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標題: 以反應曲面法最適化改質尼龍菜瓜布作為固定化金屬螯合親和吸附材之研究
Optimization of modified nylon sponges as immobilized metal ion affinity adsorbents using response surface methodology
作者: 陳韻如
Chen, Yun-Ru
關鍵字: 尼龍菜瓜布
nylon sponge
response surface methodology
immobilized metal ion affinity adsorbent
出版社: 化學工程學系所
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摘要: 本研究利用改質方式使尼龍菜瓜布表面改質(鹽酸水解) 、表面活化(戊二醛、己二胺、環氧氯丙烷) ,之後接上螯合劑和金屬離子 (Cu 2+ )後,即完成固定化金屬親和螯合吸附材之製備,並利用反應曲面法 (Response Surface Methodology, RSM) 探討鹽酸水解時間、戊二醛濃度、己二胺濃度對銅離子鍵結量之影響,以中心混成實驗找到吸附材合適的最佳參數條件。 從實驗結果發現尼龍菜瓜布經過改質後,當參數條件設為鹽酸水解時間 2.64 小時、戊二醛濃度 15.33%、己二胺濃度 0.19 M 時,銅離子鍵結量可達4.247 mmol/g 。在中心混成實驗部分,經由數學軟體統計回歸計算可得知,在陡升路徑實驗中選擇的範圍區域是正確的。 最後,利用製備出的吸附基材進行蛋白質 (2 – Epimerase) 吸附實驗,在蛋白吸附及脫附方面,判斷在 Flow through 部分的銅離子沉澱吸附了大部分的標的蛋白,導致實驗後續的 Wash、 Elution、 SDS-boiling 部分無法如預期清洗雜蛋白、脫附標的蛋白。
In this study, modified nylon sponges were prepared by first reacting original sponges sequentially with hydrochloric acid, glutaraldehyde, hexanediamine and epichlorohydrin. After practicing those procedures mentioned above, nylon sponges were then coupled with IDA (iminodiacetic acid) and loaded with Cu2+ to attain immobilized metal affinity (IMA) absorbents. The effect of hydrochloric acid hydrolysis time, glutaraldehyde concentration and hexanediamine concentration on the amount of chelated metal ion were analyzed by RSM (Response Surface Methodology) to decide the optimum values of independent factors for the best response value. Under optimnm conditions, hydrochloric acid hydrolysis time 2.64 hr, glutaraldehyde concentration 15.33%, and hexanediamine concentration 0.19 M, the amount of immobilized Cu2+ could reach up to 4.247 mmol/g. The results from RSM reveal the reliability for this study. At last, the results showed that there were Cu2+ precipitations in the buffer when adsorbing protein on prepared IMA absorbents. That is the reason for unpredicted experimental results.
其他識別: U0005-1308201217433600
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