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Study of nonpathogenic Fusarium oxysporum in controlling Fusarium wilt of cucumber and asparagus bean
nonpathogenic Fusarium oxysporum
Fusarium wilt of cucumber
hypocotyl cutting inoculation method
Fusarium wilt of asparagus bean
The nonpathogenic Fusarium oxysporum Fo366 had been demonstrated that it could delay the symptom expression of Fusarium wilt of cucumber by using seed coating and substrate infestation inoculation methods, but it could not guarantee a good yield to cucumber plants. The purposes of this thesis were to improve the protection efficacy by using a hypocotyl cutting inoculation method to preinoculate Fo366 into cucumber; to explore if Fo366 could be used for controlling other form species of F. oxysporum; and to study the possible mechanisms of biocontrol of Fo366. In greenhouse, three inoculation methods of seed coating, substrate infestation and hypocotyl cutting were compared on the effect of controlling Fusarium wilt of cucumber. The results indicated that the hypocotyl cutting method was the best one and its protection efficacy might last until harvest period. Moreover, Fo366 was also introduced into asparagus bean and snap bean by substrate infestation inoculation method and resulted that Fo366 performed well on inhibiting Fusarium wilt of asparagus bean, but not snap bean. Different populations of Fo366 were tested to control Fusarium wilt of asparagus bean and the results indicated that the disease control could only be achieved when the population of Fo366 was 10 times larger than that of pathogen population. In addition, Fo366 applied to cucumber roots could not delay symptom expression induced by the pathogen (F. oxysporum f. sp. cucumerinum) applied directly into the stem of plants 14 days later by using root-pruning inoculation method. Therefore, the possible mechanisms of disease protection might be the competition between pathogenic and nonpathogenic F. oxysporum for infection site or something else. Furthermore, the GUS gene was transferred into Fo366 in order to observe the distribution and colonization of Fo366 in plant tissue directly under microscope. More nonpathogenic Fusarium oxysporum isolates, Fo159, Fo276 and Fo501, (especially Fo276) had been also demonstrated that it could delay the development of Fusarium wilt of asparagus bean by using substrate infestation inoculation method.
|Appears in Collections:||植物病理學系|
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