Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32686
標題: DuraSeal as a Ligature in the Anastomosis of Rat Sciatic Nerve Gap Injury
作者: Lin, K.L.
何素鵬
Yang, D.Y.
Chu, I.M.
Cheng, F.C.
Chen, C.J.
Ho, S.P.
Pan, H.C.
關鍵字: sciatic nerve injury
peripheral nerve regeneration
tissue glue
suture
DuraSeal
CatWalk
brachial-plexus injury
catwalk gait analysis
functional recovery
fibrin glue
in-vitro
regeneration
repair
adhesives
lesions
alpha
期刊/報告no:: Journal of Surgical Research, Volume 161, Issue 1, Page(s) 101-110.
摘要: Background. Anastomosis of the nerve especially at narrow surgical field and presence of surgical tension is not easily accessible. DuraSeal demonstrates strong adhesive power without producing neurotoxicity. Herein, we evaluate the possibility of DuraSeal as a substitute in the repair of sciatic nerve gap injury. Materials and Methods. The nerve gap model was constructed by excising the sciatic nerve (5 mm in length) in Sprague Dawley rats leaving a 5 mm nerve defect between nerve stumps. Animals were categorized into four groups: Group I: no treatment; Group II: 4 stitches suture; Group III: nerve approximation fixed by tissue glue; Group IV: nerve approximation fixed by DuraSeal. The motor function assessment included the CatWalk and SFI as well as electrophysiological studies. Nerve continuity and regeneration was examined at 1 and 8 wk after injury. The inflammatory cells, Schwann cell apoptosis, and Schwann cell proliferation were also investigated 1 wk after injury. Results. The achievement of nerve continuity and myelination by DuraSeal approached that of suture demonstrated by crystal violet and Luxol Fast Blue staining at 1 and 8 wk, respectively. Motor function and electrophysiological parameters were restored in DuraSeal and suture group. Early expression of neurofilament and bromodeoxyuridine (BrdU) was also observed in these two groups. There was no statistically significant difference in deposits of macrophages and neutrophil cells or cell apoptosis among these four groups. Conclusions. DuraSeal achieved the same nerve regeneration compared with that of suture and produced better regeneration than that of the tissue glue or without any treatment. The accomplishment of nerve regeneration and continuity without causing neurotoxicity justifies using DuraSeal as a ligature in the anastomosis of nerve gap injury. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/32686
ISSN: 0022-4804
文章連結: http://dx.doi.org/10.1016/j.jss.2008.10.020
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