Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32675
標題: Haemoglobin in normal and neoplastic canine mammary glands
作者: Chang, S.C.
王孟亮
Chen, H.F.
Chou, M.H.
Wang, H.C.
Su, H.Y.
Wong, M.L.
張仕杰
關鍵字: canine
haemoglobin
mammary gland
mammary tumor
Western blot
nitric-oxide
cytoglobin
neuroglobin
cells
hypoxia
期刊/報告no:: Veterinary and Comparative Oncology, Volume 8, Issue 4, Page(s) 302-309.
摘要: Four types of globins for oxygen transport are known in vertebrates, and the haemoglobin is responsible for carrying oxygen in blood. In this study, we found that haemoglobin was also expressed in canine mammary glands. Samples were taken from 26 malignant mammary tumors, 16 normal mammary glands and 10 other normal tissues. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and mass spectrometry were used to investigate haemoglobin in mammary tissues. The results indicated that normal canine mammary glands expressed high levels of haemoglobin protein as shown by Coomassie blue staining. The identity of haemoglobin was confirmed by immunoblotting and mass spectrometry, and the mass spectrometry data revealed that both alpha-haemoglobin and beta-haemoglobin were expressed. Relative to normal mammary glands, the levels of haemoglobin expression in mammary tumors were lower. Our results also indicated that the haemoglobin was endogenously produced in mammary gland tissues and was not derived from the erythroid cells.
URI: http://hdl.handle.net/11455/32675
ISSN: 1476-5810
文章連結: http://dx.doi.org/10.1111/j.1476-5829.2010.00229.x
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