Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31929
DC FieldValueLanguage
dc.contributor陳啟予zh_TW
dc.contributorChi-Yu Chenen_US
dc.contributor.author史香芸zh_TW
dc.contributor.authorShih, Siang-Yunen_US
dc.contributor.other植物病理學系所zh_TW
dc.date2012en_US
dc.date.accessioned2014-06-06T07:42:22Z-
dc.date.available2014-06-06T07:42:22Z-
dc.identifierU0005-1508201217554300en_US
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dc.identifier.urihttp://hdl.handle.net/11455/31929-
dc.description.abstract木層孔菌屬(Phellinus)於1886年由Quelet學者建立,早期將子實層為褐孔狀,多年生的白腐真菌歸納其中。根據Wagner and Fischer (2002)分子親緣分析後發現,木層孔菌屬為一多源性的族群,可利用核醣體LSU片段親緣關係分析細分為9個單一親緣關係之屬:Fomitiporia、Fulvifomes 、Fuscoporia、Phellinidium、Phellinopsis、Phellinus、Phellopilus、Phylloporia、Porodaedalea。以至於早期木層孔菌屬之觀念為廣義,而細分後之屬為狹義。本研究調查台灣廣義木層孔菌之種類,結合台灣日據時期之標本、自行採集標本與台灣國立自然科學博物館之真菌標本館藏,以現代分類觀念重新檢視其分類地位。兩年間一共鑑定出10種台灣新記錄種與11種已記錄種。經本研究彙整,台灣廣義木層孔菌種類新增至44種,分別歸類於8個屬中。此外,為確保形態鑑定之正確性,利用核醣體LSU片段進行親緣關係分析,發現其中Fulvifomes melleoporus之親緣關係上應屬於Fuscoporia屬,而Phellinidium屬之種類可被分支至兩個分類群。由此結果顯示Fulvifomes melleoporus可移至Fuscoporia屬中,而Phellinidium屬仍為多源性的分類族群。此外,本研究共獲得8株菌株,並進行一系列培養測試,包括培養描述、氧化能力分析與最適生長環境測試。藉由本論文對台灣廣義木層孔菌之分類與培養研究,相信對未來其他領域之延伸應用提供了具價值性之資訊。zh_TW
dc.description.abstractThe genus Phellinus sensu lato was created by Quelet in 1886 to encompass brown poroid, perennial white-rot fungi. Phylogenetic analyses (Wagner and Fischer, 2002), revealed that the genus Phellinus is actually polyphyletic, and could be subdivided into nine monophyletic genera, included: Fomitiporia, Fulvifomes, Fuscoporia, Phellinidium, Phellinopsis, Phellinus sensu stricto, Phellopilus, Phylloporia, and Porodaedalea. The aim of this study was to survey and classify the genus Phellinus sensu lato in Taiwan based on novel taxonomic concepts. Materials were from Sawada’s collections, herbarium of National Museum of Nature Science, and collections obtained during 2010 to 2012. Twenty-one species have been identified. Among them ten species are newly reported. Species of Phellinus sensu lato species in Taiwan amounts to forty-four species beologing 8 genera. To confirm the accuracy of morphological identification, phylogenetic analyses were conducted based on LSU rDNA. The results show that Fulvifomes melleoporus turned out to cluster with the genus Fuscoporia, and Phellinidium species were divided into two groups. It suggests that Fulvifomes melleoporus should be placed into the genus Fuscoporia, and the genus Phellinidium is polyphyletic. Cultural tests, including cultural description, oxidase reaction and optimal environment for growing, were conducted for eight strains. This taxonomic study on Phellinus sensu lato species will provide valuable information for further sustainable application.en_US
dc.description.tableofcontents目次 中文摘要 i Abstract ii 目次 iii 前言 1 一、廣義木層孔菌屬的分類 1 1. 廣義木層孔菌屬與狹義木層孔菌屬之概念 1 2. 廣義木層孔菌的世界性研究概況 2 3. 廣義木層孔菌在台灣的研究情形 2 二、廣義木層孔菌屬的生態 3 三、廣義木層孔菌屬之經濟重要性 4 1. 醫學上之研究與應用 4 2. 木本植物病原菌 5 四、廣義木層孔菌屬之鑑定 6 1. 形態鑑定-巨觀特徵 6 2. 形態鑑定-微觀特徵 9 五、分子生物學分類法 11 材料與方法 14 一、標本蒐集、鑑定、培養與保存 14 1. 標本與菌株來源 14 2. 野外樣本蒐集 14 3. 菌種描述要點 15 4. 菌種純化與保存 16 二、親緣關係分析 17 1. DNA萃取 17 2. 聚合酵素連鎖反應(polymerase chain reaction, PCR) 18 3. 聚合酵素連鎖反應產物純化與濃縮 19 4. 核酸電泳分析 19 5. 核酸定序 19 6. 演化樹之建立 20 三、菌株培養測試 20 1. 菌落觀察 20 2. 酚類氧化酵素活性測試 21 3. 最適生長溫度與培養基測試 21 四、註記 21 結果 23 一、日據時期鑑定之廣義Phellinus屬標本檢視 23 二、野外標本採集 23 三、屬間特徵檢索表 24 四、標本鑑定 24 五、親緣關係分析 25 六、菌株之培養,生理生化測試與培養觀察 25 一、日據時期鑑定之廣義Phellinus屬標本檢視 27 三、屬間特徵檢索表 31 四、菌種描述 33 五、親緣關係分析 81 六、菌株之培養,生理生化測試與培養觀察 82 討論 107 一、標本採集與鑑定 107 二、屬間形態區分 107 三、與廣義木層孔菌相似種類之鑑別 108 四、親緣關係分析 109 1. Fulvifomes melleoporus之分類問題 110 2. Phellinidium noxium之分類問題 110 五、菌株分離困境之突破 111 六、菌株培養測試 112 1. 菌株之抗逆境現象 112 2. 新發現之菌落鑑定之特徵 112 七、台灣記錄種的彙整 113 八、結語 113 引用文獻 115 附錄 119 Table 4 序列資料與菌株來源 120 Table 5 台灣產廣義木層孔菌之記錄種彙整 122 Table 6 採集標本資料 126zh_TW
dc.language.isozh_TWen_US
dc.publisher植物病理學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1508201217554300en_US
dc.subject廣義木層孔菌屬zh_TW
dc.subjectPhellinus sensu latoen_US
dc.subject分類zh_TW
dc.subject木材腐朽真菌zh_TW
dc.subject親緣關係分析zh_TW
dc.subjecttaxonomyen_US
dc.subjectwood-decaying fungien_US
dc.subjectphylogenetic analysisen_US
dc.title台灣產廣義木層孔菌屬之分類研究zh_TW
dc.titleTaxonomic studies of the genus Phellinus sensu lato in Taiwanen_US
dc.typeThesis and Dissertationzh_TW
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