Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31357
標題: Penicillium citrinum V3F-3和Aspergillus flavus V5F-13發酵液對芥菜黑斑病的防病效果及其抑制物質之特性
Control of black leaf spot of mustard cabbage by liquid cultures of Penicillium citrinum V3F-3 and Aspergillus flavus V5F-13, and characteristics of the inhibitory substances
作者: 陳妍廷
Chen, Yen-Ting
關鍵字: black leaf spot of mustard cabbage
芥菜黑斑病
Alternaria brassicicola
Penicillium citrinum
Aspergillus flavus
liquid culture
Alternaria brassicicola
Penicillium citrinum
Aspergillus flavus
發酵液
出版社: 植物病理學系所
引用: 李文權。1988。高等植物的化學防衛物質。科學農業 36:109-144。 林俊義、安寶貞、張清安、羅朝村、謝廷芳。2004。作物病害之非農藥防治 (再版)。行政院農業委員會農業試驗所編印。台中。53頁。 張清安、林瑩達。1992。應用無病毒苗防治百香果病毒病。病蟲害非農藥防治技術研討會專刊:349-359頁。中華植物保護學會出版。台中。 黃新川、李倩雲。1997。健康蕉苗之培育。健康清潔植物培育研習會研討會專刊:1-4頁。嘉義。 黃鴻章、黃振文、謝廷芳。2008。植物病害之生物防治。永續農業之植物病害管理:176-189頁。台中。 行政院農業委員會農業藥物毒物試驗所。2007。植物保護手冊。 蔡竹固、黃啟鐘。1994。十字花科蔬菜病蟲草害及其綜合防治。嘉義農專推廣簡訊第49期:11-19。 鄧汀欽、王怡玎。2003。台灣青蒜健康種蒜之培育與應用。植病會刊 12:1-9。 盧耀村、林俊義、許芳源。1992。馬鈴薯無病毒種原之培育與應用。病蟲害非農藥防治技術研討會專刊:325-347頁。中華植物保護協會出版。台中。 謝廷芳、黃晉興、謝麗娟、胡敏夫、柯文雄。2005。植物萃取液對植物病原真菌之抑制效果. 植病會刊 14:59-66。 Ansari, N. A., Kahn, M. W., and Muheet, A. 1990. Evaluation of some fungicides for seed treatment and foliar application in management of damping-off of seedlings and blight of rapeseed caused by Alternaria brassicae. Mycopathologia 110: 163-167. Brown, A. W. A. 1978. Ecology of Pesticides. John Wiley & Sons, New York. Chupp, C., and Sherf, A. F. 1960. Vegetable diseases and their control. The Ronald Press Company. New York. Cohen, R. A. 2002. β-aminobutyric acid-induced resistance against plant pathogens. Plant Dis. 86: 448-457. Curtis, R. F., Hassall, C. H., and Nazar, M. 1968. The biosynthesis of phenols. XV. Some metabolites of Penicillium citrinum related to citrinin. J. Chem. Soc. 1: 85-93. Cox, R. H., Hernandez, O., Dorner, J. W., Cole, R. J., and Fennell, D. I. 1979. A new isochroman mycotoxin isolated from Penicillium steckii. J. Agric. Food Chem. 27: 999-1001. Daayf, F., Schmitt, A., and Bélanger, R. R. 1995. The effects of plant extracts of Reynoutria sachalinensis on powdery mildew development and leaf physiology of long English cucumber. Plant Dis. 79: 577-580. Endo, A., Kuroda, M., and Tsujita, Y. 1976. ML-236 A, B, C, new inhibitors of cholesterogenesis produced by Penicillium citrinum. J. Antibiot. 29: 1346-1348. Farley, J. D., Lockwood, J. L. 1968. The suppression of actinomycetes by PCNB in culture media used for enumerating soil bacteria. Phytopathology 58: 714-715. Farr, D. F., Bills, G. F., Chamuris, G. P., and Rossman, A. Y. 1989. Fungi on Plant and Plant Products in the United States. American Phytopathological Society Press, St. Paul, MN. Fawcett, C. H. and Spencer, D. M. 1969. Natural antifungal compounds. Pages 637-669 in: Fungicides, an Advanced Treatise Vol. II. Chemistry and Physiology. D. C. Torgeson, ed. Academic Press, New York. Ho, W. C., Ko, W. H. 1980. A simple medium for selective isolation and enumeration of soil actinomycetes. Annals of the Phytopathological Society of Japan 46: 634-638. Ho, W. C., Su, H. J., Li, J. W., Ko, W. H. 2006. Effect of extracts of Chinese medicinal herbs on spore germination of Alternaria brassicicola, and nature of an inhibitory substance from gallnuts of Chinese sumac (Rhus chinensis). Can. J. Pathol. 28: 519-525. Ho, W. C., Wu, T. Y., Su, H. J., and Ko, W. H. 2007. Effect of oriental medicinal plant extracts on spore germination of Alternaria brassicicola and nature of inhibitory substances from speedweed. Plant Dis. 91: 1621-1624. Huang, H. C., and Acharya, S. N. 2003. Pages 153-163 in: Advances in plant disease management. Research Signpost. Canada. Huang, H. C., Marshall, H. H., Collicutt, L. M., McLaren, D. L., and Kokko, M. J. 1993. Screening hardy chrysanthemums for resistance to Fusarium oxysporum f. sp. chrysanthemi Race 2. Plant Pathol. Bull. 2: 103-105. Humpherson-Jones, F. M. 1989. Survival of Alternaria brassicae and Alternaria brassicicola on crop debris of oilseed rape and cabbage. Ann. Appl. Biol. 115: 45-50. Humpherson-Jones, F. M., and Phelps, K. 1989. Climatic factors influencing spore produciton in Alternaria brassicae and Alternaria brassicicola. Ann. Appl. Biol. 114: 449-458. Hwang, S. C., and Ko, W. H. 1988. Mutants of Cavendish banana resistant to race 4 of Fusarium oxysporum f. sp. cubense. Plant Prot. Bull. 30: 386-392. Intana, W., Suwanno, T., and Chamswarng, C. 2005. Use of antifungal metabolite from Trichoderma virens for controlling Chinese kale leaf spots caused by Alternaria brassicicola. Walailak J Sci & Tech 2: 1-9. Isman, B. M. 2000. Plant essential oils for pest and disease management. Crop Prot. 19: 603-608. Kishore, G. K., Pande, S., and Harish, S. 2007. Evaluation of essential oils and their components for broad-spectrum antifungal activity and control of late leaf spot and crown rot diseases in peanut. Plant Dis. 91: 375-379. Ko, W. H., Chang, H. S., and Su, H. J. 1978. Isolates of Phytophthora cinnamomi from Taiwan as evidence for an Asian origin of the species. Trans. Br. Mycol. Soc. 71: 496-499. Ko, W. H., L. L. Chase, and R. K. Kunimoto. 1973. A microsyringe method for determining concentration of fungal propagules. Phytopathology 63: 1206-1207. Ko, W. H., and Hora, F. K. 1971. A selective medium for the quantitative determination of Rhizoctonia solani in soil. Phytopathology 61: 707-710. Ko, W. H., Wang, S. Y., Hsieh, T. F., and Ann, P. J. 2003. Effects of sunflower oil on tomato powdery mildew caused by Oidium neolycopersici. J. Phytopathol. 151: 144-148. Kozlovskii, A. G., Stefanova-Avramova, L., Reshetilova, T. A., Sakharovskii, V. G., and Adanin, V. M. 1981. Clavine ergoalkaloids-metabolites of Penicillium gorlenkoanum. Prikl. Biokhim. Mikrobiol. 17: 806-812. Leifert, C., Li, H., Chidburee, S., Hampson, S., Workman, S., Sigee, D., Epton, H. A. S., and Harbour, A. 1995. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J. Appl. Bacteriol. 78: 97-108. Leifert, C., Sigee, D. C., Stanley, R., Knight, C., and Epton, H. A. S. 1993. Biocontrol of Botrytis cinerea and Alternaria brassicicola on Dutch white cabbage by bacterial antagonists at cold-store tempertures. Plant Pathol. 42: 270-279. Martin, H., and Salmon, E. S. 1930. Vegetable oils as fungicides. Nature 126: 58. Maude, R. B., and Humpherson-Jones, F. M. 1980a. Studies on the seed-borne phases of dark leaf spot (Alternaria brassicicola) and grey leaf spot (Alternaria brassicae) of brassicas. Ann. Appl. Biol. 95: 331-319. Maude, R. B., and Humpherson-Jones, F. M. 1980b. The effect of iprodione on the seed-borne phase of Alternaria brassicicola. Ann. Appl. Biol. 95: 321-327. Maude, R. B., Humpherson-Jones, F. M., and Shuring, C.G. 1984. Treatments to control Phoma and Alternaria infections of brassica seeds. Plant Pathol. 33: 525-535. Muto, M., Huang, J. W., and Takahashi, H. 2004. Effect of water-soluble extracts of radish seed meal on control of lettuce leaf spot (Acremonium lactucae Lin et al.). Plant Pathol. Bull. 13: 275-282. Muto, M., Mulabagal, V., Huang, H. C., Takahashi, H., Tsay, H. S., and Huang, J. W. 2006. Toxicity of black nightshade (Solanum nigrum) extracts on Alternaria brassicicola, causal agent of black leaf spot of Chinese cabbage (Brassica pekinensis). J. Phytopathol. 154: 45-50. Muto, M., Takahshi, H., Ishihara, K., Yuasa, H., and Huang, J. W. 2005. Control of black leaf spot (Alternaria brassicicola) of crucifers by extracts of black night shade (Solanum nigrum). Plant Pathol. Bull. 14: 25-34. Muto, M., Takahshi, H., Ishihara, K., Yuasa, H., and Huang, J. W. 2005. Antimicrobial activity of medicinal plants used by indigenous people in Taiwan. Plant Pathol. Bull. 14: 13-24. Olutiola, P. O., and Akintunde, O. A. 1979. Pectin lyase and pectin methylesterase production by Penicillium citrinum. Trans. Br. Mycol. Soc. 72: 49-55. Ozgonen, H., and Erkilic, A. 2007. Growth enhancement and Phytophthora blight(Phytophthora capsici Leonian) control by arbuscular mycorrhizal fungal inoculation in pepper. Crop Protect. 26: 1682-1688. Poala, P., Wilcox, C., and Finland, M. 1970. Effect of pH of medium and size of inoculum on activity of antibiotics against group D Streptococcus (Enterococcus). Appl. Microbiol. 19: 623-637. Powell, K. A., Renwick, A., and Peberdy, J. F. 1994. The Genus Aspergillus: From Taxonomy and Genetics to Industrial Application. Plenum Press. New York and London. Price, M. J., and Worth, G. K. 1974. The occurrence of ergosta-4, 6, 8(14), 22-tetraen-3-one in several fungi. Aust. J. Chem. 27: 2505-2507. Rangel, J. F. 1945. Two Alternaria diseases of cruciferous plants. Phytopathology 35: 1002-1007. Scott, P. M. 1977. Penicillium mycotoxin. Pages 283-356 in: Mycotoxic Fungi, Mycotoxins, Mycotoxicoses, Vol. I. T. D. Wyllie and L. G. Morehouse, eds. Marcel Dekker, Inc. New York. Sharma, A. K., J. S. Gupta, and R. K. Maheshwari. 1984. The relationship of Streptomyces arabicus to Alternaria brassicae (Berk.) Sacc. and Alternaria brassicicola (Schew.) Wiltshire on the leaf surface of yellow sarson and taramira. Geobios New Rep. 3: 83-84. Sharma, A. K., J. S. Gupta, and S. P. Singh. 1985. Effect of temperature on the antifungal activity of Streptomyces arabicus against Alternaria brassicae (Berk) Sacc. and A. brassicicola (Schew.) Wiltshire. Geobios New Rep. 12: 168-169. Sharma, O. P., and Sharma, K. D. 1980. Studies on in vitro production of proteolytic enzymes by some leather deteriorating fungi. Rev. Roum. Biochim. 17: 209-215. Singh, B. S., and Saksena, S. B. 1981. Estimation of extracellular Cl and Cx cellulases produced by certain species of Penicillium. Natl. Acad. Sci. Lett. 4: 431-432. Sun, S. K., and Huang, J. W. 1985. Formulated soil amendment for controlling Fusarium wilt and other soilborne diseases. Plant Dis. 69: 917-920. Tony Lam, Y. K., Gullo, U. P., Goegelman, R. T., Jorn, D., Huang, L., De Riso, C., Monaghan, R. L., and Putter, I. 1981. Dihydrocompactin, a new potent inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Penicillium citrinum. J. Antibiot. 34: 614-616. Turner, W. B., and Aldridge, D. C. 1983. Fungal Metabolites II. Academic Press. New York. Tzean, S. S., Chiu, S. C., Chen, J. L., Hseu, S. H., Lin, G. H., Liou, G. Y., Chen, C. C., and Hsu, W. H. 1990. Aspergillus and related teleomorphs from Taiwan. Mycol. Monograph No. 1. FIRDI. Hsinchu. Taiwan. R.O.C. Tzean, S. S., Chiu, S. C., Chen, J. L., Hseu, S. H., Lin, G. H., Liou, G. Y., Chen, C. C., and Hsu, W. H. 1994. Penicillium and related teleomorphs from Taiwan. Mycol. Monograph No. 9. FIRDI. Hsinchu. Taiwan. R.O.C. Valkonen, J. P. T., and Koponen, H. 1990. The seed-borne fungi of Chinese cabbage (Brassica pekinensis), their pathogenicity and control. Plant Pathol. 39: 510-516. Vesonder, R. F., Ciegler, A., Fennell, D., Tjarks, L. W., and Jensen, A. H. 1976. Curvularin from Penicillium baradicum Baghdadi NRRL 3754, and biological effects. J. Environ. Sci. Health B11: 289-297. Verma, P. R., and Saharan, G. S. 1994. Monograph of Alternaria diseases of crucifers. Saskatoon Research Centre Technical Bulletin 1994–6E. Agriculture and Agri-food Canada. Saskatoon. Vilgalys, R., and Hester, M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172: 4238–4246. Vining, L. C., McInnes, A. G., Smith, D. G., Wright, J. L. C., and Taber, W. A. 1982. Dimeric clavinet alkaloids produced by Penicillium citreo-viride. FEMS Symp. 13: 243-251. White, T. J., Bruns, T., Lee, S., Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols, a Guide to Methods and Applications. M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White eds. Academic Press. San Diego. CA. 482pp. Xu, X. L., Ko, W. H. 1998. A quantitative confined inoculation method for studies of pathologenicity of fungi on plants. Bot. Bull. Acad. Sin. 39: 187-190. Yeh, S. D., and Gonsalves, D. 1984. Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74: 1086-1091.
摘要: 對永續農業而言,以其他微生物降低病原菌族群的方式來作為生物防治的策略是很重要的。在此研究篩選出能夠利用蔬菜組織來繁殖的土壤微生物,測試其蔬菜汁發酵液對Alternaria brassicicola引起的芥菜黑斑病的防治效果的試驗中,一共分離到69株放線菌、101株細菌和71株真菌,其中有1株細菌和5株真菌的發酵液,能夠減少罹病率及病斑大小。V3F-3與V5F-13菌株的發酵液能抑制A. brassicicola孢子發芽。利用型態與分生方法鑑定此二種真菌為Penicillium citrinum和Aspergillus flavus。P. citrinum V3F-3發酵液的冷凍乾燥粉末的methanol萃取液,以及A. flavus V5F-13發酵液的冷凍乾燥粉末的50% ethanol萃取液的抑制效果最佳。P. citrinum V3F-3發酵液中的抑制物質為殺菌作用,且能完全被活性炭所吸收;而A. flavus V5F-13發酵液中的抑菌物質為半殺菌作用,僅部分會被活性炭所吸收。二種抑制物質皆不帶電,且在酸性或鹼性環境下都非常穩定。P. citrinum V3F-3萃取液的抑制物質作用在pH 3-4時最佳,A. flavus V5F-13萃取液則是在pH 3-6時最佳。
Biocontrol of pathogens, the total or partial destruction of pathogen populations by other organisms, is important to the sustainable agriculture. Soil microorganisms capable of utilizing vegetable tissues for multiplication were isolated and their vegetable liquid cultures were tested for ability of control black leaf spot of mustard cabbage caused by Alternaria brassicicola. A total of 69 actinomycetes, 101 bacteria and 71 fungi were isolated and tested. Among them 1 bacterium and 5 fungi were able to reduce the disease incidence and lesion size of black leaf spot. The liquid cultures of V3F-3 and V5F-13 were inhibitory to conidial germination of A. brassicicola Aba-31. These 2 fungi were identified as Penicillium citrinum and Aspergillus flavus by morphological and molecular methods. Inhibitory substances in the freeze dried powder of liquid cultures of V3F-3 and V5F-13 were most efficiently extracted with methanol and 50% ethanol extract, respectively. The inhibitory substance in P. citrinum V3F-3 culture was fungicidal, and it was completely adsorbed by activated charcoal. The inhibitory substance in A. flavus V5F-13 culture was semi-fungicidal fungicidal, and it was partly adsorbed by activated charcoal. Both inhibitory substances did not have charges on their molecules, and were very stable under high or low pH value. The extracts of P. citrinum V3F-3 and A. flavus V5F-13 were most active at pH 3-4 and pH 3-6, respectively.
URI: http://hdl.handle.net/11455/31357
其他識別: U0005-2701201011534400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2701201011534400
Appears in Collections:植物病理學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.