Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31516
標題: 檬果黑斑病菌之特性與其在檬果葉組織顯微鏡觀察
CHARACTERISTICS OF XANTHOMONAS CAMPESTRIS PV. MAGIFERAEIDICAE AND THE MICROSCOPY OF THIS PATHOGEN IN AND ON MANGO LEAVES
作者: 曾珍
ZENG, ZHEN
關鍵字: PLANT-PATHOLOGY
檬果黑斑病菌
BOTANY
ENTOMOLOGY
檬果葉組織
顯微鏡
植物病理
植物學
昆蟲學
出版社: 植物病理學研究所
摘要: 由台灣南部地區所分離之21個Xanthomonas campestris pv. giferaeindicae 菌株,為桿狀,具有單一極生鞭毛之格蘭式陰性菌以 Biolog GN Microplate測試對95種碳素源之利用,顯示可利用之碳素有35 種,不能利用之碳素原有33種,中間型有11種,菌株間有差異者有16種,在 PDA培養基上有3個菌株對600 ppm之硫酸銅具抗性,有2個菌株對對200ppm 鏈黴素具抗性,波爾多液8-8式,鹼性氯氧化銅(200X),與三元硫酸銅(500 X),對所有菌株皆有抗性,而快得寧與護粒丹對大部份菌株之生長已無抑制 作用.在套袋保持高濕度,有人為穿刺傷口之葉片於接種後15天傷口處菌量 達1.5 x 10^7 cfu/leaf disk,在無人為傷口之葉片菌量只達1.4x10^5 cfu/leaf disk, 在無套袋處理有傷口處之菌量亦只達 1.2x10^5 cfu/ leaf disk.以光學電顯及穿透式電顯觀察檬果黑斑病菌自傷口侵入葉片之 情形,顯示細菌會在細胞間隙增殖,破壞中膠層,使細胞分離,細胞壁破壞, 細胞內含物顆粒化,胞器變形,最後細胞崩解細菌充滿於崩解後之空腔.由 掃描式電顯觀察接種後之葉表組織可見細菌在傷口處增殖,在套袋處理之 葉片於接種後11天可見大量細菌群聚於傷口附近之組織.此些菌常存於葉 表黏稠之基質,而細菌與細菌間常見有纖維狀物質將其纏住. Twenty-one strains of Xanthomonas campestris pv. mangiferaeindicae(abbreviated to Xcm)isolated from southernaiwan were characterized. They were Gram-negative rods with a single polar flagellum. Utilization of 95 various carbon sources was examined on Biolog GN Microplates.All strains were positive for utilization of 35 and negative for 33 carbon sources tested.Inaddition, borderline reaction was observed for 11 carbon sources,strains varied with the ablity to utilize of 16 carbon sources.On PDA plates,3strains of Xcm were resist Infection of mango leaves through wound by Xcm was observed by light microscopy and trasmission and scanning electron microscopies. It revealed that the pathogen enter and multiplied in the intercellular spaces, dissolution of the middle lamella were observed 24 hr after inoculation. Infected host cells gradually become separated. Condensation of cellular content and disorganization of orgenelles were observed during the progress of the infection. Eventully the infected host cells wereisintegrated. Numerous bacteria were found in the lysigenousty
URI: http://hdl.handle.net/11455/31516
Appears in Collections:植物病理學系

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