Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3225
標題: 杏鮑菇液態醱酵培養條件生產胞外多醣體及其特性探討
Study of culture conditions on exopolysaccharide production and its characteristic by Pleurotus eryngii in the submerged culture
作者: 陳華彬
Chen, Hua-Ping
關鍵字: 杏鮑菇菌
Pleurotus eryngii
液態培養
多醣體
杏鮑菇水解粉
發光二極體
廢棄物處理
submerged culture
exopolysaccharide
mushroom hydrolysate powder
LED
waste treatment
出版社: 化學工程學系所
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摘要: 菇類含有許多生物活性物質,如多醣體、三萜類、麥角固醇、核苷酸、蛋白多醣、核酸、食用纖維等,具有抗腫瘤、免疫調節、降血壓、降血糖、降膽固醇、抗細菌及病毒、延緩骨質疏鬆等保健功效,非常有發展潛力。以液態培養方式則有培養週期較短、產品品質較穩定、易於商業化大規模生產等優點。因此,利用液態培養方式進行杏鮑菇菌生物活性物質的開發與應用是極值得進行的研究題材。 本論文主要包含三個部分。第一部分,為培養基質及製備杏鮑菇水解粉(MHP)對杏鮑菇菌絲體生長與多醣體生產之探討。其結果顯示,以葡萄糖(2%)及MHP(1.6%) 為最佳碳、氮源供以菌絲體及胞外多醣體生產,其比生長速率(specific growth rate)及比胞外多醣體產率(specific EPS yield) 分別為0.68 day-1及 9.55 g/g-dry-cell-weight ,而其菌絲體生長速率及胞外多醣體產量分別為2.05 g/L/day及 92 mg/L。於5公升醱酵槽放大試驗中,其菌絲體生長速率及胞外多醣體產量為2.44 g/L/day 及312 mg/L。 於固態培養時,使用MHP 培養基(6.43±0.21 mm/day)其菌絲生長速度比馬鈴薯培養基(4.57±0.23 mm/day) 快速,藉由不同濃度MHP調整,其高低分子量之胞外多醣體亦可隨之調整其比例,由PCA及cluster 分析結果,其MHP主要胺基酸與杏鮑菇菌絲體水解粉(PEMHP)相當接近。 第二部分於杏鮑菇液態醱酵培養過程中,針對不同光波長之LED燈進行照射,於固態培養時,紅光最有助於菌絲體生長,其菌絲生長速率為5.36 mm/day,於液態醱酵培養時,使用藍光可得到最大胞外多醣體之產量,其值為455 mg/L,為不照光提升7.71倍,使用紅光及黃光時,有助於得到高分子量(1000~1200 kDa)之胞外多醣體。 第三部分於杏鮑菇液態醱酵培養過程中,分別使用不同添加劑(如:有機酸、醇類、植物油與界面活性劑等)加入培養基中,探討添加劑與杏鮑菇液態醱酵培養菌絲體生長與多醣體生產之影響,以期促進杏鮑菇菌絲體生長與多醣體生產之效率。結果顯示,添加異丙醇為最佳提升胞外多醣體產量之添加物,異丙醇濃度在0.5 %下為最佳值,其胞外多醣體可達215 mg/L,為未加添加前提升3.47倍。不同時間添加異丙醇於杏鮑菇培養時之菌絲體產量並無顯著性差異,於第2天時添加可獲得最高之胞外多醣體產量,其值可達240 mg/L,為第0天添加提升約10.4% 之胞外多醣體產量。
Mushrooms contains many biologically active compounds such as polysaccharides, triterpenes, ergosterol, nucleotide, protein polysaccharides, nucleic acids and dietary fiber, which provide anti-tumor, immune regulation, lowering blood pressure and sugar, lowering cholesterol, anti-bacteria and anti-viruses, osteoporosis postponing and other health effects. The submerged culture of mushrooms is expected to reduce cultivation time, stabilize product quality, and realize the scale-up process. This study contains three parts. In the first part, Pleurotus eryngii head part waste in a bag-log was collected and processed to yield mushroom hydrolysate powder (MHP). To test its functional use, a P. eryngii submerged culture was conducted. The prepared MHP (1.6%) and glucose (2%) were found to be the best sources for both mycelial biomass and exopolysaccharide (EPS) productions. A specific growth rate of 0.68 day-1, and a specific EPS yield of 9.55 g/g-dry-cell-weight were obtained under these conditions. It was also found that MHP gave higher mycelial biomass growth rate (2.05 g/L/day), and EPS yield (92 mg/L) than those of yeast extract. In 5-L scale-up fermentation with MHP as nitrogen source, high mycelial biomass growth rate (2.44 g/L/day) and EPS yield (312 mg/L) were obtained. In the solid state culture, P. eryngii hyphae growth rate in MHP agar (6.43�0.21 mm/day) was better than that in PDA (4.57�0.23 mm/day). By using PCA and cluster analysis, the major amino acids of MHP displayed a close similarity to those of P. eryngii mycelial hydrolysate powder. In the second part, different LED lights were used in the P. eryngii culture. In the solid state culture, the red LED was found to be the most helpful for hyphae growth with its growth rate reaching 5.36 mm/day. In the submerged culture, the maximum EPS yield (455 mg/L) can be obtained when using blue LED, a 7.7-fold improvement as compared to the dark condition. It was found the high molecular weight EPS (1000~1200 kDa) fraction was produced when using red and yellow LEDs. In the third part, several additives such as organic acid, alcohols, vegetable oils and surfactants were applied to the P. eryngii submerged culture. The stimulatory effects on mycelial biomass and EPS production were studied. It was observed that 2-propanol showed the best stimulatory effect for EPS production with its yield reaching 215 mg/L. A 3.47-fold EPS increase could be obtained when adding 0.5% of 2-propanol, whereas, no significant increase on P. eryngii mycelial biomass was found in the same addition. In contrast, the highest EPS yield (240 mg/L) was obtained when 5% of 2-propanol was added at day 2, accounting for a 10.4% EPS yield increase as compared to that without 2-propanol addition.
URI: http://hdl.handle.net/11455/3225
其他識別: U0005-2507201222392700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2507201222392700
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