Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3219
標題: 應用聚丙烯腈基材之固定化金屬親和薄膜於蛋白質吸附
Preparation of Polyacrylonitrile-Based Immobilized Metal-Ion Affinity Membrane for Protein Adsorption
作者: 楊茵傑
Yang, Yin-Jie
關鍵字: 聚丙烯腈固定化金屬
Polyacrylonitrile membrane
蛋白質
IMAC
Protein
出版社: 化學工程學系所
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摘要: 本研究以聚丙烯腈薄膜為載體,利用乙二胺、乙二醇二縮水甘油醚改質薄膜上的表面官能基,進而固定螯合劑亞氨基二乙酸和銅離子,製備成固定化金屬親和薄膜。使用ATR-FTIR、SEM定性分析成功改質後的薄膜性質,再以ICP、UV-Vis分析銅離子和蛋白質Lysozyme、BSA的吸附量,以及螢光光譜儀檢測綠螢光蛋白(GFP)的吸附量。結果顯示:最佳乙二胺反應條件為60oC、3小時,最佳乙二醇二縮水甘油醚反應條件為60oC、4小時,此時銅離子最大鍵結量為4.8 umol/cm2,而Lysozyme、BSA、GFP吸附量分別為510、69、72 ug/cm2。本研究亦計算出每個蛋白質吸附所佔據的表面積,Lysozyme(分子大小3.0×3.0×4.5 nm)為0.95 nm2,判斷可能為多層吸附;BSA(分子大小4.0×4.0×14 nm)和GFP(分子大小為直徑3 nm、長度4 nm)分別為36及19 nm2,應為單層吸附,並可據以了解其吸附於薄膜上的構形。
In this study, a five-step approach was designed to prepare an immobilized metal-ion affinity membrane (IMAM) having a high capacity for protein adsorption. Polyacrylonitrile (PAN) membranes were selected as substrates due to their excellent thermal stability and solvent resistance. The -CN groups on PAN membranes were converted to -COOH groups by immersion into 3 N NaOH at 85 oC for 10 min, then 1 M HCl at 25 oC for 2 h. Next, the membranes were chemically modified by reacting with ethylenediamine (EDA) and ethylene glycol diglycidyl ether (EGDGE) to produce terminal epoxy groups. Iminodiacetic acid (IDA) was bound to the above modified PAN membranes (0.2 M or 1 M IDA and 1 M Na2CO3, pH 11) at 80 oC for 12 h and further chelated with copper ions (0.05 M or 0.5 M CuSO4) at 25 oC for 4 h. The optimal conditions were found to be 60 oC and 3 h for EDA reaction, as well as 60 oC and 4 h for EGDGE reaction. The resultant copper ion capacity was 250 umol/ml (1.5 umol/mg) under these optimized conditions, and the related lysozyme, BSA and GFP adsorption capacity were 510 ug/cm2, 69 ug/cm2 and 72 ug/cm2, respectively. By further increasing the IDA and CuSO4 concentrations to 1 M and 0.5 M, the lysozyme and BSA adsorption capacity were improved to 517 (ug/cm2) and 82 (ug/cm2), respectively. By calculation, the membrane surface area occupied by one BSA molecule is 36 nm2, close to its molecular size. However, the value for lysozyme is 0.95 nm2, indicating a jam-packed adsorption.
URI: http://hdl.handle.net/11455/3219
其他識別: U0005-3007201212561000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-3007201212561000
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