請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/31234
標題: Bacillus mycoides、水楊酸類似物及 harpin 蛋白對萵苣幼苗生長及抗病反應的效果
Effects of Bacillus mycoides, SA analogue, and harpin on the growth and disease-resistant response of lettuce seedlings
作者: 陳和緯
Chen, Her-Wei
關鍵字: Plant growth-promoting rhizobacteria
植物根圈促生細菌
Bacillus mycoides
BTH
Fusarium wilt of lettuce
Messenger STS
harpin
蕈狀芽孢桿菌
BTH
萵苣萎凋病
健旺
harpin
出版社: 植物病理學系所
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摘要: 植物根圈促生細菌 (Plant growth-promoting rhizobacteria, PGPR) 可分泌植物生長的調控因子,如植物荷爾蒙,以活化根部的代謝作用,或是藉由分泌抗生物質、嵌鐵物質、競爭生態位與養份及誘導植株抗性來降低植物病原菌的侵害等方式來促進植物生長。本研究目的為施用蕈狀芽孢桿菌 Bacillus mycoides (BM) CHT2402 菌株於不同作物上觀察其促進作物生長的能力,且進一步針對萵苣萎凋病進行防治試驗並找出可能的防禦反應。本研究中發現 BM CHT2402具有產生吲哚乙酸 (Indole-3-Acetic Acid, IAA) 與氨 (NH3) 之特性,將 BM CHT2402的細菌懸浮液混拌入栽培介質後種植番茄、西瓜及萵苣植株三週後,具有促進植物生長的趨勢,其中以萵苣明豐三號品種與番茄農友 301 品種有顯著差異。進一步以澆灌法施用 BM CHT2402 細菌懸浮液於不同作物,結果並無促進植物生長的趨勢。將 BM CHT2402 以混拌法施用於三種品種的萵苣上,可促進萵苣植株的生長,但促進效果因不同品種而異。由萵苣萎凋病菌 (Fusarium oxysporum f. sp. lactucae) 所引起的萵苣萎凋病 (Fusarium wilt of lettuce) 為台灣葉萵苣夏季生產之主要限制因素,本研究中利用BM CHT2402 混拌栽培介質處理、或以水楊酸類似物--植物活化劑苯基 (1,2,3) 噻二唑-7-硫代羧酸硫甲酯 [benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, benzothiadiazole (BTH)] 及主成分為 harpin 蛋白的健旺肥料澆灌處理萵苣幼苗後,分析其對防治萵苣萎凋病的效果,結果顯示 BTH 澆灌處理與BM CHT2402 混拌栽培介質處理都有降低萵苣萎凋病罹病度的能力,而健旺澆灌處理則是各重複試驗的結果差異很大,以統計分析後,其效果並不顯著。BM CHT2402本身對於 F. oxysporum f. sp. lactucae FO-18 菌株沒有拮抗效果,以 BM CHT2402 混拌栽培介質處理後,萵苣植株之苯丙氨酸氨裂解酶 (phenylalanine ammonia lyase, PAL) 的基因表現量有降低的現象,而可溶性酚化物 (soluble phenolic compound) 的含量與 PAL 的酵素活性皆沒有提升,且會降低根部 guaiacol peroxidase (GPX) 的酵素活性,目前推測 BM CHT2402 施用後可搶先佔據根部生態位以降低萵苣萎凋病的罹病度或誘發植物體內其他的防禦反應。BTH 澆灌處理萵苣植株後,萵苣地上部與根部的 PAL 基因表現明顯增強,而萵苣根部中 PAL 與 GPX 的酵素活性都明顯高於對照組,此現象可能與 BTH 澆灌處理可以降低萵苣萎凋病的罹病度相關。不同重複之健旺處理對降低萵苣萎凋病罹病度的效果差異很大,應不適合用來防治萵苣萎凋病。
Plant growth-promoting rhizobacteria could enhance plant growth by providing plant growth regulators such as phytohormones to activate the metabolic activities of plant roots, so as to protect plants from attack by phytopathogens or reduce the disease severity by secreting antibiotics or siderphores, competing for the ecological niches or nutrition, and inducing plant defense responses. In this study, application of Bacillus mycoides (BM) CHT2402 was used to test its ability on growth promotion of different crops on controlling Fusarium wilt of lettuce and to find the possible defense responses.The abilities of BM CHT2402 to produce indole-3-acetic acid (IAA) and ammonia (NH3) were observed. Soil mixed with cell suspension of BM CHT2402 had the potential to promote the growth of tomato, watermelon, and lettuce plants, especially that Ming-feng NO. 3 cultivar of lettuce and KNOWN-YOU Farmers 301 cultivar of tomato showed significant difference. Soil irrigated with cell suspension of BM CHT2402, however, had no significant growth-promoting effects on these plants. Soil mixed with cell suspension of BM CHT2402 applied on three different cultivars of lettuce had different level of growth-promoting effects, and depending on the cultivars. Fusarium wilt caused by Fusarium oxysporum f. sp. lactucae (Fola) has become one of the most severe diseases of lettuce in Taiwan during summer. In this study, applications of BM CHT2402, SA analogue--benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, benzothiadiazole (BTH), and harpin containing Messenger STS (MSTS) on lettuce plants were tested on controlling this wilt disease. We found that both BTH irrigation and soil amendedt with BM CHT2402 could reduce disease severity of Fusarium wilt of lettuce, but the MSTS treatment was unable to control this disease. According to the results of duel culture, BM CHT2402 itself did not have antagonistic activity against Fola isolate FO-18. For soil amended with BM CHT2402, the gene expression of phenylalanine ammonia lyase (PAL) in lettuce was reduced, and the content of soluble phenolic compounds and PAL enzyme activity in lettuce plants did not increase, where as the enzyme activity of guaiacol peroxidase (GPX) in lettuce roots was reduced. The mechanism of BM CHT2402 on reducing disease severity of Fusarium wilt of lettuce was still not known, but it may be related to the ability of BM CHT2402 to colonize on lettuce roots, or to induce other defense response in lettuce. BTH treatment could induce the gene expression of PAL in lettuce plants, and increase the enzyme activity of PAL and GPX in lettuce roots. This may be the reason why BTH treatment could reduce disease severity of Fusarium wilt of lettuce. The potential of MSTS to reduce the disease severity caused by Fola was not significant, so MSTS treatment may not be suitable for controlling Fusarium wilt of lettuce.
URI: http://hdl.handle.net/11455/31234
其他識別: U0005-2008200814231800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200814231800
顯示於類別:植物病理學系

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