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標題: DNA vaccination induces a long-term antibody response and protective immunity against pseudorabies virus in mice
作者: Ho, T.Y.
Hsiang, C.Y.
Hsiang, C.H.
Chang, T.J.
關鍵字: direct gene-transfer
vaccinia virus
mouse muscle
plasmid dna
期刊/報告no:: Archives of Virology, Volume 143, Issue 1, Page(s) 115-125.
摘要: In order to investigate the mechanism of long-term immunity and the effect of protective immunity induced by DNA vaccination, we constructed the expression plasmid containing a pseudorabies virus (PRV) gD gene encoding an envelope glycoprotein. Intramuscular vaccination of mice with the plasmid DNA induced a strong antibody response which lasted for one year after final vaccination. An IgM to IgG class switch occurred, indicating helper T-lymphocyte activity. We further analyzed the persistence and expression of gD gene by polymerase chain reaction and reverse transcriptase polymerase chain reaction. The results showed that gD gene was present and expressed in the muscle cell up to one year after final booster injection. Furthermore, mice vaccinated with the plasmid DNA were protected against a subsequent lethal challenge with PRV. Therefore, the DNA vaccination does induce a protective immunity and long-term antibody response against PRV, which could be maintained by persistent expression of gD gene in muscle cells.
ISSN: 0304-8608
Appears in Collections:獸醫學系所



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