Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3162
標題: 以反應曲面法最適化改質尼龍菜瓜布作為固定化金屬螯合親和吸附材之研究
Optimization of modified nylon sponges as immobilized metal ion affinity adsorbents using response surface methodology
作者: 陳韻如
Chen, Yun-Ru
關鍵字: 尼龍菜瓜布
nylon sponge
反應曲面法
固定化金屬螯合親和吸附材
response surface methodology
immobilized metal ion affinity adsorbent
出版社: 化學工程學系所
引用: 李晏忠。利用蛋白質之工程技術改造 N-acetyl D-glucosamine 2- epimerase 及 N-acetyl-neuraminate aldolase 的性質。工業技術研究院委託學術機構研究報告,化學工程研究所,國立中興大學,2000。 劉哲良。聚甲基丙烯酸二羥基乙酯固定化金屬親和吸附材於蛋白質純 化之應用。碩士論文,化學工程研究所,國立中興大學,2002。 黃銘道。尼龍改質固定化金屬親和吸附材於蛋白質純化之應用。碩士論文,化學工程研究所,國立中興大學,2003。 陳怡君。聚乙烯醇固定化金屬親和吸附材於蛋白質純化之應用。碩士論文,化學工程研究所,國立中興大學,2008。 邱美雪。以聚乙烯醇修飾 PET 不織布做為固定化金屬螯合親和吸附材之研究。碩士論文,化學工程研究所,國立中興大學,2010。 Aktaş, N., İ. H. Boyacı, M. Mutlu, and A. Tanyolac, 2006, Optimization of lactose utilization in deproteinated whey by Kluyveromyces marxianus using response surface methodology (RSM), Bioresource Technology 97, 2252-2259. Alvarez, C., M. Strumia, and H. Bertorello, 2001, Synthesis and characterization of a biospecific adsorbent containing bovine serum albumin as a ligand and its use for bilirubin retention, Journal of Biochemical and Biophysical Methods 49, 649-656. Alvarez, C. I., C. G. Gomez, and M. C. Strumia, 2001, Influence of the polymeric morphology of sorbents on their properties in affinity chromatography, Journal of Biochemical and Biophysical Methods 49, 141-151. Alvarez, C. I., M. C. Strumia, and H. E. Bertorello, 1997, Preparation of adsorbents applicable to pseudobiospecific ligand affinity chromatography using different spacers and ligands, Reactive & Functional Polymers 34, 103-111. Anderson-Cook, C. M., C. M. Borror, and D. C. Montgomery, 2009, Response surface design evaluation and comparison, Journal of Statistical Planning and Inference 139, 629-641. Armisen, P., C. Mateo, E. Cortes, J. L. Barredo, F. Salto, B. Diez, L. Rodes, J. L. Garcia, R. Fernandez-Lafuente, and J. M. Guisan, 1999, Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports, Journal of Chromatography A 848, 61-70. Arnau, J., C. Lauritzen, G. E. Petersen, and J. Pedersen, 2006, Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins, Protein expression and purification 48, 1-13. Beeskow, T. C., W. Kusharyoto, F. B. Anspach, K. H. Kroner, and W. D. Deckwer, 1995, Surface Modification of Microporous Polyamide Membranes with Hydroxyethyl Cellulose and Their Application as Affinity Membranes, Journal of Chromatography A 715, 49-65. Bezerra, M. A., R. E. Santelli, E. P. Oliveira, L. S. Villar, and L. A. Escaleira, 2008, Response surface methodology (RSM) as a tool for optimization in analytical chemistry, Talanta 76, 965-977. Block, H., B. Maertens, A. Spriestersbach, N. Brinker, J. Kubicek, R. Fabis, J. Labahn, and F. Schafer, 2009, Immobilized-Metal Affinity Chromatography (Imac): A Review, Guide to Protein Purification, Second Edition 463, 439-473. Boden, V., J. J. Winzerling, M. Vijayalakshmi, and J. Porath, 1995, Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography, J Immunol Methods 181, 225-232. Bornhorst, J. A., and J. J. Falke, 2000, Purification of proteins using polyhistidine affinity tags, Applications of Chimeric Genes and Hybrid Proteins, Pt A 326, 245-254. Burton, S. C., and D. R. K. Harding, 2001, Salt-independent adsorption chromatography: new broad-spectrum affinity methods for protein capture, Journal of Biochemical and Biophysical Methods 49, 275-287. Carlsson, J., J. Porath, and B. Lonnerdal, 1977, Isolation of lactoferrin from human milk by metal-chelate affinity chromatography, Febs Letters 75, 89-92. Chaga, G. S., 2001, Twenty-five years of immobilized metal ion affinity chromatography: past, present and future, Journal of Biochemical and Biophysical Methods 49, 313-334. Chang, S. F., S. W. Chang, Y. H. Yen, and C. J. Shieh, 2007, Optimum immobilization of Candida rugosa lipase on Celite by RSM, Applied Clay Science 37, 67-73. Chen, W. Y., C. F. Wu, and C. C. Liu, 1996, Interactions of imidazole and proteins with immobilized Cu(II) ions: Effects of structure, salt concentration, and pH in affinity and binding capacity, Journal of Colloid and Interface Science 180, 135-143. Clonis, Y. D., 2006, Affinity chromatography matures as bioinformatic and combinatorial tools develop, Journal of Chromatography A 1101, 1-24. Denizli, A., and E. Piskin, 2001, Dye-ligand affinity systems, Journal of Biochemical and Biophysical Methods 49, 391-416. Denizli, A., B. Salih, S. Senel, and M. Y. Arica, 1998, New metal chelate sorbent for albumin adsorption: Cibacron blue F3GA-Zn(II) attached microporous poly(HEMA) membranes, Journal of Applied Polymer Science 68, 657-664. Edy, V. G., A. Billiau, and P. de Somer, 1977, Purification of human fibroblast interferon by zinc chelate affinity chromatography, J Biol Chem 252, 5934-5935. el Rassi, Z., and C. Horvath, 1986, Metal chelate-interaction chromatography of proteins with iminodiacetic acid-bonded stationary phases on silica support, J Chromatogr 359, 241-253. Fan, G. J., Y. B. Han, Z. X. Gu, and D. M. Chen, 2008, Optimizing conditions for anthocyanins extraction from purple sweet potato using response surface methodology (RSM), Lwt-Food Science and Technology 41, 155-160. Gaberc-Porekar, V., and V. Menart, 2001, Perspectives of immobilized-metal affinity chromatography, Journal of Biochemical and Biophysical Methods 49, 335-360. Garg, U. K., M. P. Kaur, V. K. Garg, and D. Sud, 2008, Removal of Nickel(II) from aqueous solution by adsorption on agricultural waste biomass using a response surface methodological approach, Bioresource Technology 99, 1325-1331. Gort, S., and S. Maloy, 1998, Purification of a hexahistidine-tagged protein using L-histidine as the eluent, Technical Tips Online 3, 54-55. Gunawan, E. R., M. Basri, M. B. A. Rahman, A. B. Salleh, and R. N. Z. A. Rahman, 2005, Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester, Enzyme and Microbial Technology 37, 739-744. Idris, A., F. Kormin, and M. Y. Noordin, 2006, Application of response surface methodology in describing the performance of thin film composite membrane, Separation and Purification Technology 49, 271-280. Irvine, G. B., 1997, Size-exclusion high-performance liquid chromatography of peptides: a review, Analytica Chimica Acta 352, 387-397. Irvine, G. B., 2003, High-performance size-exclusion chromatography of peptides, Journal of Biochemical and Biophysical Methods 56, 233-242. Isgrove, F. H., R. J. H. Williams, G. W. Niven, and A. T. Andrews, 2001, Enzyme immobilization on nylon-optimization and the steps used to prevent enzyme leakage from the support, Enzyme and Microbial Technology 28, 225-232. Itoh, T., B. Mikami, I. Maru, Y. Ohta, W. Hashimoto, and K. Murata, 2000, Crystal structure of N-acyl--glucosamine 2-epimerase from porcine kidney at 2.0 A resolution1, Journal of Molecular Biology 303, 733-744. Labrou, N. E., 2003, Design and selection of ligands for affinity chromatography, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 790, 67-78. Lebreton, J. P., 1977, Purification of the human plasma alpha2-SH glycoprotein by zinc chelate affinity chromatography, Febs Letters 80, 351-354. Lee, V. A., R. G. Craig, F. E. Filisko, and R. Zand, 1996, Preparation and characterization of high-surface-area polymer substrates for microcalorimetry, J Biomed Mater Res 31, 51-62. Lee, W. C., S. Yusof, N. S. A. Hamid, and B. S. Baharin, 2006, Optimizing conditions for enzymatic clarification of banana juice using response surface methodology (RSM), Journal of Food Engineering 73, 55-63. Lienqueo, M. E., A. Mahn, J. C. Salgado, and J. A. Asenjo, 2007, Current insights on protein behaviour in hydrophobic interaction chromatography, J Chromatogr B Analyt Technol Biomed Life Sci 849, 53-68. Liesiene, J., K. Racaityte, M. Morkeviciene, P. Valancius, and V. Bumelis, 1997, Immobilized metal affinity chromatography of human growth hormone. Effect of ligand density, J Chromatogr A 764, 27-33. Luo, Q. Z., H. F. Zou, X. Z. Xiao, Z. Guo, L. Kong, and X. Q. Mao, 2001, Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate), Journal of Chromatography A 926, 255-264. Mezarosova, K., G. Tishchenko, K. Bouchal, and M. Bleha, 2003, Immobilized-metal affinity sorbents based on hydrophilic methacrylate polymers and their interaction with immunoglobulins, Reactive & Functional Polymers 56, 27-35. Mundra, P., K. Desai, and S. S. Lele, 2007, Application of response surface methodology to cell immobilization for the production of palatinose, Bioresource Technology 98, 2892-2896. Murza, A., R. Fernandez-Lafuente, and J. M. Guisan, 2000, Essential role of the concentration of immobilized ligands in affinity chromatography: purification of guanidinobenzoatase on an ionized ligand, J Chromatogr B Biomed Sci Appl 740, 211-218. Porath, J., 1988, IMAC—Immobilized metal ion affinity based chromatography, TrAC Trends in Analytical Chemistry 7, 254-259. Raissi, S., 2009, Developing new processes and optimizing performance using response surface methodology, World Academy of Science, Engineering and Technology 49, 1039-1042. Shi, Q. H., Y. Tian, X. Y. Dong, S. Bai, and Y. Sun, 2003, Chitosan-coated silica beads as immobilized metal affinity support for protein adsorption, Biochemical Engineering Journal 16, 317-322. Silva, C., C. J. Silva, A. Zille, G. M. Guebitz, and A. Cavaco-Paulo, 2007, Laccase immobilization on enzymatically functionalized polyamide 6, 6 fibres, Enzyme and Microbial Technology 41, 867-875. Tang, J., N. He, L. Nie, P. Xiao, and H. Chen, 2004, Hydrolysis of microporous polyamide-6 membranes as substrate for in situ synthesis of oligonucleotides, Surface Science 550, 26-34. Thiemann, J., J. Jankowski, J. Rykl, S. Kurzawski, T. Pohl, B. Wittmann-Liebold, and H. Schluter, 2004, Principle and applications of the protein-purification-parameter screening system, Journal of Chromatography A 1043, 73-80. Tsai, Y. H., M. Y. Wang, and S. Y. Suen, 2002, Purification of hepatocyte growth factor using polyvinyldiene fluoride-based immobilized metal affinity membranes: equilibrium adsorption study, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 766, 133-143. Ueda, E. K. M., P. W. Gout, and L. Morganti, 2003, Current and prospective applications of metal ion-protein binding, Journal of Chromatography A 988, 1-23. Vunnum, S., S. Gallant, and S. Cramer, 1996, Immobilized metal affinity chromatography: displacer characteristics of traditional mobile phase modifiers, Biotechnology Progress 12, 84-91. Westra, D. F., G. W. Welling, D. G. Koedijk, A. J. Scheffer, T. H. The, and S. Welling-Wester, 2001, Immobilised metal-ion affinity chromatography purification of histidine-tagged recombinant proteins: a wash step with a low concentration of EDTA, J Chromatogr B Biomed Sci Appl 760, 129-136. Wu, C. F., W. Y. Chen, and J. F. Lee, 1996, Microcalorimetric studies of the interactions of imidazole with immobilized Cu(II): Effects of pH value and salt concentration, Journal of Colloid and Interface Science 183, 236-242. Wu, C. Y., S. Y. Suen, S. C. Chen, and J. H. Tzeng, 2003, Analysis of protein adsorption on regenerated cellulose-based immobilized copper ion affinity membranes, Journal of Chromatography A 996, 53-70. Zhang, S., and Y. Sun, 2002, Study on protein adsorption kinetics to a dye-ligand adsorbent by the pore diffusion model, J Chromatogr A 964, 35-46.
摘要: 本研究利用改質方式使尼龍菜瓜布表面改質(鹽酸水解) 、表面活化(戊二醛、己二胺、環氧氯丙烷) ,之後接上螯合劑和金屬離子 (Cu 2+ )後,即完成固定化金屬親和螯合吸附材之製備,並利用反應曲面法 (Response Surface Methodology, RSM) 探討鹽酸水解時間、戊二醛濃度、己二胺濃度對銅離子鍵結量之影響,以中心混成實驗找到吸附材合適的最佳參數條件。 從實驗結果發現尼龍菜瓜布經過改質後,當參數條件設為鹽酸水解時間 2.64 小時、戊二醛濃度 15.33%、己二胺濃度 0.19 M 時,銅離子鍵結量可達4.247 mmol/g 。在中心混成實驗部分,經由數學軟體統計回歸計算可得知,在陡升路徑實驗中選擇的範圍區域是正確的。 最後,利用製備出的吸附基材進行蛋白質 (2 – Epimerase) 吸附實驗,在蛋白吸附及脫附方面,判斷在 Flow through 部分的銅離子沉澱吸附了大部分的標的蛋白,導致實驗後續的 Wash、 Elution、 SDS-boiling 部分無法如預期清洗雜蛋白、脫附標的蛋白。
In this study, modified nylon sponges were prepared by first reacting original sponges sequentially with hydrochloric acid, glutaraldehyde, hexanediamine and epichlorohydrin. After practicing those procedures mentioned above, nylon sponges were then coupled with IDA (iminodiacetic acid) and loaded with Cu2+ to attain immobilized metal affinity (IMA) absorbents. The effect of hydrochloric acid hydrolysis time, glutaraldehyde concentration and hexanediamine concentration on the amount of chelated metal ion were analyzed by RSM (Response Surface Methodology) to decide the optimum values of independent factors for the best response value. Under optimnm conditions, hydrochloric acid hydrolysis time 2.64 hr, glutaraldehyde concentration 15.33%, and hexanediamine concentration 0.19 M, the amount of immobilized Cu2+ could reach up to 4.247 mmol/g. The results from RSM reveal the reliability for this study. At last, the results showed that there were Cu2+ precipitations in the buffer when adsorbing protein on prepared IMA absorbents. That is the reason for unpredicted experimental results.
URI: http://hdl.handle.net/11455/3162
其他識別: U0005-1308201217433600
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1308201217433600
Appears in Collections:化學工程學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.