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標題: 抗台灣番茄捲葉病毒與番茄斑萎病毒無篩選標誌轉基因植物之研發
Development of marker-free transgenic plants with resistance to Tomato leaf curl Taiwan virus and Tomato spotted wilt virus
作者: 林靜宜
Lin, Ching-Yi
關鍵字: 無篩選標誌基因
出版社: 植物病理學系所
引用: 參考文獻 Abhary MK, Anfoka GH, Nakhla MK, Maxwell DP (2006) Post-transcriptional gene silencing in controlling viruses of the Tomato yellow leaf curl virus complex. Arch Virol 151:2349-2363. Akad F, Eybishtz A, Edelbaum D, Gorovits R, Dar-Issa O, Iraki N, Czosnek H (2007) Making a friend from a foe: expressing a GroEL gene from the whitefly Bemisia tabaci in the phloem of tomato plants confers resistance to tomato yellow leaf curl virus. Arch Virol 152:1323-1339. Antignus Y, Vunsh R, Lachman O, Pearlsman M, Maslenin L, Hananya U, Rosner A (2004) Truncated Rep gene originated from Tomato yellow leaf curl virus-Israel [Mild] confers strain-specific resistance in transgenic tomato. Ann Appl Biol 144: 39-44. Araga˜o FJL, Ribeiro SG, Barros LMG, Brasileiro ACM, Maxwell DP, Rech EL, Faria JC (1998) Transgenic beans (Phaseolus vulgaris L.) engineered to express viral antisense RNAs show delayed and attenuated symptoms to bean golden mosaic geminivirus. Mol Breed 4:491-499. Asad S, Haris WA, Bashir A, Zafar Y, Malik KA, Malik NN, Lichtenstein CP (2003) Transgenic tobacco expressing geminiviral RNAs is resistant to the serious viral pathogen causing cotton leaf curl disease. Arch Virol 148:2341-2352. Azzam O, Diaz O, Beaver JS, Gilbertson RL, Russell DR, Maxwell DP (1996) Transgenic beans with the bean golden mosaic geminivirus coat protein gene are susceptible to virus infection. Ann Rep Bean Improv Coop 39:276-277. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281-297. Baulcombe DC (2004) RNA silencing in plants. Nature 431:356–363. Baulcombe DC, English JJ (1996) Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants. Curr Opin Biotechnol 7:173-180. Behjatnia SA A, Dry IB, Rezaian MA (1998) Identification of the replication-associated protein binding domain within the intergenic region of tomato leaf curl geminivirus. Nucleic Acids Res 26:925-931. Bejarano ER, Lichtenstein CP (1994) Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses. Plant Mol Biol 24:241-248. Bendahmane M, Gronenborn B (1997) Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA. Plant Mol Biol 33:351-357. Bennett PM, Livesey CT, Nathwani D, Reeves DS, Saunder JR, Wise R (2004) An assessment of the risks associated with the use of antibiotic resistance genes in genetically modified plants: report of the Working Party of the British Society for Antimicrobial Chemotherapy. J. Antimicrob Chemother 53:418-431. Bonfim K, Faria JC, Nogueira EO, Mendes EA, Aragão FJ (2007) RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris). Mol Plant Microbe Interact 20:717-726. Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234-243. Briddon RW, Stanley J (2006) Subviral agents associated with plant single-stranded DNA viruses. Virology 344:198-210. Briddon RW, Watts J, Markham PG, Stanley J (1989) The coat protein of beet curly top virus is essential for infectivity. Virology 172:628-633. Brunetti A, Tavazza R, Noris E, Lucioli A, Accotto GP, Tavazza M (2001) Transgenically expressed T-Rep of tomato yellow leaf curl Sardinia virus acts as a trans-dominant-negative mutant, inhibiting viral transcription and replication. J Virol 75:10573-10581. Brunetti A, Tavazza M, Noris E, Tavazza R, Caciagli P, Ancora G, Crespi S, Accotto GP (1997) High expression of truncated viral Rep protein confers resistance to Tomato yellow leaf curl virus in transgenic tomato plants. Mol Plant-Microbe Interact 10:571-579. Bucher E, Lohuis D, Pieter M, van Poppel JA, Geerts-Dimitriadou C, Goldbach R, Prins M (2006) Multiple virus resistance at a high frequency using a single transgene construct. J Gen Virol 87:3697-3701. Bucher E, Sijen T, de Haan P, Goldbach R, Prins M (2003) Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. J Virol 77:1329-1336. Carr JP, Marsh LE, Lomonossoff GP, Sekiya ME, Zaitlin M (1992) Resistance to tobacco mosaic virus induced by the 54-kDa gene sequence requires expression of the 54-kDa protein. Mol Plant-Microbe Interact 5:397-404. Charng YC, Li KT, Tai HK, Lin NS, Tu J (2008) An inducible transposon system to terminate the function of a selectable marker in transgenic plants. Mol Breed 21:359-368. Chatterji A, Beachy RN, Fauquet CM (2001) Expression of the oligomerization domain of the replication-associated protein (Rep) of tomato leaf curl New Delhi virus interferes with DNA accumulation of heterologous geminiviruses. J Biol Chem 276:25631-25638. Chellappan P, Masona MV, Vanitharani R, Taylor NJ, Fauquet CM (2004a) Broad spectrum resistance to ssDNA viruses associated with transgene-induced gene silencing in cassava. Plant Mol Biol 56:601-611. Chellappan P, Vanitharani R, Fauquet CM (2004b) Short interfering RNA accumulation correlates with host recovery in DNA virus-infected hosts, and gene silencing targets specific viral sequences. J Virol 78:7465-7477. Chellappan P, Vanitharani R, Fauquet CM (2005) MicroRNAbinding viral protein interferes with Arabidopsis development. Proc Natl Acad Sci USA 102:10 381-10 386. Chen S, Li X, Liu X, Xu H, Meng K, Xiao G, Wei X, Wang F, Zhu Z (2005) Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNA binary vector system. Plant Cell Rep 23:625-31. Cooper B, Lapidot M, Heick JA, Dodds JA, Beachy RN (1995) A defective movement protein of TMV in transgenic plants confer resistance to multiple viruses whereas the functional analog increases susceptibility. Virology 206:307-313. Cui X, Li G, Wang D, Hu D, Zhou X (2005) A begomovirus DNAβ-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus. J Virol 79:10764-10775. Cui X, Tao X, Xie Y, Fauquet CM, Zhou X (2004) A DNAβ associated with Tomato yellow leaf curl China virus is required for symptom induction. J Virol 78:13966-13974. Dale PJ, Clarke B, Fontes EMG (2002) Potential for the environmental impact of transgenic crops. Nat Biothchnol 20:567-574. Daley M, Knauf VC, Summerfelt KR, Turner JC (1998) Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17:489-496. Dalmay T, Hamilton AJ, Rudd S, Angell S, Baulcombe DC (2000) An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 101:543-553. Darbani B, Eimanifar A, Stewart CN, Camargo WN (2007) Methods to produce marker-free transgenic plants. Biotechnol J 2:83-90. Day AG, Bejarano ER, Buck KW, Burrell M, Lichtenstein CP (1991) Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus. Proc Natl Acad Sci USA 88:6721-6725. de Block M, Debrouwer D (1991) Two T-DNA’s co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theor Appl Genet 82:257-263. de Framond AJ, Back EW, Chilton WS, Kayes L, Chilton MD (1986) Two unlinked T-DNAs can transform the same tobacco plant cell and segregate in the F1 generation. Mol Gen Genet 202:125-131. de Haan P, Kormelink R, de Oliveira Resende R, Van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 71:2207-2216. de Haan P, Wagemakers L, Peters D, Goldbach RW (1990) The S RNA segment of Tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001-1007. de Neve M, de Buck S, Jacobs A, van Montagu M, Depicker A (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs. Plant J 11:15-29. Depicker A, Herman L, Jacobs A, Schell J, van Montagu M (1985) Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interaction. Mol Gen Genet 201:477-484. Desbize C, David C, Mettouchi A, Laufs J, Gronenborn R (1995) Rep protein of Tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication. Proc Natl Sci USA 92:5640-5644. Dong X, van Wezel R, Stanley J, Hong Y (2003) Functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing. J Virol 77:7026-7033. Dougherty WG, Lindbo JA, Smith HA, Parks TD, Swaney S, Proebsting WM (1994) RNA-mediated virus resistance in transgenic plants: Exploitation of a cellular pathway possibly involved in RNA degradation. Mol Plant-Microbe Interact 7:544-552. Dröge M, Pühler A, Selbitschka W (1998) Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. J Biotechnol 64:75-90. Duan YP, Powell CA, Webb SE, Purcifull DE, Hiebert E (1997) Geminivirus resistance in transgenic tobacco expressing mutated BC1 protein. Mol Plant-Microb Interact 10:617-623. Ebinuma H, Sugita K, Matsunaga E, Endo S, Yamada K, Komamine A (2001) Systems for the removal of a selection marker and their combination with a positive marker. Plant Cell Rep 20:383-392. FAO/WHO (2000) Safety aspects of genetically modified foods of plant origin. Report of a Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology. Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783-821. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, New York. Fermin G, Gonsalves D (2001) Towards the development of short synthetic genes for multiple virus resistance. Phytopathology 91:S6. Fondong VN, Reddy RV Chowda, Lu C, Hankoua B, Felton C (2007) The consensus N-myristoylation motif of a geminivirus AC4 protein is required for membrane binding and pathogenicity. Mol Plant-Microbe Interact 20:380-391. Fontes EPB, Eagle PA, Sipe PS, Luckow VA, Hanley-Bowdoin L (1994) Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. J Biol Chem 269:8459-8465. Freitas-Astua J, Purcifull DE, Polston JE, Hiebert E (2002) Tranditional and transgenic strategies for controlling tomato-infecting begomovirus. Fitopatol Bras 27:437-449. Frischmuth T, Engel M, Jeske H (1997) Beet curly top virus DI DNA-mediated resistance is linked to its size. Mol Breed 3:213-217. Frischmuth S, Stanley J (1998) Recombination between viral DNA and the transgenic coat protein gene of African cassava mosaic geminivirus. J Gen Virol 79:1265-1271. Fuentes A, Ramos PL, Fiallo E, Callard D, Sánchez Y, Peral R, Rodríguez R, Pujol M (2006) Intron-hairpin RNA derived from replication associated protein C1 gene confers immunity to tomato yellow leaf curl virus infection in transgenic tomato plants. Transgenic Res 15:291-304. Ghanim M, Morin S, Zeidan M, Czosnek H (1998) Evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly Bemisia tabaci. Virology 240:295-303. Gharsallah-Chouchane S, Gorsane F, Nakhla MK, Salus M, Martin CT, Maxwell DP, Marrakchi M, Fakhfakh H (2008) Evaluation of two gene-silencing constructs for resistance to tomato yellow leaf curl viruses in Nicotiana benthamiana plants. Acta Virologica 52:143-149. Gilreath P, Shuler K, Polston JE, Sherwood TA, McAvoy G, Stansly PA, Waldo E (2001) Tomato yellow leaf curl virus resistant tomato variety trials. Proc Fla State Hortic Soc 114:190-193. Gopal P, Kumar PP, Sinilal B, Jose J, Yadunandam A, Usha R (2007) Differential roles of C4 and bC1 in mediating suppression of post-transcriptional gene silencing: evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus. Virus Res 123:9-18. Grant SR (1999) Dissecting the mechanisms of posttranscriptional gene silencing: divide and conquer. Cell 96:303-306. Grumet R (1995) Genetic engineering for crop virus resistance. Hortscience 30: 449-456. Guo W, Jiang T, Zhang X, Li G, Zhou X (2008) Molecular variation of satellite DNA beta molecules associated with Malvastrum yellow vein virus and their role in pathogenicity. Appl Environ Microbiol 74:1909-1913. Hanley-Bowdoin L, Settlage SB, Orozco BM, Nagar S, Robertson D (1999) Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Plant Sci 18:71-106. Hanley-Bowdoin L, Settlage SB, Robertson D (2004) Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication. Mol Plant Pathol 5:149-156. Hao L, Wang H, Sunter G, Bisaro DM (2003) Geminivirus AL2 and L2 proteins interact with and inactivate SNF1 kinase. Plant Cell 15:1034-1048. Hare PD, Chua NH (2002) Excision of selectable marker genes from transgenic plants. Nat Biotechnol 20:575-580. Heritage J (2004) The fate of transgenes in the human gut. Nat Biotechnol 22:170-172. Hilje L, Costa HS, Stansly PA (2001) Cultural practices for managing Bemisia tabaci and associated viral diseases. Crop Protection 20:801-812. Hofer P, Bedford ID, Markham PG, Jeske H, Frischmuth T (1997) Coat protein gene replacement results in whitefly transmission of an insect nontransmissible geminivirus isolate. Virology 236:288-295. Hong Y, Stanley J (1995) Regulation of African cassava mosaic virus complementary-sense gene expression by N-terminal sequences of the replication-associated protein AC1. J Gen Virol 76:2415-2422. Hong Y, Stanley J (1996) Virus resistance in Nicotiana benthamiana conferred by African cassava mosaic virus replication-associated protein (AC1) transgene. Mol Plant–Microbe Interact 9:219-225. Hong Y, Saunders K, Hartley MR, Stanley J (1996) Resistance to geminivirus infection by virus-induced expression of dianthin in transgenic plants. Virology 220:119-127. Horsch RB, Fry JE, Hoffmann HL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229-1230. Hou YM, Sanders R, Ursin VM, Gilbertson RL (2000) Transgenic plants expressing geminivirus movement proteins: abnormal phenotypes and delayed infection by Tomato mottle virus in transgenic tomatoes expressing the Bean dwarf mosaic virus BV1 or BC1 proteins. Mol Plant-Microbe Interact 13:297-308. Ingham DJ, Pascal E, Lazarowitz SG (1995) Both bipartite geminivirus movement proteins define viral host range, but only BL1 determines viral pathogenicity. Virology 207:191-204. Jacob SS, Veluthambi K (2002) Generation of selection marker-free transgenic plants by cotransformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain. Plant Sci 163:801-806. Jan F-J, Fagoaga C, Pang S-Z, Gonsalves D (2000a) A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. J Gen Virol 81:235-242. Jan F-J, Fagoaga F, Pang S-Z, Gonsalves D (2000b) A single chimeric transgene derived from two distinct viruses confers multi-virus resistance in transgenic plants through homology-dependent gene silencing. J Gen Virol 81:2103-2109. Jaiwal PK, Sahoo L, Singh ND, Singh RP (2002) Strategies to deal with the concern about marker genes in transgenic plants: Some environment-friendly approaches. Curr Sci 83:128-136. Jose J, Usha R (2003) Bhendi yellow vein mosaic disease in India is caused by association of a DNA- satellite with a Begomovirus. Virology 305:310-317. Kikkert M, Verschoor AD, Kormelink R, Peters D, Goldbach R (2001) Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells. J Virol 75:1004-1012. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plant mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10:165-174. Kon T, Hidayat SH, Hase S, Takahashi H, Ikegami M (2006) The natural occurrence of two distinct begomoviruses associated with DNAb and a recombinant DNA in a tomato plant from Indonesia. Phytopathology 96:517-525. Kon T, Sharma P, Ikegami M (2007) Suppressor of RNA silencing encoded by the monopartite tomato leaf curl Java begomovirus. Arch Virol 152:1273-1282. Kormelink R, Storms M, Van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NSm protein of Tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56-65. Krake LR, Rezaian AM, Dry IB (1998) Expression of tomato leaf curl C4 gene produces virus-like symptoms in transgenic plants. Mol Plant-Microbe Interact 11:413-417. Kunik T, Salomon R, Zamir D, Navot N, Zeidan M, Michelson I, Gafni Y, Czosnek H (1994) Transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus. Biotechnology 12:500-504. Lapidot M, Friedman M (2002) Breeding for resistance to whitefly-transmitted geminiviruses. Ann Appl Biol 140:109-127. Lapidot M, Friedmann M, Pilowsky M, Ben-Joseph R, Cohen S (2001) The effect of host resistance on Tomato yellow leaf curl virus (TYLCV) on virus acquisition and transmission by its whitefly vector. Phytopathology 91:1209 -1213. Laufs J, Traut W, Heyraud F, Matzeit V, Rogers S, Schell J, Gronenborn B (1995) In vitro cleavage and joining at the viral origin of replication by the replicaion initiator protein of tomato yellow leaf curl virus. Proc Natl Acad Sci USA 92:3879-3883. Lewandowski DJ, Adkins S (2005) The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids. Virology 342:26-37. Lin CY, Fan FJ (2005) Current development of the strategies for generating marker-free transgenic plants. Plant Pathol Bull 14:159-176. Lippman Z, Martienssen R (2004) The role of RNA interference in heterochromatic silencing. Nature 431:364-370. Liu SJ, Bedford ID, Briddon RW, Markham PG (1997) Efficient whitefly transmission of African cassava mosaic geminivirus requires sequences from both genomic components. J Gen Virol 78:1791-1794. Lu H-J, Zhou CR, Gong Z-X, Upadhyaya NM (2001) Generation of selectable marker-free transgenic rice using double right border (DRB) binary vectors. Aust J Plant Physiol 28:241-248. Lucioli A, Noris E, Brunetti A, Tavazza R, Ruzza V, Castillo AG, Bejarano ER, Accotto GP, Tavazza M (2003) Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. J Virol 77:6785-6798. Matsuda N, Sharma P, Bajet NB, Ikegami M (2008) Molecular characterization of a new strain of tomato leaf curl Philippines virus and its associated satellite DNAbeta molecule: further evidence for natural recombination amongst begomoviruses. Arch Virol 153:961-967. McKnight TD, Lillis MT, Simpson RB (1987) Segregation of genes transferred to one plant cell from two separate Agrobacterium tumefaciens strains. Plant Mol Biol 8:439-445. Miki B, McHugh S (2004) Selection marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 170:193-232. Morin S, Ghanim M, Sobol I, Czosnek H (2000) The GroEL protein of the whitefly Bemisia tabaci interacts with the coat protein of transmissible and nontransmissible begomoviruses in the yeast two-hybrid system. Virology 276:404-416. Mubin M, Mansoor S, Hussain M, Zafar Y (2007) Silencing of the AV2 gene by antisense RNA protects transgenic plants against a bipartite begomovirus. Virol J 4:10. Neilsen KM, Bones AM, Smalla K, van Elsas JD (1998) Horizontal gene transfer from transgenic plants to terrestrial bacteria—a rare event? FEMS Microbiol Rev 22:79-103. Netherwood T, Martín-Orúe SM, O’Donnell AG, Gockling S, Graham J, Mathers JC, Gilbert HJ (2004) Assessing the survival of transgenic plant DNA in the human gastrointestinal tract. Nat Biotechnol 22:204-209. Noris E, Accotto GP, Tavazza R, Brunetti A, Crespi S, Tavazza M (1996) Resistance to tomato yellow leaf curl geminivirus in Nicotiana benthamiana plants transformed with a truncated viral C1 gene. Virology 224:130-138. Noris E, Vaira AM, Caciagli P, Masenga V, Gronenborn B, Accotto GP (1998) Amino acids in the capsid protein of tomato yellow leaf curl virus that are crucial for systemic infection, particle formation, and insect transmission. J Virol 72:10050-10057. Padidam M, Beachy RN, Fauquet CM (1996) The role of AV2 (“precoat”) and coat protein in viral replication and movement in tomato leaf curl geminivirus. Virology 224:390-404. Pang S-Z, Jan F-J, Gonsalves D (1997) Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants. Proc Natl Acad Sci USA 94:8261-8266. Pascal E, Goodlove PE, Wu LC, Lazarowitz SG (1993) Transgenic tobacco planta expressing the geminivirus BL1 protein exhibite symptoms of viral disease. Plant Cell 5:795-807. Pooggin M, Shivaprasad PV, Veluthambi K, Hohn T (2003) RNAi targeting of DNA virus in plants. Nat Biotechnol 21:131-132. Pooma W, Gillette WK, Jeffrey JL, Petty IT (1996) Host and viral factors determine the dispensability of coat protein for bipartite geminivirus systemic movement. Virology 218:264-268. Powell-Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738-743. Praveen S, Kushwaha CM, Mishra AK, Singh V, Jain RK, Varma A (2005a) Engineering tomato for resistance to tomato leaf curl disease using viral rep gene sequences. Plant Cell Tissue Organ Cult 83:311-318. Praveen S, Mishra AK, Antony G (2006) Viral suppression in transgenic plants expression chimeric transgene from tomato leaf curl virus and cucumber mosaic virus. Plant Cell Tissue Organ Cult 84:47-53. Praveen S, Mishra AK, Dasgupta A (2005b) Antisense suppression of replicase gene expression recovers tomato plants from leaf curl infection. Plant Sci 168:1011-1014. Prins M, Goldbach R (1998) The emerging problem of tospovirus infection and nonconventional methods of control. Trends Microbiol 6:31-35. Prins M, Kikkert M, Ismayadi C, de Graauw W, de Haan P, Goldbach R (1997) Charaterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing NSm gene sequence. Plant Mol Biol 33:235-243. Prins M, Resende Rde O, Anker C, Van Schepen A, De Haan P, Goldbach R (1996) Engineered RNA-mediated resistance to tomato spotted wilt virus is sequence specific. Mol Plant-Microbe Interact 9:416-418. Puchta H (2003) Marker-free transgenic plants. Plant Cell Tiss Org 74:123-134. Qu J, Ye J, Fang R (2007) Artificial MicroRNA-mediated virus resistance in plants. J Virol 81:6690-6699. Ramesh SV, Mishra AK, Praveen S (2007) Hairpin RNA-mediated strategies for silencing of tomato leaf curl virus AC1 and AC4 genes for effective resistance in plants. Oligonucleotides 17:251-257. Rigden JE, Krake LR, Rezaian MA, Dry IB (1994) ORF C4 of Tomato Leaf Curl Geminivirus Is a Determinant of Symptom Severity. Virology 204:847-850. Rodriguez-Negrete EA, Carrillo-Tripp J, Rivera-Bustamante RF (2009) RNA silencing against geminivirus: complementary action of posttranscriptional gene silencing and transcriptional gene silencing in host recovery. J Virol 83:1332-1340. Rojas MR, Jiang H, Salati R, Xoconostle-Cázares B, Sudarshana MR, Lucas WJ, Gilbertson RL (2001) Functional analysis of proteins involved in movement of the monopartite begomovirus, Tomato yellow leaf curl virus. Virology 291:110-125. Rommens CM (2004) All–native DNA transformation: a new approach to plant genetic engineering. Trends Plant Sci 9:457-464. Rommens CM, Humara JM, Ye J, Yan H, Richael C, Zhang L, Perry R, Swords K (2004) Crop improvement through modification of the plant’s own genome. Plant Physiol 135:421-431. Safarnejad MR, Fischer R, Commandeur U (2009) Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamiana. Arch Virol 154:457-467. Sanderfoot AA, Ingham DJ, Lazarowitz SG (1996) A viral movement protein as a nuclear shuttle. Plant Physiol 110:23-33. Sanford JC, Johnston SA (1985) The concept of parasite-derived resistance-deriving resistance genes from the parasite''s own genome. J Theor Biol 113:395-405. Sangare A, Deng D, Fauquet CM, Beachy RN (1999) Resistance to African cassava mosaic virus conferred by mutant of the putative NTP-binding domain of the Rep gene (AC1) in Nicotiana benthamiana. Mol Breed 5:95-102. Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J (2000) A unique virus complex causes ageratum yellow vein disease. Proc Natl Acad Sci USA 97:6890-6895. Seal SE, van den Bosch F, Jeger MJ (2006) Factors influencing begomovirus evolution and their increasing global significance: implications for sustainable control. Crit Rev Plant Sci 25:23-46. Seemanpillai M, Dry I, Randles J, Rezaian A (2003) Transcriptional silencing of geminiviral promoter-driven transgenes following homologous virus infection. Mol Plant-Microb Interact 16:429-438. Selth LA, Dogra SC, Rasheed MS, Healy H, Randles JW, Rezaian MA (2005) A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication. Plant Cell 17:311-325. Sera T (2005) Inhibition of virus DNA replication by artificial zinc finger proteins. J Virol 79:2614-2619. Settlage SB, See RG, Hanley-Bowdoin L (2005) Geminivirus C3 protein: replication enhancement and protein interactions. J Virol 79:9885-9895. Shepherd DN, Mangwende T, Martin DP, Bezuidenhout M, Thomson JA, Rybicki EP (2007a) Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants. J Gen Virol 88:325-336. Shepherd DN, Mangwende T, Martin DP, Bezuidenhout M, Kloppers FJ, Carolissen CH, Monjane AL, Rybicki EP, Thomson JA (2007b) Maize streak virus-resistant transgenic maize: a first for Africa. Plant Biotechnol J 5:759-767. Shivaprasad P, Thillaichidambaram P, Balaji V, Veluthambi K (2006) Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco. Virus Genes 33:365-374. Sinisterra XH, Polston NJE, Abouzid AM, Hiebert E (1999) Tobacco plants transformed with a modified coat protein of tomato mottle begomovirus show resistance to virus infection. Phytopathology 89:701-706. Smith NA, Singh SP, Wang MB, Stoutjesdijk PA, Green AG, Waterhouse PM (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407:319-320. Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In Virus Taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses, pp. 301-326. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press. Stanley J, Frischmuth T, Ellwood S (1990) Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proc Natl Acad Sci USA 87:6291-6295. Stenger DC (1994) Strain-specific mobilization and amplification of a transgenic defective-interfering DNA of the geminivirus beet curly top virus. Virology 203:397-402. Sunter G, Bisaro DM (1992) Transactivation of geminivirus AR1 and BR1 gene expression by the AL2 gene product occurs at the level of transcription. Plant Cell 4:1321-1331. Sunter G, Sunter J, Bisaro DM (2001) Plants expressing Tomato golden mosaic virus AL2 or beet curly top virus L2 transgenes show enhanced susceptibility to infection by DNA and RNA viruses. Virology 285:59-70. Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532:75-79. Takenaka G, Koshino-Kimura Y, Aoyama Y, Sera T (2007) Inhibition of tomato yellow leaf curl virus replication by artificial zinc-finger proteins. Nucleic Acids Symp Ser 51:429-430. Tripathi S, Varma A (2002) Eco-friendly management of leaf curl disease of tomato. Indian 55:473-478. Trinks D, Rajeswaran R, Shivaprasad PV, Akbergenov R, Oakeley EJ, Veluthambi K, Hohn T, Pooggin M (2005) Suppression of RNAsilencing by a geminivirus nuclear protein, AC2, correlates with transactivation of host genes. J Virol 79:2517-2527. Wang H, Hao L, Shung C-Y, Sunter G, Bisaro DM (2003) Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins. Plant Cell 15:3020-3032. Wassenegger M, Pelissier T (1998) A model for RNA-mediated gene silencing in higher plants. Plant Mol Biol 37:349-362. Waterhouse PM, Wang M-B, Lough T (2001) Gene silencing as an adaptive defence against viruses. Nature 411:834-842. Wenck A, Czako M, Kanevski I, Marton L (1997) Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation. Plant Mol Biol 34:913-922. Wijkamp I, Vvan Lent J, Kormelink R, Goldbach R, Peters D (1993) Multiplication of tomato spotted wilt virus in its vector, Frankliniella occidentalis. J Gen Virol 74:341-349. Wilson TMA (1993) Strategies to protect crop plants against viruses pathogen-derived resistance blossoms. Proc Natl Acad Sci USA 90:134-3141. Wintermantel WM, Zaitlin M (2000) Transgene translatability increase effectiveness of replicase-mediated resistance to Cucumber mosaic virus. J Gen Virol 81:587-595. Wolfenbarger LL, Phifer PR (2000) The ecological risks and benefits of genetically engineered plants. Science 290:2088-2093. van den Boogaart T, Lomonossoff GP, Davies JW (1998) Can we explain RNA-mediated virus resistance by homology-dependent gene silencing? Mol Plant-Microbe Interact 11:717-723. Vanderschuren H, Akbergenov R, Pooggin MM, Hohn T, Gruissem W, Zhang P (2007) Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs. Plant Mol Biol 64:549-557. Vanderschuren H, Alder A, Zhang P, Gruissem W (2009) Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava. Plant Mol Biol 70:265-272. Vanitharani R, Chellappan P, Fauquet CM (2005) Geminiviruses and RNA silencing. Trends Plant Sci 10:144-151. Vanitharani R, Chellappan P, Fauquet C (2003) Short interfering RNA mediated interference of gene expression and viral DNA accumulation in cultured plant cells. Proc Natl Acad Sci USA 100:9632-9636. Vanitharani R, Chellappan P, Pita JS, Fauquet C (2004) Differential roles of AC2 and AC4 of cassava geminiviruses in mediating synergism and suppression of posttranscriptional gene silencing. J Virol 78:9487-9498. van Wezel R, Liu H, Tien P, Stanley J, Hong Y (2001) Gene C2 of the monopartite geminivirus tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus. Mol Plant-Microbe Interact 14:1125-1128. van Wezel R, Liu H, Tien P, Stanley J, Hong Y (2002) Mutation of three cysteine residues in tomato yellow leaf curl virus-China C2 protein causes dysfunction in pathogenesis and posttranscriptional gene silencing-suppression. Mol Plant-Microb Interact 15:203-208. Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145-164. Vaucheret H, Christophe B, Elmayan T, Feuerbach F, Godon C, Morel JB, Mourrain P, Palauqui JC, Vernhettes S (1998) Transgene-induced gene silencing in plants. Plant J 16:651-659. Verhoog H (2003) Naturalness and the genetic modification of animals. Trends Biotechnol 21:294-297. Voinnet O, Pinto YM, Baulcombe DC (1999) Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci USA 96:14147-14152. Von Arnim A, Stanley J (1992) Inhibition of African cassava mosaic virus systemic infection by a movement protein from the related geminivirus tomato golden mosaic virus. Virology 187:555-564. Yang Y, Sherwood TA, Patte CP, Hiebert E, Polston JE (2004) Use of tomato yellow leaf curl virus (TYLCV) Rep gene sequences to engineer TYLCV resistance in tomato. Phytopathology 94:490-496. Yoder JI, Goldsbrough AP (1994) Transformation systems for generating marker-free transgenic plants. Bio/Technology 12:263
摘要: 摘要 由粉蝨傳播的雙生病毒與由薊馬所傳播的番茄萎凋病毒是目前極為重要的兩群植物病毒。利用基因工程技術藉由基因轉殖的方式為目前有效的抗病毒的策略之一。然而在轉殖的過程中,通常都會使用抗生素抗性基因或殺草劑抗性基因作為篩選用的標誌基因以區分其中少數具有轉基因的植物。但是站在生態與食品安全的角度考量,對於使用此類抗性基因的安全疑慮越來越受大眾矚目,因此本論文的主旨即是建構可穩定移除標誌基因的轉殖系統,並利用此系統生產可抗雙生病毒與番茄萎凋病毒的無篩選標誌基因之轉基因植物。本研究所分析的雙生病毒是主要危害台灣番茄生長的台灣番茄捲葉病毒,首先,為了得知基因片段中何者可提供轉基因植物對雙生病毒表現較高的抗性,因此將台灣番茄捲葉病毒全基因體分割為數個基因片段後,再分別轉殖至圓葉菸草中進行抗性分析,發現其中四個構築可提供轉基因植物較高的抗性分別為:IRC1(基因間區域與C1開放讀碼區5′ 端之序列),C2 (部分C2開放讀碼區之序列),C2C3 (C2與C3開放讀碼區重疊區域之序列) 以及Rep2 (C1開放讀碼區之3′ 端序列);並藉偵測轉基因植物中所累積的siRNA確認其抗病機制是經由基因沉寂所誘發。除此之外,番茄斑萎病毒是屬於Tospovirus屬而且擁有最廣泛寄主範圍的植物病毒之一,故此研究利用其N基因片段與台灣番茄捲葉病毒的部分C2開放讀碼區連結在一起作為一個嵌合轉基因,再將此構築轉殖入圓葉菸草與番茄中,以期能發展具有多重抗性之轉基因植物。經抗性分析後發現轉基因植物以農桿菌接種台灣番茄捲葉病毒後不會產生病徵,而機械接種番茄斑萎病毒後亦呈現高度抗性的現象,進一步分析抗病的轉基因植物則可偵測到siRNA的累積,證明此抗性是經由基因沉寂的機制所提供。同時也證實藉由連結不同基因體組成的病毒基因片段的策略可提供轉基因植物對DNA與RNA病毒具有抗性。與此同時,本論文亦以共轉殖法發展出三種生產無篩選標誌基因轉基因植物的轉殖系統,包括 (1) pGANP-CP1/pBin19:單一菌株內兩個獨立的質體各自攜帶有目標基因或標誌基因的T-DNA;(2) pGA2T-CP1:同一質體中同時帶有目標基因及標誌基因的T-DNA;(3) 為了發展可適用更多植物種類的載體,尤其是對kanamycin敏感度較低的作物,因此構築了pGA2TNH:單載體攜帶兩組T-DNA而於其中一組T-DNA中帶有兩種不同的選擇性標誌基因。經此三種系統再生之轉基因菸草的共轉型效率皆很相近,約為於50%。至於兩組T-DNA於子代的分離現象亦符合預期,於單一標誌基因重複數的轉基因植物中,移除標誌基因的比例在雙載體系統為24.1%,單載體系統可達17.5%~18.6%。結果顯示這些系統確實可行且同樣都能有效率地移除選擇性標誌基因,進一步可提供簡便及實用性兼具的工具應用至生產無篩選標誌基因的轉基因植物。因此,本論文將先前研究結果所提及之可提供轉基因植物對台灣番茄捲葉病毒產生較高抗性的基因片段,包括IRC1、C2、C2C3、Rep2並與番茄斑萎病毒之部分N基因片段連結在一起後,構築至二位元載體pGA2TNH藉此生產具有病毒抗性的無篩選標誌基因轉基因菸草。並利用農桿菌接種法篩選出對台灣番茄捲葉病毒具有抗性的轉基因親本植物,且經由自交的方式於其子代中獲得無篩選標誌基因轉基因抗病菸草。總而言之,本論文的結果提供了可應用在田間防治雙生病毒與番萎凋病毒的轉基因策略。而所研發的生產無選擇性標誌基因轉基因植物的轉殖系統,也將有助於提升一般大眾對轉基因作物的接受度。
Abstract Whitefly-transmitted geminiviruses (Geminiviridae) and thrips-borne tospoviruses (Bunyaviridae) are two groups of extremely important plant viruses. Current transgenic approach based on genetic engineering technology provides an efficient strategy to breed plants to resist viral infection. However, public concerns about the use of antibiotic- and herbicide-resistance genes for the selection of transgenic plants during the transformation process have increased tremendously. Therefore, the objectives of this study were to develop the reliable transformation system that could remove selectable markers while generating transgenic plants that would resist the infection of geminiviruses and tospoviruses. Firstly, Tomato leaf curl Taiwan virus (ToLCTWV), a predominant tomato-infecting geminivirus in Taiwan, was subjected to investigate which viral gene fragments can confer high resistance to geminiviruses in transgenic plants. Individual transgenic constructs covering the entire ToLCTWV genome was transformed into Nicotiana benthamiana plants. Four constructs including IRC1 (intergenic region flanked with 5' end of C1), C2 (partial C2 ORF), C2C3 (overlapping region of C2 and C3 ORFs) and Rep2 (3' end of the C1 ORF) of high resistance for ToLCTWV have been observed. The detection of siRNA in transgenic plants confirmed that the mechanism of resistance was via gene silencing. Moreover, the middle half of the N gene of Tomato spotted wilt virus (TSWV), which is the type member of Tospovirus, was fused with the partial C2 ORF of ToLCTWV as the chimeric transgene and transformed into N. benthamiana and tomato to develop transgenic plants with multiple viral resistance. The transgenic plants remained symptomless post agro infected with ToLCTWV and exhibited high resistance to TSWV. The detectable siRNAs demonstrated that the resistance was mediated by gene silencing mechanism. The results also explained that linking multiple gene fragments of two viruses with different genomic organization was an effective strategy to engineer plants against both DNA and RNA viruses. Meanwhile, we developed three strategies of co-transformation to generate marker-free transgenic plants; they were (1) pGANP-CP1/pBin19, which comprises two individual plasmids carrying T-DNA of the target and marker genes separately; (2) pGA2T-CP1, which consists of one plasmid carrying two T-DNAs for the target and marker genes; and (3) pGA2TNH, which contains two T-DNAs in one plasmid in which one T-DNA carries the bi-selectable marker which can be used for more plant species especially those with low sensitivity to kanamycin. The co-transformation frequencies of the R0 transgenic N. benthamiana plants for both selection marker and target gene were similar. The co-transformation frequencies of three vector systems were about 50%. Segregation of transgene and selectable marker gene was revealed in the progeny of some co-transformed lines. The highest production ratio of marker-free transgenic plants was 24.1% in two plasmids system, followed by 18.6% in one plasmid system and 17.5% in bi-selectable marker system. We demonstrated that these strategies were feasible and efficient to eliminate the marker genes, and can provide a practical and simple tool for generating marker-free transgenic plants. Therefore, previously mentioned gene fragments that confer high resistance to ToLCTWV including IRC1, C2, C2C3 and Rep2 were linked together and fused with the middle half of the N gene from TSWV to make a chimeric transgene to be constructed into the binary vector, pGA2TNH, for the generation of viral resistance marker-free transgenic N. benthamiana. The transgenic R0 plants resistant to ToLCTWV were obtained and the marker-free resistant progeny plants were segregated by self-pollination. Overall, the results showed in this study have important implications for field deployment of transgenic strategies to control geminivirus and tospovirus. Moreover, the plant transformation systems that can generate marker-free plants would certainly boost the public acceptance of transgenic crops.
其他識別: U0005-2308201010472700
Appears in Collections:植物病理學系



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