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標題: Penicillium citrinum V3F-3和Aspergillus flavus V5F-13發酵液對芥菜黑斑病的防病效果及其抑制物質之特性
Control of black leaf spot of mustard cabbage by liquid cultures of Penicillium citrinum V3F-3 and Aspergillus flavus V5F-13, and characteristics of the inhibitory substances
作者: 陳妍廷
Chen, Yen-Ting
關鍵字: black leaf spot of mustard cabbage
Alternaria brassicicola
Penicillium citrinum
Aspergillus flavus
liquid culture
Alternaria brassicicola
Penicillium citrinum
Aspergillus flavus
出版社: 植物病理學系所
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摘要: 對永續農業而言,以其他微生物降低病原菌族群的方式來作為生物防治的策略是很重要的。在此研究篩選出能夠利用蔬菜組織來繁殖的土壤微生物,測試其蔬菜汁發酵液對Alternaria brassicicola引起的芥菜黑斑病的防治效果的試驗中,一共分離到69株放線菌、101株細菌和71株真菌,其中有1株細菌和5株真菌的發酵液,能夠減少罹病率及病斑大小。V3F-3與V5F-13菌株的發酵液能抑制A. brassicicola孢子發芽。利用型態與分生方法鑑定此二種真菌為Penicillium citrinum和Aspergillus flavus。P. citrinum V3F-3發酵液的冷凍乾燥粉末的methanol萃取液,以及A. flavus V5F-13發酵液的冷凍乾燥粉末的50% ethanol萃取液的抑制效果最佳。P. citrinum V3F-3發酵液中的抑制物質為殺菌作用,且能完全被活性炭所吸收;而A. flavus V5F-13發酵液中的抑菌物質為半殺菌作用,僅部分會被活性炭所吸收。二種抑制物質皆不帶電,且在酸性或鹼性環境下都非常穩定。P. citrinum V3F-3萃取液的抑制物質作用在pH 3-4時最佳,A. flavus V5F-13萃取液則是在pH 3-6時最佳。
Biocontrol of pathogens, the total or partial destruction of pathogen populations by other organisms, is important to the sustainable agriculture. Soil microorganisms capable of utilizing vegetable tissues for multiplication were isolated and their vegetable liquid cultures were tested for ability of control black leaf spot of mustard cabbage caused by Alternaria brassicicola. A total of 69 actinomycetes, 101 bacteria and 71 fungi were isolated and tested. Among them 1 bacterium and 5 fungi were able to reduce the disease incidence and lesion size of black leaf spot. The liquid cultures of V3F-3 and V5F-13 were inhibitory to conidial germination of A. brassicicola Aba-31. These 2 fungi were identified as Penicillium citrinum and Aspergillus flavus by morphological and molecular methods. Inhibitory substances in the freeze dried powder of liquid cultures of V3F-3 and V5F-13 were most efficiently extracted with methanol and 50% ethanol extract, respectively. The inhibitory substance in P. citrinum V3F-3 culture was fungicidal, and it was completely adsorbed by activated charcoal. The inhibitory substance in A. flavus V5F-13 culture was semi-fungicidal fungicidal, and it was partly adsorbed by activated charcoal. Both inhibitory substances did not have charges on their molecules, and were very stable under high or low pH value. The extracts of P. citrinum V3F-3 and A. flavus V5F-13 were most active at pH 3-4 and pH 3-6, respectively.
其他識別: U0005-2701201011534400
Appears in Collections:植物病理學系



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