Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32718
標題: Role of oxidative stress in thuringiensin-induced pulmonary toxicity
作者: Tsai, S.F.
何素鵬
Yang, C.
Liu, B.L.
Hwang, J.S.
Ho, S.P.
關鍵字: thuringiensin
oxidative stress
glutathione
pulmonary toxicity
protein-kinase-c
epithelial permeability
glutathione levels
alpha-tocopherol
free-radicals
tnf-alpha
kappa-b
lung
disease
expression
期刊/報告no:: Toxicology and Applied Pharmacology, Volume 216, Issue 2, Page(s) 347-353.
摘要: To understand the effect of thuringiensin on the lungs tissues, male Sprague-Dawley rats were administrated with thuringiensin by intratracheal instillation at doses 0.8, 1.6 and 3.2 mg/kg of body weight, respectively. The rats were sacrificed 4 h after treatment, and lungs were isolated and examined. Subsequently, an effective dose of 1.6 mg/kg was selected for the time course study (4, 8, 12, and 24 h). Intratracheal instillation of thuringiensin resulted in lung damage, as evidenced by increase in lung weight and decrease in alkaline phosphatase (10-54%), an enzyme localized primarily in pulmonary alveolar type 11 epithelial cells. Furthermore, the administration of thuringiensin caused increases in lipid peroxidation (21-105%), the indices of lung injury. In addition, the superoxide dismutase (SOD) and glutathione (GSH) activities of lung tissue extracts were measured to evaluate the effect of thuringiensin on antioxidant defense system. The SOD activity and GSH content in lung showed significant decreases in a dose-related manner with 11-21% and 15-37%, respectively. Those were further supported by the release of proinflammatory cytokines, as indicated by increases in IL-1 beta (229-1017%) and TNF-alpha (234%) levels. Therefore, the results demonstrated that changes in the pulmonary oxidative-antioxidative status might play an important role in the thuringiensin-induced lung injury. (c) 2006 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/32718
ISSN: 0041-008X
文章連結: http://dx.doi.org/10.1016/j.taap.2006.05.013
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