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標題: 探討人工環境培養之葉芽線蟲病原性消失之成因
The investigation of pathogenicity decreasing of Aphelenchoides besseyi under artificial culturing conditions
作者: 温柔茜
Wen, Rour-Chiahn
關鍵字: 葉芽線蟲 Aphelenchoides besseyi
Aphelenchoides besseyi
姐妹品系 sister line
artificial environment
bird’s-nest fern
出版社: 植物病理學系所
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摘要: 本研究成功於人工培養七代後,誘導出弱病原性之Aphelenchoides besseyi族群,此族群已無法在原寄主山蘇上造成病害,並且由接種部位無法再度分離出接種線蟲。第曼測量值結果顯示,培養於人工環境中之A. besseyi族群(AC),其體長與體寬之測量值於第二至六世代皆大於培養於山蘇上之A. besseyi族群(BNF),但人工環境培養第一代之A. besseyi族群,其體長與體寬則與山蘇上之A. besseyi族群相似,兩種處理組之各子代第曼測量值皆落在Dastur (1936)發表之A. besseyi範圍內。AC族群於第二至七世代體長與體寬的改變應與人工環境中過剩之營養來源或試管中較大之生存空間有關,與病原性強弱或有無並無關係。人工環境繼代培養各子代之雌雄比與族群數目結果,顯示這兩個數據與病原性消失之間皆無共同趨勢,比較各子代之雌雄比與族群數目,結果亦無關聯性。在人工培養環境之七個世代間,雄蟲數目無明顯差異而族群數目亦無下降趨勢,表示於人工培養環境中未處於逆境,故推測AC族群病原性消失的現象並非由逆境引起。為進一步了解AC族群病原性消失的原因是由遺傳因子或環境因子改變決定,本研究取單一母蟲建立四個族群,並以不同培養環境或交替不同培養環境處理。第一次試驗結果顯示,四種不同取食環境處理之族群(AA、AB、BA、BB)於第三世代回接山蘇造成之病徵面積未有明顯差異,但第二次試驗中,持續培養於山蘇上之BB處理組造成之罹病面積在第三世代便顯著高於其他三個處理組。測量四種處理組第六代回接山蘇造成之病徵面積發現,兩次試驗中BB處理組皆造成最大罹病面積,而持續培養於人工環境之AA處理組造成之罹病面積最小。結果顯示,A. besseyi族群於人工環境培養後病原性消失之成因可能與營養來源的改變有關,且此現象需累積至少六代方可表現。
This study successfully induced low pathogenicity Aphelenchoides besseyi populations by sub-culturing on Alternaria citri PDA slants for seven generations. The low pathogenicity population could not parasitize the original host bird’s-nest fern (Asplenium nidus L.), cause any symptoms, or be re-isolated from the inoculate sites. The A. besseyi populations cultured on A. citri PDA slant (AC) has bigger body length and width at the 2nd to 7th generation than the population cultured on bird’s-nest fern (BNF), however, the 1st generation of both populations had similar body length and width. All the morphometrics taken in this study were in the range of A. besseyi measured by Dastur in 1936. The smaller body length and width of pathogenicity A. besseyi populations might due to the change of nutrients in the environment or the bigger living space on the slants. The sex ratio and the population number from seven generations of AC population did not show specific trend or correlation to the pathogenicity, the sex ratio also did not correlated with the population number. The numbers of male from the seven generations showed no significant differences, and the population number did not decrease along with the pathogenicity. All the results indicated that, the decreasing of pathogenicity has no correlation with the stress from the artificial environment. To investigate the correlation between nematode culturing conditions and the pathogenicity on bird’s-nest fern, four sister lines derived from one single female was established and sub-cultured under different conditions for six generations. In the 1st experiment repeat, the diseased areas caused by the 3rd generation of four sister lines (AA,AB,BA,BB), did not show significant differences, but in the 2nd experiment repeat BB lines caused significant bigger diseased area than other three treatments. At the 6th generation, both experiment repeats showed that BB line caused the biggest diseased area, while AA line caused the smallest. This indicated that the decreasing of pathogenicity in A. besseyi might be strongly correlated with the nutrient sources in the culturing environment.
其他識別: U0005-0808201210415700
Appears in Collections:植物病理學系



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