Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31988
標題: 臺灣水稻紋枯病菌Rhizoctonia solani AG-1 IA特性分析比較
Characterization and comparison of rice sheath blight pathogen Rhizoctonia solani AG-1 IA in Taiwan
作者: 方則淳
Fang, Che-Chun
關鍵字: 水稻紋枯病
rice sheath blight
Rhizoctonia solani AG-1 IA
病害檢定
表面覆膜技術
感染褥
Rhizoctonia solani AG-1 IA
disease assessment
surface membrane technique
infection cushion
出版社: 植物病理學系所
引用: 古德業、朱家彬。1977。臺灣常用殺菌劑對水稻紋枯病之藥效研究。臺灣農業季刊13(4):19~27。 行政院農業委員會農糧署。2011。臺灣地區農產品生產量值。http://www.afa.gov.tw/Public/GrainStatistics/20124301743155334.pdf 李新傳。1971。水稻紋枯病病株對水稻病原力之研究。高雄區農業改良場。 杜金池、張義璋。1978。Rhizoctonia solani Kuhn 之菌絲融合羣及其對水稻之病原性。邱人璋編。水稻病蟲害:生態學與流行學。263~285頁。 杜金池、張義璋、王仲文。1979。水稻紋枯病原菌之生態研究。科學發展月刊7(12):1208~1219。 杜金池、張義璋。1981。水稻紋枯病之生態及生物防治研究。台南區農改場研究彚報15:1~24。 杜金池、張義璋。1991。水稻紋枯病之生態及防治。農業試驗所特刊32:65~81。 吳龍溪。1971。稻紋枯病。p.46~76。邱人璋編。稻作病害。pp.371。農復會。 周而勛、曹菊香、楊媚、朱西儒。2002。我國南方六省(區)水稻紋枯病遺傳多樣性的研究。南京農業大學學報25(3):36~40。 陳治官。2002。前言-作物簡介。植物保護圖鑑系列-水稻保護(上冊)。p.2~8。行政院農業委員會動植物防疫檢疫局。 陳思豪。2007。台灣水稻紋枯病菌Rhizoctonia solani AG1-IA菌株之多樣性。中興大學植物病理研究所第三十七屆碩士論文。 張德前、劉達休。1984。低容量莖間施藥法防治水稻紋枯病與褐飛蝨之效果。臺中區農業改良場研究彙報9:22~28。 張義璋。1985。影響稻紋枯病菌生長及菌核形成之因子。中華農業研究34:454~463。 張義璋。2002。紋枯病。植物保護圖鑑系列-水稻保護(下冊)。p.299~310。行政院農業委員會動植物防疫檢疫局。 游俊明。2002。水稻疑似紋枯病。植物保護圖鑑系列-水稻保護(下冊)。p.279~289。行政院農業委員會動植物防疫檢疫局。 楊相哲。2006。台灣地區水稻紋病菌株差異性之研究。中興大學植物病理研究所第三十四屆碩士論文。 費雯綺、王喻其。2007。植物保護手冊。行政院農業委員會藥物毒物試驗所。台中。884頁。 臺灣稻作資訊系統。2011。育成品種。http://tris.tari.gov.tw:8080/default.jsp 鄭清煥。2002。前言-台灣水稻病、蟲、草害概述及防治管理。植物保護圖鑑系列-水稻保護(上冊)。p.8~12。行政院農業委員會動植物防疫檢疫局。 蔡武雄。1973。水稻紋枯病菌病原性之研究。臺灣省農業試驗所62年年報。p.134。 蔡武雄。1975a。水稻紋枯病不同發病程度與產量及產量損失關係之研究。植保會刊17(4):410-417。 蔡武雄。1975b。水稻品種品系對紋枯病抵抗性不同檢定方法之比較。農試所研究彙報32:22-28。 蔡武雄。1976。紋枯病對水稻產量損失估計。植保會刊18(2):106-119。 簡錦忠、鍾順昌、朱啟魯。1963。稻紋枯病菌菌核脫落之數量及其發芽試驗。中華農業研究12(2):7~13。 蔡武雄、游俊明。1978。水稻紋枯病之流行學及其對產量之影響。水稻病蟲害:生態學與流行學。247-262頁。邱人璋主編。 鄧耀宗、許東暉。1979。水稻抗紋枯病之研究。中華農業研究28(3):145~150。 簡錦忠、洪雲卿、劉鐵寧。1969。稻紋枯病病原菌菌核之病原性。中華農業研究18(4):19~23。 簡錦忠、洪雲卿。1971。水稻紋枯病之系統性藥劑防治試驗。中華農業研究20(2):37~46。 簡錦忠、洪雲卿、朱啟魯。1972。水稻紋枯病之系統性藥劑室內及田間防治試驗。中華農業研究21(3):191~202。 簡錦忠、朱啟魯。1973。系統性Benomyl劑對稻熱病稻紋枯病防治作用之研究。中華農業研究22(1):41~46。 深野弘・1930。稲紋枯病の細胞学的研究。九大農学部学芸雑誌5:117-136。 深津景栄、柿崎正、平山成一。1960。稲紋枯病の担胞子による二次伝染に関する研究。高知農試研究報告 2:26~38。 堀真雄。1967。稲紋枯病の被害予察と防除法。農業および園芸42:1389~1392。 堀眞雄。1991a。発生生態。イネ紋枯病-発生・防除の理論と実際-。堀眞雄著。東京,日本植物防疫協会。p.38~78。 堀眞雄。1991b。紋枯病類似病害。イネ紋枯病-発生・防除の理論と実際-。堀眞雄著。東京,日本植物防疫協会。p.235~250。 伊藤一雄、紺谷修治、近藤秀明。1955。カラマツ苗のくもの巣病菌。林業試験場研究報告 79:43~63。 中沢雅典、加藤喜重郎。1959。稲紋枯病菌の担胞子伝染について。関西病虫害研究会報 2:35~36。 宇井格生、斎藤全。1968。 Rhizoctonia 菌糸の細胞核数について。北海道大学農学部邦文紀要6(3):359~363。 渡辺文吉郎、松田明。1966。畑作物に寄生する Rhizoctonia solani Kuhn の類別に関する研究。農林水産技術会議指定試験(病害虫)7: 1–131. Abdel-salam, M. M. 1933. Damping-off and other allied diseases of lettuce. J. Pomol. Hort. Sci. 11: 259-275. Ahn, S. W., dela Pena, R. C., Candole, B. L., and Mew, T. W. 1986. A new scale for rice sheath blight (ShB) disease assessment. International Rice Research Newsletter 11(6): 17. Anderson, N. A. 1982. The genetics and pathology of Rhizoctonia solani. Annual Review of Phytopathology 20: 329- 47. Armentrout, V. N., and Downer, A. J. 1987. Infection cushion development by Rhizoctonia solani on cotton. Phytopathology 77: 619-623. Armentrout, V. N., Downer, A. J., Grasmik, D. L., and Weinhold, A. R. 1987. Factors affecting infection cushion development by Rhizoctonia solani on cotton. Phytopathology 77: 623-630. Akino, S., Ogoshi, A. 1995. Pathogenicity and host specificity in Rhizoctonia solani. In U.S. SINGH (Ed.), Pathogenesis and Host Specificity in Plant Diseases, Histopathological Biochemical, Genetic and Molecular Bases. Vol. II. Eukaryotes. Elsevier Science Inc., New York. p 7–49. Bandoni, R. J. 1979. Safranin O as a rapid nuclear stain for fungi. Mycologia 11(4): 873-874. Banniza, S., Sy, A. A., Bridge, P. D., Simons, S. A., and Holderness, M. 1999. Characterization of populations of Rhizoctonia solani in paddy rice fields in Cȏte d’Ivoire. Phytopathology 89: 414-420. Bassi, A. Jr., Moore, E. L., and Batson, W. E. Jr. 1979. Histopathology of resistant and susceptible tomato fruit infected with Rhizoctonia solani. Phytopathology 69: 556-559. Carling, D. E., Kuninaga, S., and Leiner R. H. 1988. Relatedness within and among intraspecific groups of Rhizoctonia solani: A comparison of grouping by anastomosis and by DNA hybridization. Phytoparasitica 16: 209-210. Channamallikarjuna, V., Sonah, H., Prasad, M., Rao, G. J. N., Chand, S., Upreti, H. C.,Singh, N. K., and Sharma, T. R. 2010. Identification of major quantitative trait loci qSBR11-1 for sheath blight resistance in rice. Mol. Breeding 25:155-166. Chiang, M. Y. 1995. Paddy weeds in Taiwan: an overall view on flora, ecology and control. Plant Prot. Bull. 37: 339-355. de Silva, R. L., and Wood, R. K. S. 1964. Infection of plans by Corticium solani and C. parasitica- effect of plant exudates. Trans. Brit. Mycol. Soc. 47: 15-24. Dodman, R. L., Barker, K. R., and Walker, J. C. 1968. A detailed study of the different modes of penetration by Rhizoctonia solani. Phytopathology 58: 1271-1276. Flentje, N. T. 1957. Studies on Pellicularia filamentosa (Pat.) Rogers III. Host penetration and resistance and strain specialization. Trans. Brit. Mycol. Soc.40: 322-336. Flentje, N. T., Dodman, R. L., and Kerr, A. 1963. The mechanism of host penetration by Thanatephorus cucumeris. Australian Journal of Biological Sciences 16: 784-799. Food and Agriculture Organization. 2010. FAOSTAT. (online) http://faostat.fao.org/site/291/default.aspx Fukutomi, M, and Takada, H. 1979. Ultrastructure of Infection Process of Rhizoctonia solarli Kuhn in Cucumber Hypocotyls. Ann. Phytopath. Soc. Japan 45: 453-462. Guleria, S., Aggarwal, R., Thind, T. S., and Sharma, T. R. 2007. Morphological and pathological variability in rice isolates of Rhizoctonia solani and molecular analysis of their genetic variability. Journal of Phytopathology 155: 654-661. Hashiba, T. 1982. Sclerotial morphogenesis in the rice sheath blight fungus (Rhizoctonia solani). Bull. Hokuriku Natl. Agr. Exp. Stn. 24: 29-83 Hirooka, T., Mayagi, Y., Araki, F., and Kunoh, H. 1989. Biological mode of action of flutolanil in its systemic control of rice sheath blight. Phytopathology 79: 1091-1094. Hofman, T. W., and Jongebloed, P. H. J. 1988. Infection process of Rhizoctonia solani on Solanum tuberosum and effects of granular nematocides. Neth. J. Plant Pathol. 94: 243-252. Hyakumachi, M., and Ui, T. 1987. Non-self-anastomosing isolates of Rhizoctonia solani obtained from fields of sugarbeet monoculture. Trans. Br. Mycol. Soc. 89: 27-33. International Rice Research Institute. 2009. Rice doctor (online) - Informationa sheets – Sheath blight. http://www.knowledgebank.irri.org/RiceDoctor/ Jia, Y., Liu, G., Costanzo, S., Lee, S., and Dai, Y. 2009. Current progress on genetic interactions of rice with rice blast and sheath blight fungi. Front. Agric. China 3(3): 231-239. Keijer, J. 1996. The initial steps of the infection process in Rhizoctonia solani. In: Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. Edited by :Sneh, B., Jabaji-Hare, S., Neate, S. and Dijst, G, pp. 149–162. Dordrecht: Kluwer Academic Publishers. Kerr, A. 1955. Some interactions between plant roots and pathogenic soil fungi. Australian Journal of Biological Sciences 9: 45-52. Khodayari, M., Safaie, N., and Shamsbakhsh, M. 2009. Genetic diversity of Iranian AG1-IA isolates of Rhizoctonia solani, the cause of rice sheath blight, using morphological and molecular markers. Journal of Phytopathology 157: 708-714. Kim, H. T., Chung, Y. R., and Cho, K. Y. 2001. Mycelial melanization of Rhizoctonia solani AG1 affecting pathogenicity in rice. Plant Pathol. J. 17(4): 210-215. Kousik, C. S., Snow, J. P., Berggren, G. T. 1994. Factors affecting infection cushion development by Rhizoctonia solani AG-1 IA and IB on soybean leaves. Plant Pathology 43: 237-244. Kronland, W. C., and Stanghellini, M. E. 1988. Clean slide technique for the observation of anastomosis and nuclear condition of Rhizoctonia solani. Phytopathology 78: 820-822. Liu, G., Jia, Y., Correa-Victoria, F. J., Prado, G. A., Yeather, K. M., McClung, A., and Correll, J. C. 2009. Mapping quantitative trait loci responsible for resistance to sheath blight in rice. Phytopathology 99: 1078-1084. Mahmoud, Y. A. G., Gaafar, R. M., and Mubarak, H. M. 2007. Genetic diversity among Nile Delta isolates of Rhizoctonia solani Kuhn based on pathogenicity, compatibility, isozyme analysis and total protein pattern. Turk. J. Bot. 31: 19-29. Marshall, D. S., and Rush, M. C. 1980. Relation between infection by Rhizoctonia solani and R. oryzae and disease severity in rice. Phytopathology 70: 941-946. Matsuura, K. 1986. Scanning electron microscopy of the infection process of Rhizoctonia solani in leaf sheaths of rice plants. Phytopathology 76: 811-814. Mew, T. W., Leung, H., Savary, S., Vera Cruz, C. M., and Leach, J. E. 2012. Looking ahead in rice disease research and management. Plant Sciences 103-127. Nakayama, T. 1940. A study on the infection of cotton seedlings by Rhizoctonia solani. Ann. Phytopathol. Soc. Japan 10: 93-103. Parmeter, J. R. Jr., Sherwood, R. T., and Platt, W. D. 1969. Anastomoss grouping among isolates of Thanatephorus cucumeris. Phytopathology 59:1270-1278. Priyatmojo, A., Escopalao, V. E., Tangonan, N. G., Pascual, C. B., Suga, H., Kageyama, K., and Hyakumachi, M. 2001. Characterization of a new subgroup of Rhizoctonia solani anastomosis group 1 (AG-1-ID), causal agent of a necrotic leaf spot on coffee. Phytopathology 91:1054-1061. Richter, H., und Schneider, R. 1953. Untersuchungen zur morphologischen und biologischen Differenzierung von Rhizoctonia solani K. Phytopath. Z. 20:165-226. Rosewich, U. L., Pettway, R. E., McDonald, B. A., and Kistler, H. C. 1999. High levels of gene flow and heterozygote excess characterize Rhizoctonia solani AG-1 IA (Thanatephorus cucumeris) from Texas. Fungal Genetics and Biology 28: 148-159. Savary, S., Willocquet, L., Elazegui, F. A., Castilla, N. P., and Teng, P. S. 2000. Rice pest constraints in tropical Asia: Quantification of yield losses due to rice pests in a range of production situations. Plant Disease 84:357-369. Schultz, H. 1936. Vergleichende Untersuchungen zur Okologie, Morphologie und Systematik des" Vermehrungspilzes". Arb. Biol. Reichsanst. Fur Landund Forstwirtsch. (Berlin) 22:1-41. Sharma, A., McClung, A. M., Pinson, S. R. M., Kepiro, J. L., Robert-Shank, A., Tabien, R. E., and Fjellstrom, R. 2009. Genetic mapping of sheath blight resistance QTLs within tropical Japonica rice cultivars. Crop Science 49: 256-264. Sharon, M., Kuninaga, S., Hyakumachi, M., Naito, S., and Sneh, B. 2008. Classifi cation of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping. Mycoscience 49: 93-114. Sneh, B., Burpee L., and Ogoshi, A. 1991. Identification of Rhizoctonia Species. A.P.S. Press. 133 pp. Srinivasachary, Willocquet, L., and Savary, S. 2010. Resistance to rice sheath blight (Rhizoctonia solani Kuhn) [teleomorph: Thanatephorus cucumeris (A. B. Frank) Donk.] disease: current status and perspectives. Euphytica 178(1): 1-22. Sumner, D. R. 1996. Sclerotia formation by Rhizoctonia species and their survival. In Rhizoctonia Species : Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. Edited by: Sneh, B., Jabaji-Hare, S., Neate, S. and Dijst, G. pp. 207-216, Kluwer Academic Publisher, The Netherlands. Taheri, P., Gnanamanickam, S., and Hofte, M. 2007. Characterization, genetic structures, and pathogenicity of Rhizoctonia spp. Associated with rice sheath blight in India. Phytopathology 97: 373-383. United States Department of Agriculture, Natural Resources Conservation Service. 2012. The PLANTS Database (online). National Plant Data Team, Greensboro, North Carolina. http://www.plants.usda.gov/java/ Wu, W. S. 1978. Parasitism of the pathogen of the rice sheath blight. Plant Prot. Bull. 20: 151-160. Xiao, Y., Liu, M. W., Li, G., Zhou, E. X., Wang, L. X., Tang, J., Tan, F. R., Zheng, A. P., and Li, P. 2008. Genetic diversity and pathogenicity variation in Rhizoctonia solani isolates from rice in Sinchuan province, China. Rice Science 15(2): 137-144. Yamamoto, D. T., and Uchida, J. Y. 1982. Rapid nuclear staining of Rhizoctonia solani and related fungi with acriding orange and with safranin. Mycologia 74: 145-149. Yang, J., Verma, P. R., and Lees, G. L. 1992. The role of cuticle and epidermal cell wall in resistance of rapeseed and mustard to Rhizoctonia solani. Plant and soil 142: 315-321. Yu, C. M., Ling, K. C., and Ou, S. H. 1976. Effect of nutritional and microclimatic conditions on the development of sheath blight disease of rice. Plant Prot. Bull. 18(3): 261-267.
摘要: 水稻紋枯病是由絲核菌第一融合群亞群(Rhizoctonia solani AG-1 IA)所引起,主要發生在高溫高濕氣候的地區,於臺灣已為常見之本土性水稻病害。藉由了解水稻紋枯病菌其生長特性、菌核分佈及菌核大小等差異性,並進一步觀察對水稻品種之致病能力,再觀察病原菌感染構造,進行上述特性之比較分析。從臺灣各地收集之水稻紋枯病菌株,其生長速率介於0.37~1.95mm / hr,整體平均生長速率為1.5 mm / hr。各菌株之菌核直徑則介於0.089±0.021~0.244±0.074cm之間。菌落型態可依照菌落褐化程度、菌核排列形式及菌核數量大小等特性進行區分。菌核分佈形式可分為六類,而菌落依照褐化程度可分為微弱、普通及劇烈三種類型。菌株群中呈現最高之菌核量為每平方公分含1.768±0.038顆,最少則為0.078±0.059顆。將上述菌株生長特性與菌株來源區域之年均溫、年降雨進行配對後,發現菌絲生長速率與年均溫呈正相關,而菌核數量則與年雨量呈正相關,年雨量則與菌核大小關聯性不高,反之則與年均溫較有關聯。將水稻紋枯病菌接種於插秧後50天之臺南11號並於接種一個月後統計發病指數,各紋枯病菌株造成之病徵其平均高度介於41.8±0.44~59.5±0.52 cm之間,造成之相對病斑高度範圍為44.8~66%,造成之平均病害指數範圍為3~9級。各菌株之菌落色素深淺及生長速率與其造成之植株病斑高度呈正相關。利用半透膜覆蓋水瓊脂及稻稈煎汁瓊脂培養基,觀察接種水稻紋枯病菌株後的菌絲發展及感染構造之產生狀況。水瓊脂培養基處理中盤據效率差,鮮少發現任何分化的構造或典型的感染褥產生,而有少數葉狀附著器存在。水稻稻稈煎汁瓊脂培養基處理中可發現菌絲纏聚較茂密,少部分區域有不明顯的菌絲聚集,類似典型的感染構造。由於誘導效果不佳,因此直接覆蓋臺南11號水稻稻桿,接種後兩天可以發現隔著半透膜之稻桿位置上方的菌絲呈現高度聚集,和未接觸稻桿組織之半透膜區域菌絲形成明顯對比,又之後發現菌絲接觸稻稈的位置後皆會於上方形成菌絲聚集。而菌絲可以直接穿透半透膜的脆弱區域,並碰觸到寄主表面之後產生特化構造。於接種臺南11號稻叢進行發病觀察同時對病組織進行取樣以觀察感染構造。菌株間之感染構造皆為典型之感染褥,由菌絲分支聚集、交叉堆疊而成,另外也會發現聚集較不複雜,單一菌絲膨大且分支的小型感染構造,類似葉狀附著器。整體感染構造之呈現面積介於357.53~1696.97μm2,而每平方公厘之感染構造數目為35.62~118.98個。將上述個別項目之觀察數據與各菌株之平均發病指數進行關聯性分析,感染構造面積及單位面積感染構造數目與發病指數之間呈正相關。若藉由上述之相關性,進一步探討水稻紋枯病菌株接種不同齡期水稻稻株或不同接種方式所產生之發病情形,若能與一般抗性檢定之數據相關,則可幫助減少耗時及其他成本以幫助檢定。而各地區之水稻紋枯病菌其部分特性對於各地區環境相關,結合水稻紋枯病菌之生態性,進行水稻栽培方式之調整,期能對病害整合管理上提供參考。
Rice sheath blight disease caused by Rhizoctonia solani AG-1 IA always occur in the area with high temperature and moisture and has already become endemic in Taiwan. This study analyzed the difference and relationship of the morphology, mycelial growth, sclerotial arrangement, the virulence and infection structure. Rice sheath blight isolates collected in Taiwan showed the growth rate 0.37~1.95 mm / hr, and the average growth rate was 1.5mm/hr; the isolates showed the sclerotial diameter 0.089±0.021-0.244±0.074cm. The colony could be classified with three criteria including sclerotial number, sclerotial arrangement with 6 types, and the colony pigmentation that could be divided into weak, normal, and obvious levels. Sclerotial number produced by isolates ranges between 1.768±0.038-0.078±0.059 number / cm2. After comparing the characteristics of isolates with the environmental condition of collection source, mycelia growth rate shows the positive correlation with annual mean temperature, and sclerotial number shows the positive correlation with annual rainfall. However, the sclerotial diameter shows no correlation with annual rainfall, but with annual mean temperature. Inoculation to cultivar Tainan 11 with isolates was carried out for observation of the virulence and all isolates could cause the disease severity 3-9, with the average lesion height 41.8±0.44-59.5±0.52 cm and the relative lesion height 44.8~66%. The mycelia growth rate and the colony pigmentation show the positive correlation with the lesion height. Surface membrane technique was used to cover the water and rice sheath water extract agar medium for the comparison of infection structure induction, and the latter showed the better effect on colonization promotion, but the infection structure was not obvious and typical. By using the sterilized rice sheath tissue instead of the agar medium, the hyphal aggregation occurred on the location attached with rice sheath tissue, and may penetrated through the fragile part, then attach to the rice tissue surface. The mycelia differentiated following the attachment with tissue surface. The diseased tissue was sampled from the virulence test to Tainan 11 for direct observation of infection structure. All isolates showed the typical infection cushion and some swelling, curving hypha were investigated like the structure lobate appressoria. The area of infection structure ranged between 357.53-1696.97μm2 and the number of infection structure was 35.62~118.98 number / mm2. The area and the number of infection structure show the positive correlation with the disease severity. According to the relatedness analysis, different disease assessment for the resistance could be tested and confirmed the similarity and the trend with the standard rice cultivar evaluation of rice sheath blight resistance currently. And other relatedness of characteristics with environmental factors could be combined with the ecology of rice sheath blight pathogen and the rice crop system, for modifying the strategy and achieve integrated pest management.
URI: http://hdl.handle.net/11455/31988
其他識別: U0005-2408201215044800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2408201215044800
Appears in Collections:植物病理學系

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