究中發現 BM CHT2402具有產生吲哚乙酸 (Indole-3-Acetic Acid, IAA) 與氨 (NH3) 之特性,將 BM CHT2402的細菌懸浮液混拌入栽培介質後種植番茄、西瓜及萵苣植株三週後,具有促進植物生長的趨勢,其中以萵苣明豐三號品種與番茄農友 301 品種有顯著差異。進一步以澆灌法施用 BM CHT2402 細菌懸浮液於不同作物,結果並無促進植物生長的趨勢。將 BM CHT2402 以混拌法施用於三種品種的萵苣上,可促進萵苣植株的生長,但促進效果因不同品種而異。由萵苣萎凋病菌 (Fusarium oxysporum f. sp. lactucae) 所引起的萵苣萎凋病 (Fusarium wilt of lettuce) 為台灣葉萵苣夏季生產之主要限制因素,本研究中利用BM CHT2402 混拌栽培介質處理、或以水楊酸類似物--植物活化劑苯基 (1,2,3) 噻二唑-7-硫代羧酸硫甲酯 [benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, benzothiadiazole (BTH)] 及主成分為 harpin 蛋白的健旺肥料澆灌處理萵苣幼苗後,分析其對防治萵苣萎凋病的效果,結果顯示 BTH 澆灌處理與BM CHT2402 混拌栽培介質處理都有降低萵苣萎凋病罹病度的能力,而健旺澆灌處理則是各重複試驗的結果差異很大,以統計分析後,其效果並不顯著。BM CHT2402本身對於 F. oxysporum f. sp. lactucae FO-18 菌株沒有拮抗效果,以 BM CHT2402 混拌栽培介質處理後,萵苣植株之苯丙氨酸氨裂解酶 (phenylalanine ammonia lyase, PAL) 的基因表現量有降低的現象,而可溶性酚化物 (soluble phenolic compound) 的含量與 PAL 的酵素活性皆沒有提升,且會降低根部 guaiacol peroxidase (GPX) 的酵素活性,目前推測 BM CHT2402 施用後可搶先佔據根部生態位以降低萵苣萎凋病的罹病度或誘發植物體內其他的防禦反應。BTH 澆灌處理萵苣植株後,萵苣地上部與根部的 PAL 基因表現明顯增強,而萵苣根部中 PAL 與 GPX 的酵素活性都明顯高於對照組,此現象可能與 BTH 澆灌處理可以降低萵苣萎凋病的罹病度相關。不同重複之健旺處理對降低萵苣萎凋病罹病度的效果差異很大,應不適合用來防治萵苣萎凋病。
Plant growth-promoting rhizobacteria could enhance plant growth by providing plant growth regulators such as phytohormones to activate the metabolic activities of plant roots, so as to protect plants from attack by phytopathogens or reduce the disease severity by secreting antibiotics or siderphores, competing for the ecological niches or nutrition, and inducing plant defense responses. In this study, application of Bacillus mycoides (BM) CHT2402 was used to test its ability on growth promotion of different crops on controlling Fusarium wilt of lettuce and to find the possible defense responses.The abilities of BM CHT2402 to produce indole-3-acetic acid (IAA) and ammonia (NH3) were observed. Soil mixed with cell suspension of BM CHT2402 had the potential to promote the growth of tomato, watermelon, and lettuce plants, especially that Ming-feng NO. 3 cultivar of lettuce and KNOWN-YOU Farmers 301 cultivar of tomato showed significant difference. Soil irrigated with cell suspension of BM CHT2402, however, had no significant growth-promoting effects on these plants. Soil mixed with cell suspension of BM CHT2402 applied on three different cultivars of lettuce had different level of growth-promoting effects, and depending on the cultivars. Fusarium wilt caused by Fusarium oxysporum f. sp. lactucae (Fola) has become one of the most severe diseases of lettuce in Taiwan during summer. In this study, applications of BM CHT2402, SA analogue--benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, benzothiadiazole (BTH), and harpin containing Messenger STS (MSTS) on lettuce plants were tested on controlling this wilt disease. We found that both BTH irrigation and soil amendedt with BM CHT2402 could reduce disease severity of Fusarium wilt of lettuce, but the MSTS treatment was unable to control this disease. According to the results of duel culture, BM CHT2402 itself did not have antagonistic activity against Fola isolate FO-18. For soil amended with BM CHT2402, the gene expression of phenylalanine ammonia lyase (PAL) in lettuce was reduced, and the content of soluble phenolic compounds and PAL enzyme activity in lettuce plants did not increase, where as the enzyme activity of guaiacol peroxidase (GPX) in lettuce roots was reduced. The mechanism of BM CHT2402 on reducing disease severity of Fusarium wilt of lettuce was still not known, but it may be related to the ability of BM CHT2402 to colonize on lettuce roots, or to induce other defense response in lettuce. BTH treatment could induce the gene expression of PAL in lettuce plants, and increase the enzyme activity of PAL and GPX in lettuce roots. This may be the reason why BTH treatment could reduce disease severity of Fusarium wilt of lettuce. The potential of MSTS to reduce the disease severity caused by Fola was not significant, so MSTS treatment may not be suitable for controlling Fusarium wilt of lettuce.
URI: http://hdl.handle.net/11455/31234
其他識別: U0005-2008200814231800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200814231800


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