Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32807
標題: Programmed cell death induced by Japanese encephalitis virus YL vaccine strain or its recombinant envelope protein in varied cultured cells
作者: Chen, S.O.
張天傑
Chang, T.J.
Stone, G.
Chen, C.H.
Liu, J.J.
關鍵字: JEV-YL
recombinant envelope protein
apoptosis
flavivirus
mice
expression
virulence
cloning
system
genome
cdna
期刊/報告no:: Intervirology, Volume 49, Issue 6, Page(s) 346-351.
摘要: Objective: The Japanese encephalitis virus YL vaccine strain (JEV-YL) was investigated as regards its organ tropism and the role of recombinant envelope glycoprotein in the induction of apoptosis was explored. Methods: Three varied cell lines (HepG2, Vero and C6) were infected with JEV-YL or transfected with eukaryotic expression plasmids (pcE, pcF1R2, pcF1R1 and pcF2R2) which contain different parts of the envelope gene and phenotypic properties were examined by flow cytometry and DNA fragmentation analysis. Results: After JEV-YL infection, smaller plaque was produced on HepG2 cells than on Vero cells, whereas no cytopathic effect was observed on C6 cells; moreover, by apoptosis and DNA fragmentation assays, the hallmark cytopathic effects were detected in HepG2 and Vero cells but not in C6 cells. Furthermore, cells used in our study transfected with recombinant core plasmid, pcE, which include full-length E gene but the deleted forms (pcF1R2, pcF1R1 and pcF2R2) did not have similar results as JEV-YLs. Conclusions: The JEV-YL vaccine strain had changed cell tropism to liver cells different from other virulent strains which have neural tropism, and in this study we proved that the transient-expressed entire E protein of JEV-YL could induce apoptosis and the mutations of E protein may change the organ tropism of JEV-YL. Copyright (c) 2006 S. Karger AG, Basel.
URI: http://hdl.handle.net/11455/32807
ISSN: 0300-5526
文章連結: http://dx.doi.org/10.1159/000095154
Appears in Collections:獸醫學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.