請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/31965
標題: 水生植物線蟲相之調查與鑑定
The survey and identification of nematodes associated with aquatic plants
作者: 黃思頴
Huang, Sih-Ying
關鍵字: 葉芽線蟲
Aphelenchoides spp.
水生植物
腐生性線蟲Chronogasteridae
獵食性線蟲Dorylaimidae
穿根線蟲
南方根瘤線蟲
花生根瘤線蟲
Aquatic plants
Chronogasteridae
Dorylaimidae
Hirschmanniella spp.
Meloidogyne incognita
Meloidogyne arenaria
出版社: 植物病理學系所
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European Journal of Plant Pathology 108: 497-506. Bridge, J., Richard, A. P. and Deliang, P. 2005. Nematode Parasites of Rice. pp.87-118 in: Luc, M., Sikora, R. A. and Bridge, J. Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd edition. CAB International. Wallingford. UK. pp.871. Brunel, S. 2009. Pathway analysis: aquatic plants imported in 10 EPPO countries. OEPP/EPPO Bulletin 39:201-213. Coursey, D. 1995. Exotic Aquatic Plants. Strategic Assessment of Florida''s Environment-Florida Center for Public Management. Website: http://www.pepps.fsu.edu/safe/pdf/ale9.pdf. 2012/06/24 Dixon, W. N. and Inserra, R. N. 1998. Tri-ology 37(5). Florida Department of Agriculture and Consumer Services. Website: http://www.freshfromflorida.com/pi/enpp/triology/archive/98-sep-oct.htm. 2012.06.25 EPPO. 2004a. EPPO report on notifications of non-compliance(detection of regulated pests). EPPO Reporting Service. Paris 1:11-15 EPPO. 2004b. 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Effects of Hirschmanniella caudacrena on submerged aquatic plants Ceratophyllum demersum and Hydrilla verticillata. Journal of Nematology 19(4):447-453. Inserra, R. N. 1998. Tri-ology Technical Report. 37(1):7 Nematology Section. Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL, USA. Lancar, L. and Krake, K. 2002. Aquatic Weeds & their Management. International Commission on Irrigation and Drainage. Lehman, P. S., Vovlas, N., Inserra, R. N., Duncan, L. W. and Kaplan, D. T. 2000. Colonization of foliar tissues of an aquatic plant, Anubias barteri Schott, by Radopholus similis. Nematropica 30:63-57. Karajeh, M. R., Abu-Gharbieh, W. I. and Masoud, S. A. 2010. DNA Extraction and PCR-Based Diagnosis of the Root-Knot Nematodes (Meloidogyne Species and Races) of Jordan. Jordan Journal of Agricultural Sciences 6(3):342-352. Paul, D. L. 2003. Identification of Free-living nematodes(Secernentea). University of California, Riverside Universiry Extension Peterson, D. E. and Lee, C. 2005. Aquatic Plants and Their Control. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Website: www.oznet.ksu.edu. 2012/05/01 Powers, T. O. and Harris, T. S. 1993. A polymerase chain reaction method for identification of five major Meloidogyne Species. Journal of Nematology 25(1):p.1-6. Poinar, G. O. and Sarbu, S. M. 1994. Chronogaster troglodytes sp. n. (Nemata : Chronogasteridae) from Movile Cave, with a review of cavernicolous nematodes. Fundamental and applied nematology. 17(3): 231-237. Ravichandra, N. G. 2008. Plant Nematology. I. K. International. New Delhi. Inida. p.149, 153-154. Rosamond, L. N., Susan, L. W. and Donald, R. S. 2001. Aquaculture—A Gateway for Exotic Species. Science 294: 1655-1656. Ryss, A. Y. and Karnkowski, W. 2010. Observations on Radopholus bridgei Siddiqi& Hahn, 1995 intercepted in exotic aquarium plants imported to Poland. 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摘要: 本研究針對台灣20個分類科雙子葉植物以及5個科單子葉植物,共76種市售水草進行線蟲相調查,調查對象包括腐生性線蟲、獵食性線蟲以及植物寄生性線蟲。結果顯示共有6個分類科腐生性線蟲以及4個分類科之獵食性線蟲由雙子葉植物調查出,包括腐生性線蟲Chronogasteridae, Diplopeltidae, Leptolaimidae, Mesorhabditidae, Rhabditidae和Tripylidae科; 獵食性線蟲的Actinolaimidae, Anatonchidae, Cyanotholaimidae和Dorylaimidae科,植物寄生性線蟲則僅有葉芽線蟲屬被發現,其中黃菊花草上可分離到的葉芽線蟲數量最高。單子葉植物線蟲相調查結果發現8個分類科之腐生性線蟲,包括: Cephalobidae, Chronogasteridae, Leptopaimidae, Mesorhabditidae, Panagrolaimidae, Plectidae, Rhabditidae和Tripylidae科,以及9個科之獵食性線蟲: Actinolaimidae, Aporcelaimidae, Belondiridae, Dorylaimidae, Metateratocephalidae, Mononchidae, Mylonchulidae, Sphaerolaimidae和Tylencholaimellidae科,植物寄生性線蟲除了葉芽線蟲屬外,亦有穿根線蟲屬(Hirschmanniella)以及根瘤線蟲屬(Meloidogyne)被調查出。本研究調查結果顯示腐生性線蟲的種類以Chronogasteridae科出現頻率最高; 獵食性線蟲則以Dorylaimidae科出現次數最多,推測因水生植物可提供該兩種線蟲族群主要食物來源,故使線蟲族群大量繁衍。僅有三屬植物寄生性線蟲由水生植物分離出,葉芽線蟲經由形態觀察後發現與楊氏發表之葉芽線蟲新種相似。穿根線蟲因只分離出兩隻,故只能將其分類地位鑑定至屬。根瘤線蟲於榕類植物根系上可造成明顯結瘤,雌蟲經由陰門膜紋鑑定後發現其膜紋紋路緊密,拱弧低且紋路雜亂,不具有明顯側線,肛門上方無點狀物,推測為花生根瘤線蟲(M. arenaria)。此外,增幅五個純系根瘤線蟲族群COII區間,皆可得一1700bp大小之條帶,可確定其為南方、花生或爪哇根瘤線蟲其中一種。再利用SCAR引子對進一步確認根系中有花生根瘤線蟲以及南方根瘤線蟲之複合感染現象。
Free-living, predatory and plant-parasitic nematodes that associated with the commercial submerged aquatic plants were surveyed in the study. Seventy-six species from 20 dicotylendonous families and 5 monocotylendonous families were investigated. Six families of free-living nematodes were found to associate with aquatic dicots in the families of Chronogasteridae, Diplopeltidae, Leptolaimidae, Mesorhabditidae, Rhabditidae and Tripylidae. The predatory nematodes in the families of Actinolaimidae, Anatonchidae, Cyanotholaimidae and Dorylaimidae were attained from aquatic dicots. Only one genus of plant-parasitic nematodes, identified as Aphelenchoides, was isolated from aquatic dicots, and the greatest amount of Aphelenchoides spp. were found from Cabomba aquatic plants. From monocotylendonous aquatic plants, free living nematodes in the families of Cephalobidae, Chronogasteridae, Leptopaimidae, Mesorhabditidae, Panagrolaimidae, Plectidae, Rhabditidae, and Tripylidae were detected. Nine families of predatory nematodes in the families of Actinolaimidae, Aporcelaimidae, Belondiridae, Dorylaimidae, Metateratocephalidae, Mononchidae, Mylonchulidae, Sphaeroliamidae and Tylencholaimellidae were found from aquatic monocots. Parasitic nematodes belonging to Aphelenchoides, Meloidogyne and Hirschmanniella were also found from aquatic monocots. Chronogateridae of free-living nematodes and Dorylaimidae of predatory nematodes were the two dominant families found in this survey, aquatic plants might provide the main food resources and a suitable habitat for Chronogateridae and Dorylaimidae populations. There were only three genus of plant-parasitic nematodes found in this survey. The morphology of Aphelenchoides spp. from C. aquatic was similar with an Aphelenchoides new species reported by Yang. There were only two Hirschmanniella individual females isolated in this survey which were not sufficient for species identification. Significant galling symptoms caused by Meloidogyne spp. were observed on the anubias roots. The perineal pattern had closed lines with lower and disorderly dorsal arch. Lateral ridges and subcuticular punctuations surrounding the anus were absent. The morphology of perineal pattern indicated the root knot nematodes might be M. arenaria. The mitochondria DNA sequence of COII region from five single female populations all yielded an approximately 1.7 kb fragment, and the SCAR-PCR yielded specific bands, indicated that the root knot nematodes were M. arenaria or M. incognita, respectively.
URI: http://hdl.handle.net/11455/31965
其他識別: U0005-0708201221020300
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